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Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres
W. Chen, D. Šmajs, Y. Hu, W. Ke, P. Pospíšilová, KL. Hawley, MJ. Caimano, JD. Radolf, A. Sena, JD. Tucker, B. Yang, JJ. Juliano, H. Zheng, JB. Parr
Language English Country United States
Document type Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
Grant support
K24 AI134990
NIAID NIH HHS - United States
R01 AI026756
NIAID NIH HHS - United States
U19 AI144177
NIAID NIH HHS - United States
NLK
Free Medical Journals
from 1997 to 1 year ago
Open Access Digital Library
from 1997-04-01
- MeSH
- Rabbits MeSH
- Humans MeSH
- Whole Genome Sequencing MeSH
- Syphilis * diagnosis microbiology MeSH
- Treponema pallidum classification genetics MeSH
- Chancre * diagnosis microbiology MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, N.I.H., Extramural MeSH
- Geographicals
- China MeSH
BACKGROUND: Whole-genome sequencing (WGS) of Treponema pallidum subspecies pallidum (TPA) has been constrained by the lack of in vitro cultivation methods for isolating spirochetes from patient samples. METHODS: We built upon recently developed enrichment methods to sequence TPA directly from primary syphilis chancre swabs collected in Guangzhou, China. RESULTS: By combining parallel, pooled whole-genome amplification with hybrid selection, we generated high-quality genomes from 4 of 8 chancre-swab samples and 2 of 2 rabbit-passaged isolates, all subjected to challenging storage conditions. CONCLUSIONS: This approach enabled the first WGS of Chinese samples without rabbit passage and provided insights into TPA genetic diversity in China.
Department of Biology Faculty of Medicine Masaryk University Brno Czech Republic
Departments of Genetics and Genome Sciences and Immunology UConn Health Farmington Connecticut USA
Dermatology Hospital Southern Medical University Guangzhou China
References provided by Crossref.org
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