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In vitro antiproliferative and cytotoxic activities of novel triphenyltin isoselenocyanate in human breast carcinoma cell lines MCF 7 and MDA-MB-231
L. Hunakova, E. Horvathova, M. Matuskova, P. Bobal, J. Otevrel, J. Brtko
Language English Country United States
Document type Journal Article
Grant support
APVV-15-0372
Agentúra na Podporu Výskumu a Vývoja
APVV-20-0314
Agentúra na Podporu Výskumu a Vývoja
VEGA 2/0126/19
Agentúra Ministerstva Školstva, Vedy, Výskumu a Športu SR
VEGA 1/0460/21
Agentúra Ministerstva Školstva, Vedy, Výskumu a Športu SR
VEGA 1/0136/18
Agentúra Ministerstva Školstva, Vedy, Výskumu a Športu SR
VEGA 1/0489/20
Agentúra Ministerstva Školstva, Vedy, Výskumu a Športu SR
VEGA 2/0116/21
Agentúra Ministerstva Školstva, Vedy, Výskumu a Športu SR
MUNI/A/1682/2020
Masarykova Univerzita
TRANSMED 2, ITMS: 26240120030
ERDF
TRANSMED, ITMS: 26240120008
ERDF
ITMS: 26240220071
ERDF
NLK
ProQuest Central
from 1997-03-01 to 1 year ago
Medline Complete (EBSCOhost)
from 2013-03-01 to 1 year ago
Health & Medicine (ProQuest)
from 1997-03-01 to 1 year ago
- MeSH
- Apoptosis MeSH
- Humans MeSH
- MCF-7 Cells MeSH
- Cell Line, Tumor MeSH
- Breast Neoplasms * drug therapy MeSH
- Organotin Compounds MeSH
- Organoselenium Compounds MeSH
- Cell Proliferation MeSH
- Antineoplastic Agents * chemistry pharmacology MeSH
- Selenium * pharmacology MeSH
- Triple Negative Breast Neoplasms * metabolism MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
Intensive investigation for novel antiproliferative and cytotoxic effective chemical compounds is currently concentrated on structurally modified agents of natural or synthetic source. The selenium derivative of triorganotin compound, triphenyltin isoselenocyanate (TPT-NCSe) caused higher cytotoxicity in hormone sensitive MCF 7 (IC 50-250 nM) in comparison with triple-negative MDA-MB-231 breast carcinoma cell line (IC 50-450 nM) as determined by MTT assay. Measurement of DNA damage showed presence of crosslinks in both cell lines treated by increasing TPT-NCSe concentrations. This compound decreased mitochondrial membrane potential shown by JC-1 staining in a concentration-dependent manner in both cell lines. Activation of caspases-3/7 was observed in MDA-MB-231 cells and was significant only by concentrations causing significant level of crosslinks. On the other hand, migration assay revealed inhibitory effect of viability keeping 100 nM concentration of TPT-NCSe on migration of MDA-MB-231 cells. Our data has shown that this selenium containing triorganotin molecule exerts DNA damage-linked antineoplastic activity in breast carcinoma cell lines studied.
References provided by Crossref.org
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