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Euglena gracilis can grow in the mixed culture containing Cladosporium westerdijkiae, Lysinibacillus boronitolerans and Pseudobacillus badius without the addition of vitamins B1 and B12

A. Lukáčová, T. Beck, L. Koptašiková, A. Benda, L. Tomečková, M. Trniková, D. Lihanová, JM. Steiner, J. Krajčovič, M. Vesteg

. 2022 ; 351 (-) : 50-59. [pub] 20220429

Language English Country Netherlands

Document type Journal Article

Euglena gracilis is a freshwater flagellate possessing secondary chloroplast of green algal origin. This protist has numerous biotechnological applications such as production of biofuels and pharmaceuticals, and it can be also used for bioremediation of polluted water and wastewater. One of the highest limitations for its large-scale cultivation is that it cannot synthesize vitamins B1 and B12 which are expensive and they have to be added to media. This study revealed that E. gracilis can be grown for long time periods without the addition of vitamins B1 and B12 in the co-culture containing filamentous fungus Cladosporium westerdijkiae, and bacteria Lysinibacillus boronitolerans and Pseudobacillus badius. Growing of E. gracilis in such co-cultures without the addition of vitamins can dramatically reduce large scale cultivation costs. Moreover, C. westerdijkiae could be used in biotechnology for immobilization and effective harvesting of E. gracilis from big cultivation containers by bioflocculation.

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$a Lukáčová, Alexandra $u Department of Biology and Ecology, Faculty of Natural Sciences, Matej Bel University, 974 01 Banská Bystrica, Slovakia
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$a Euglena gracilis is a freshwater flagellate possessing secondary chloroplast of green algal origin. This protist has numerous biotechnological applications such as production of biofuels and pharmaceuticals, and it can be also used for bioremediation of polluted water and wastewater. One of the highest limitations for its large-scale cultivation is that it cannot synthesize vitamins B1 and B12 which are expensive and they have to be added to media. This study revealed that E. gracilis can be grown for long time periods without the addition of vitamins B1 and B12 in the co-culture containing filamentous fungus Cladosporium westerdijkiae, and bacteria Lysinibacillus boronitolerans and Pseudobacillus badius. Growing of E. gracilis in such co-cultures without the addition of vitamins can dramatically reduce large scale cultivation costs. Moreover, C. westerdijkiae could be used in biotechnology for immobilization and effective harvesting of E. gracilis from big cultivation containers by bioflocculation.
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$a Beck, Terézia $u Department of Biology and Ecology, Faculty of Natural Sciences, Matej Bel University, 974 01 Banská Bystrica, Slovakia
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$a Koptašiková, Lenka $u Imaging methods core facility at BIOCEV, Faculty of Science, Charles University, Vestec, Prague-West 252 50, Czech Republic
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$a Benda, Aleš $u Imaging methods core facility at BIOCEV, Faculty of Science, Charles University, Vestec, Prague-West 252 50, Czech Republic
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$a Tomečková, Lucia $u Department of Parasitology, BIOCEV, Faculty of Science, Charles University, Vestec, Prague-West 252 50, Czech Republic
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$a Trniková, Miriam $u Department of Biology and Ecology, Faculty of Natural Sciences, Matej Bel University, 974 01 Banská Bystrica, Slovakia
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$a Lihanová, Diana $u Department of Biology and Ecology, Faculty of Natural Sciences, Matej Bel University, 974 01 Banská Bystrica, Slovakia
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$a Steiner, Jürgen Michael $u Department of Biology and Ecology, Faculty of Natural Sciences, Matej Bel University, 974 01 Banská Bystrica, Slovakia
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$a Krajčovič, Juraj $u Department of Biology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, 917 01 Trnava, Slovakia
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$a Vesteg, Matej $u Department of Biology and Ecology, Faculty of Natural Sciences, Matej Bel University, 974 01 Banská Bystrica, Slovakia. Electronic address: matej.vesteg@umb.sk
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