Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres

W. Chen, D. Šmajs, Y. Hu, W. Ke, P. Pospíšilová, KL. Hawley, MJ. Caimano, JD. Radolf, A. Sena, JD. Tucker, B. Yang, JJ. Juliano, H. Zheng, JB. Parr

. 2021 ; 223 (5) : 848-853. [pub] 20210303

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22012508

Grantová podpora
K24 AI134990 NIAID NIH HHS - United States
R01 AI026756 NIAID NIH HHS - United States
U19 AI144177 NIAID NIH HHS - United States

BACKGROUND: Whole-genome sequencing (WGS) of Treponema pallidum subspecies pallidum (TPA) has been constrained by the lack of in vitro cultivation methods for isolating spirochetes from patient samples. METHODS: We built upon recently developed enrichment methods to sequence TPA directly from primary syphilis chancre swabs collected in Guangzhou, China. RESULTS: By combining parallel, pooled whole-genome amplification with hybrid selection, we generated high-quality genomes from 4 of 8 chancre-swab samples and 2 of 2 rabbit-passaged isolates, all subjected to challenging storage conditions. CONCLUSIONS: This approach enabled the first WGS of Chinese samples without rabbit passage and provided insights into TPA genetic diversity in China.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22012508
003      
CZ-PrNML
005      
20220506130027.0
007      
ta
008      
220425s2021 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1093/infdis/jiaa449 $2 doi
035    __
$a (PubMed)32710788
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Chen, Wentao $u Dermatology Hospital, Southern Medical University, Guangzhou, China
245    10
$a Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres / $c W. Chen, D. Šmajs, Y. Hu, W. Ke, P. Pospíšilová, KL. Hawley, MJ. Caimano, JD. Radolf, A. Sena, JD. Tucker, B. Yang, JJ. Juliano, H. Zheng, JB. Parr
520    9_
$a BACKGROUND: Whole-genome sequencing (WGS) of Treponema pallidum subspecies pallidum (TPA) has been constrained by the lack of in vitro cultivation methods for isolating spirochetes from patient samples. METHODS: We built upon recently developed enrichment methods to sequence TPA directly from primary syphilis chancre swabs collected in Guangzhou, China. RESULTS: By combining parallel, pooled whole-genome amplification with hybrid selection, we generated high-quality genomes from 4 of 8 chancre-swab samples and 2 of 2 rabbit-passaged isolates, all subjected to challenging storage conditions. CONCLUSIONS: This approach enabled the first WGS of Chinese samples without rabbit passage and provided insights into TPA genetic diversity in China.
650    _2
$a zvířata $7 D000818
650    12
$a tvrdý vřed $x diagnóza $x mikrobiologie $7 D002601
650    _2
$a lidé $7 D006801
650    _2
$a králíci $7 D011817
650    12
$a syfilis $x diagnóza $x mikrobiologie $7 D013587
650    _2
$a Treponema pallidum $x klasifikace $x genetika $7 D014210
650    _2
$a sekvenování celého genomu $7 D000073336
651    _2
$a Čína $7 D002681
655    _2
$a časopisecké články $7 D016428
655    _2
$a Research Support, N.I.H., Extramural $7 D052061
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Šmajs, David $u Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
700    1_
$a Hu, Yongfei $u Dermatology Hospital, Southern Medical University, Guangzhou, China
700    1_
$a Ke, Wujian $u Dermatology Hospital, Southern Medical University, Guangzhou, China
700    1_
$a Pospíšilová, Petra $u Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
700    1_
$a Hawley, Kelly L $u Division of Infectious Diseases and Department of Pediatrics, Connecticut Children's Medical Center, Hartford, Connecticut, USA
700    1_
$a Caimano, Melissa J $u Departments of Medicine, Pediatrics, and Molecular Biology and Biophysics, UConn Health, Farmington, Connecticut, USA
700    1_
$a Radolf, Justin D $u Departments of Medicine, Pediatrics, and Molecular Biology and Biophysics, UConn Health, Farmington, Connecticut, USA $u Departments of Genetics and Genome Sciences, and Immunology, UConn Health, Farmington, Connecticut, USA
700    1_
$a Sena, Arlene $u Division of Infectious Diseases, Department of Medicine, University of North Carolina, Chapel Hill, North Carolina, USA
700    1_
$a Tucker, Joseph D $u Division of Infectious Diseases, Department of Medicine, University of North Carolina, Chapel Hill, North Carolina, USA $u Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
700    1_
$a Yang, Bin $u Dermatology Hospital, Southern Medical University, Guangzhou, China
700    1_
$a Juliano, Jonathan J $u Division of Infectious Diseases, Department of Medicine, University of North Carolina, Chapel Hill, North Carolina, USA $u Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina, USA $u Curriculum in Genetics and Molecular Biology, School of Medicine, University of North Carolina, Chapel Hill, North Carolina, USA
700    1_
$a Zheng, Heping $u Dermatology Hospital, Southern Medical University, Guangzhou, China
700    1_
$a Parr, Jonathan B $u Division of Infectious Diseases, Department of Medicine, University of North Carolina, Chapel Hill, North Carolina, USA $u Curriculum in Genetics and Molecular Biology, School of Medicine, University of North Carolina, Chapel Hill, North Carolina, USA
773    0_
$w MED00002745 $t The Journal of infectious diseases $x 1537-6613 $g Roč. 223, č. 5 (2021), s. 848-853
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32710788 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220425 $b ABA008
991    __
$a 20220506130019 $b ABA008
999    __
$a ok $b bmc $g 1789913 $s 1163709
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 223 $c 5 $d 848-853 $e 20210303 $i 1537-6613 $m The Journal of infectious diseases $n J Infect Dis $x MED00002745
GRA    __
$a K24 AI134990 $p NIAID NIH HHS $2 United States
GRA    __
$a R01 AI026756 $p NIAID NIH HHS $2 United States
GRA    __
$a U19 AI144177 $p NIAID NIH HHS $2 United States
LZP    __
$a Pubmed-20220425

Najít záznam

Citační ukazatele

Možnosti archivace