• Je něco špatně v tomto záznamu ?

Glucosylated 5-Hydroxymethylpyrimidines as Epigenetic DNA Bases Regulating Transcription and Restriction Cleavage

A. Chakrapani, O. Ruiz-Larrabeiti, R. Pohl, M. Svoboda, L. Krásný, M. Hocek

. 2022 ; 28 (31) : e202200911. [pub] 20220413

Jazyk angličtina Země Německo

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc22018114

Grantová podpora
20-00885X Grantová Agentura České Republiky
22-12023S Grantová Agentura České Republiky
CZ.02.1.01/0.0/0.0/16_019/0000729 European Regional Development Fund

5-(β-d-Glucopyranosyloxymethyl)-2'-deoxyuridine and -cytidine 5'-O-triphosphates were prepared and used for polymerase-mediated (primer extension or PCR) synthesis of DNA containing glucosylated 5-hydroxymethyluracil (5hmU) or 5-hydroxymethyluracil (5hmC). The presence of any glucosylated pyrimidines fully protected DNA from cleavage by type II restriction endonucleases. On the other hand, while the presence of glucosylated 5hmU completely inhibited transcription by bacterial (Escherichia coli) RNA polymerase, the DNA containing the corresponding glucosylated 5hmC allowed a similar level of transcription as natural DNA. This suggests different roles of these hypermodified bases in the epigenetic regulation of transcription in bacteriophages or kinetoplastid parasites. Consequently, enzymatic glucosylation of 5hmC-containing DNA can be used for tuning of transcription activity.

000      
00000naa a2200000 a 4500
001      
bmc22018114
003      
CZ-PrNML
005      
20220804134551.0
007      
ta
008      
220720s2022 gw f 000 0|eng||
009      
AR
024    7_
$a 10.1002/chem.202200911 $2 doi
035    __
$a (PubMed)35355345
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a gw
100    1_
$a Chakrapani, Aswathi $u Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nam. 2, 16610, Prague 6, Czech Republic $u Department of Organic Chemistry, Faculty of Science, Charles University, Hlavova 8, CZ-12843, Prague 2, Czech Republic
245    10
$a Glucosylated 5-Hydroxymethylpyrimidines as Epigenetic DNA Bases Regulating Transcription and Restriction Cleavage / $c A. Chakrapani, O. Ruiz-Larrabeiti, R. Pohl, M. Svoboda, L. Krásný, M. Hocek
520    9_
$a 5-(β-d-Glucopyranosyloxymethyl)-2'-deoxyuridine and -cytidine 5'-O-triphosphates were prepared and used for polymerase-mediated (primer extension or PCR) synthesis of DNA containing glucosylated 5-hydroxymethyluracil (5hmU) or 5-hydroxymethyluracil (5hmC). The presence of any glucosylated pyrimidines fully protected DNA from cleavage by type II restriction endonucleases. On the other hand, while the presence of glucosylated 5hmU completely inhibited transcription by bacterial (Escherichia coli) RNA polymerase, the DNA containing the corresponding glucosylated 5hmC allowed a similar level of transcription as natural DNA. This suggests different roles of these hypermodified bases in the epigenetic regulation of transcription in bacteriophages or kinetoplastid parasites. Consequently, enzymatic glucosylation of 5hmC-containing DNA can be used for tuning of transcription activity.
650    12
$a DNA $7 D004247
650    _2
$a DNA řízené RNA-polymerasy $7 D012321
650    12
$a epigeneze genetická $7 D044127
650    _2
$a polymerázová řetězová reakce $7 D016133
655    _2
$a časopisecké články $7 D016428
700    1_
$a Ruiz-Larrabeiti, Olatz $u Dept. of Microbial Genetics and Gene Expression, Institute of Microbiology, Czech Academy of Sciences, 14220, Prague 4, Czech Republic
700    1_
$a Pohl, Radek $u Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nam. 2, 16610, Prague 6, Czech Republic
700    1_
$a Svoboda, Martin $u Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nam. 2, 16610, Prague 6, Czech Republic
700    1_
$a Krásný, Libor $u Dept. of Microbial Genetics and Gene Expression, Institute of Microbiology, Czech Academy of Sciences, 14220, Prague 4, Czech Republic
700    1_
$a Hocek, Michal $u Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nam. 2, 16610, Prague 6, Czech Republic $u Department of Organic Chemistry, Faculty of Science, Charles University, Hlavova 8, CZ-12843, Prague 2, Czech Republic $1 https://orcid.org/0000000211132047 $7 mzk2006368321
773    0_
$w MED00004916 $t Chemistry (Weinheim an der Bergstrasse, Germany) $x 1521-3765 $g Roč. 28, č. 31 (2022), s. e202200911
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35355345 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220720 $b ABA008
991    __
$a 20220804134545 $b ABA008
999    __
$a ok $b bmc $g 1821944 $s 1169357
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2022 $b 28 $c 31 $d e202200911 $e 20220413 $i 1521-3765 $m Chemistry $n Chemistry $x MED00004916
GRA    __
$a 20-00885X $p Grantová Agentura České Republiky
GRA    __
$a 22-12023S $p Grantová Agentura České Republiky
GRA    __
$a CZ.02.1.01/0.0/0.0/16_019/0000729 $p European Regional Development Fund
LZP    __
$a Pubmed-20220720

Najít záznam

Citační ukazatele

Možnosti archivace