-
Something wrong with this record ?
Association between interferon-gamma (IFN-γ) gene polymorphisms (+874A/T and +2109A/G), and susceptibility to hepatitis B viral infection (HBV)
Mahmoud F. Dondeti, Mohamed S. Abdelkhalek, Hosam El-Din M Elezawy, Walaa F. Alsanie, Bassem M. Raafat, Amira M. Gamal-Eldeen, Roba M. Talaat
Language English Country Czech Republic
Document type Research Support, Non-U.S. Gov't
- MeSH
- Genetic Predisposition to Disease MeSH
- Hepatitis B * genetics MeSH
- Interferon-gamma genetics MeSH
- Polymorphism, Single Nucleotide genetics MeSH
- Humans MeSH
- Hepatitis B virus MeSH
- Check Tag
- Humans MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
Background: Interferon-gamma (IFN-γ) is a chief proinflammatory cytokine with a significant role in the immune response against viral infections. Today there is increasing evidence about the association between individual genetic polymorphisms and cytokines in predicting HBV infection susceptibility. Aim: This study aimed to investigate the association between IFN-γ gene polymorphisms and susceptibility to hepatitis B viral infection (HBV), and the impact of these genetic polymorphisms on IFN-γ production. IFN-γ (+874A/T, rs2430561, and +2109A/G, rs1861494) was genotyped by single-stranded polymorphism-polymerase chain reaction (SSP-PCR) in 126 Egyptians with chronic HBV infection and in 100 healthy control subjects. The plasma levels of IFN-γ were measured by Enzyme-linked immunosorbent assay (ELISA). Results: Compared to the control subjects there was a slight increase in +874TT genotype frequency in HBV patients. However, no statistical significance in IFN-γ (+874A/T and +2109A/G) genotype/allele distribution was demonstrated, indicating the lack of association between these SNPs and susceptibility to HBV infection. In +2109A/G, only AG genotype was observed with a complete abrogation of GG and AA genotypes. Haplotypes between different loci on selected genes showed insignificant changes in their frequency in patients and control subjects. HBV patients had a significantly higher level of IFN-γ (P < 0.001) compared to controls. The maximum significant increase in IFN-γ production was observed in subjects harboring the +874TA genotype. Conclusions: As no association could be characterized between the polymorphism in IFN-γ (+874A/T and +2109A/G) and susceptibility to chronic HBV infection, our data support the concept that IFN-γ gene polymorphisms are not predictors of HBV susceptibility in this segment of the Egyptian population.
National Research Centre Genetic Engineering and Biotechnology Research Division Dokki Cairo Egypt
University of Sadat City Molecular Biology Department Sadat City Egypt
References provided by Crossref.org
Literatura
- 000
- 00000naa a2200000 a 4500
- 001
- bmc22013837
- 003
- CZ-PrNML
- 005
- 20221227152721.0
- 007
- ta
- 008
- 220523s2022 xr f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.32725/jab.2022.001 $2 doi
- 040 __
- $a ABA008 $d ABA008 $e AACR2 $b cze
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Dondeti, Mahmoud F. $u University of Sadat City (USC), Genetic Engineering and Biotechnology Research Institute (GEBRI), Molecular Biology Department, Sadat City, Egypt; National Research Centre (NRC), Genetic Engineering and Biotechnology Research Division, Dokki, Cairo, Egypt
- 245 10
- $a Association between interferon-gamma (IFN-γ) gene polymorphisms (+874A/T and +2109A/G), and susceptibility to hepatitis B viral infection (HBV) / $c Mahmoud F. Dondeti, Mohamed S. Abdelkhalek, Hosam El-Din M Elezawy, Walaa F. Alsanie, Bassem M. Raafat, Amira M. Gamal-Eldeen, Roba M. Talaat
- 504 __
- $a Literatura
- 520 9_
- $a Background: Interferon-gamma (IFN-γ) is a chief proinflammatory cytokine with a significant role in the immune response against viral infections. Today there is increasing evidence about the association between individual genetic polymorphisms and cytokines in predicting HBV infection susceptibility. Aim: This study aimed to investigate the association between IFN-γ gene polymorphisms and susceptibility to hepatitis B viral infection (HBV), and the impact of these genetic polymorphisms on IFN-γ production. IFN-γ (+874A/T, rs2430561, and +2109A/G, rs1861494) was genotyped by single-stranded polymorphism-polymerase chain reaction (SSP-PCR) in 126 Egyptians with chronic HBV infection and in 100 healthy control subjects. The plasma levels of IFN-γ were measured by Enzyme-linked immunosorbent assay (ELISA). Results: Compared to the control subjects there was a slight increase in +874TT genotype frequency in HBV patients. However, no statistical significance in IFN-γ (+874A/T and +2109A/G) genotype/allele distribution was demonstrated, indicating the lack of association between these SNPs and susceptibility to HBV infection. In +2109A/G, only AG genotype was observed with a complete abrogation of GG and AA genotypes. Haplotypes between different loci on selected genes showed insignificant changes in their frequency in patients and control subjects. HBV patients had a significantly higher level of IFN-γ (P < 0.001) compared to controls. The maximum significant increase in IFN-γ production was observed in subjects harboring the +874TA genotype. Conclusions: As no association could be characterized between the polymorphism in IFN-γ (+874A/T and +2109A/G) and susceptibility to chronic HBV infection, our data support the concept that IFN-γ gene polymorphisms are not predictors of HBV susceptibility in this segment of the Egyptian population.
- 650 _7
- $a lidé $7 D006801 $2 czmesh
- 650 _7
- $a genetická predispozice k nemoci $7 D020022 $2 czmesh
- 650 _7
- $a virus hepatitidy B $7 D006515 $2 czmesh
- 650 17
- $a hepatitida B $x genetika $7 D006509 $2 czmesh
- 650 _7
- $a interferon gama $x genetika $7 D007371 $2 czmesh
- 650 _7
- $a jednonukleotidový polymorfismus $x genetika $7 D020641 $2 czmesh
- 655 _7
- $a práce podpořená grantem $7 D013485 $2 czmesh
- 700 1_
- $a Abdelkhalek, Mohamed S. $u University of Sadat City (USC), Genetic Engineering and Biotechnology Research Institute (GEBRI), Molecular Biology Department, Sadat City, Egypt
- 700 1_
- $a Elezawy, Hosam El-Din M . $u Menoufia University, National Liver Institute (NLI), Clinical Biochemistry and Molecular Diagnostics Department, Menoufia, Egypt
- 700 1_
- $a Alsanie, Walaa F. $u Taif University, College of Applied Medical Sciences, Clinical Laboratory Sciences Department, Taif, Saudi Arabia
- 700 1_
- $a Raafat, Bassem M. $u Taif University, College of Applied Medical Sciences, Radiological Sciences Department, Taif, Saudi Arabia
- 700 1_
- $a Gamal-Eldeen, Amira M. $u Taif University, College of Applied Medical Sciences, Clinical Laboratory Sciences Department, Taif, Saudi Arabia
- 700 1_
- $a Talaat, Roba M. $u University of Sadat City (USC), Genetic Engineering and Biotechnology Research Institute (GEBRI), Molecular Biology Department, Sadat City, Egypt
- 773 0_
- $t Journal of applied biomedicine $x 1214-021X $g Roč. 20, č. 1 (2022), s. 37-43 $w MED00012667
- 856 41
- $u https://jab.zsf.jcu.cz/ $y stránka časopisu
- 910 __
- $a ABA008 $b B 2301 $c 1249 $y p $z 0
- 990 __
- $a 20220523121448 $b ABA008
- 991 __
- $a 20221227152718 $b ABA008
- 999 __
- $a ok $b bmc $g 1795771 $s 1165052
- BAS __
- $a 3
- BMC __
- $a 2022 $b 20 $c 1 $d 37-43 $i 1214-021X $m Journal of Applied Biomedicine $x MED00012667
- LZP __
- $c NLK109 $d 20221227 $a NLK 2022-22/dk
