• Je něco špatně v tomto záznamu ?

A supernumerary "B-sex" chromosome drives male sex determination in the Pachón cavefish, Astyanax mexicanus

B. Imarazene, K. Du, S. Beille, E. Jouanno, R. Feron, Q. Pan, J. Torres-Paz, C. Lopez-Roques, A. Castinel, L. Gil, C. Kuchly, C. Donnadieu, H. Parrinello, L. Journot, C. Cabau, M. Zahm, C. Klopp, T. Pavlica, A. Al-Rikabi, T. Liehr, SA....

. 2021 ; 31 (21) : 4800-4809.e9. [pub] 20210907

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22019634

Grantová podpora
R01 GM085318 NIGMS NIH HHS - United States
R35 GM139635 NIGMS NIH HHS - United States

Sex chromosomes are generally derived from a pair of classical type-A chromosomes, and relatively few alternative models have been proposed up to now.1,2 B chromosomes (Bs) are supernumerary and dispensable chromosomes with non-Mendelian inheritance found in many plant and animal species3,4 that have often been considered as selfish genetic elements that behave as genome parasites.5,6 The observation that in some species Bs can be either restricted or predominant in one sex7-14 raised the interesting hypothesis that Bs could play a role in sex determination.15 The characterization of putative B master sex-determining (MSD) genes, however, has not yet been provided to support this hypothesis. Here, in Astyanax mexicanus cavefish originating from Pachón cave, we show that Bs are strongly male predominant. Based on a high-quality genome assembly of a B-carrying male, we characterized the Pachón cavefish B sequence and found that it contains two duplicated loci of the putative MSD gene growth differentiation factor 6b (gdf6b). Supporting its role as an MSD gene, we found that the Pachón cavefish gdf6b gene is expressed specifically in differentiating male gonads, and that its knockout induces male-to-female sex reversal in B-carrying males. This demonstrates that gdf6b is necessary for triggering male sex determination in Pachón cavefish. Altogether these results bring multiple and independent lines of evidence supporting the conclusion that the Pachón cavefish B is a "B-sex" chromosome that contains duplicated copies of the gdf6b gene, which can promote male sex determination in this species.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22019634
003      
CZ-PrNML
005      
20220804135838.0
007      
ta
008      
220720s2021 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.cub.2021.08.030 $2 doi
035    __
$a (PubMed)34496222
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Imarazene, Boudjema $u INRAE, LPGP, 35000 Rennes, France; Université Paris-Saclay, CNRS, Institut des Neurosciences Paris-Saclay, 91198 Gif sur Yvette, France
245    12
$a A supernumerary "B-sex" chromosome drives male sex determination in the Pachón cavefish, Astyanax mexicanus / $c B. Imarazene, K. Du, S. Beille, E. Jouanno, R. Feron, Q. Pan, J. Torres-Paz, C. Lopez-Roques, A. Castinel, L. Gil, C. Kuchly, C. Donnadieu, H. Parrinello, L. Journot, C. Cabau, M. Zahm, C. Klopp, T. Pavlica, A. Al-Rikabi, T. Liehr, SA. Simanovsky, J. Bohlen, A. Sember, J. Perez, F. Veyrunes, TD. Mueller, JH. Postlethwait, M. Schartl, A. Herpin, S. Rétaux, Y. Guiguen
520    9_
$a Sex chromosomes are generally derived from a pair of classical type-A chromosomes, and relatively few alternative models have been proposed up to now.1,2 B chromosomes (Bs) are supernumerary and dispensable chromosomes with non-Mendelian inheritance found in many plant and animal species3,4 that have often been considered as selfish genetic elements that behave as genome parasites.5,6 The observation that in some species Bs can be either restricted or predominant in one sex7-14 raised the interesting hypothesis that Bs could play a role in sex determination.15 The characterization of putative B master sex-determining (MSD) genes, however, has not yet been provided to support this hypothesis. Here, in Astyanax mexicanus cavefish originating from Pachón cave, we show that Bs are strongly male predominant. Based on a high-quality genome assembly of a B-carrying male, we characterized the Pachón cavefish B sequence and found that it contains two duplicated loci of the putative MSD gene growth differentiation factor 6b (gdf6b). Supporting its role as an MSD gene, we found that the Pachón cavefish gdf6b gene is expressed specifically in differentiating male gonads, and that its knockout induces male-to-female sex reversal in B-carrying males. This demonstrates that gdf6b is necessary for triggering male sex determination in Pachón cavefish. Altogether these results bring multiple and independent lines of evidence supporting the conclusion that the Pachón cavefish B is a "B-sex" chromosome that contains duplicated copies of the gdf6b gene, which can promote male sex determination in this species.
650    _2
$a zvířata $7 D000818
650    _2
$a biologická evoluce $7 D005075
650    _2
$a jeskyně $7 D059552
650    12
$a Characidae $x genetika $7 D060126
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a pohlavní chromozomy $x genetika $7 D012730
655    _2
$a časopisecké články $7 D016428
655    _2
$a Research Support, N.I.H., Extramural $7 D052061
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Du, Kang $u Xiphophorus Genetic Stock Center, Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX 78666, USA
700    1_
$a Beille, Séverine $u INRAE, LPGP, 35000 Rennes, France
700    1_
$a Jouanno, Elodie $u INRAE, LPGP, 35000 Rennes, France
700    1_
$a Feron, Romain $u INRAE, LPGP, 35000 Rennes, France; Department of Ecology and Evolution, University of Lausanne, Lausanne, Switzerland; Swiss Institute of Bioinformatics, Lausanne, Switzerland
700    1_
$a Pan, Qiaowei $u INRAE, LPGP, 35000 Rennes, France; Department of Ecology and Evolution, University of Lausanne, Lausanne, Switzerland
700    1_
$a Torres-Paz, Jorge $u Université Paris-Saclay, CNRS, Institut des Neurosciences Paris-Saclay, 91198 Gif sur Yvette, France
700    1_
$a Lopez-Roques, Céline $u INRAE, GeT-PlaGe, Genotoul, 31326 Castanet-Tolosan, France
700    1_
$a Castinel, Adrien $u INRAE, GeT-PlaGe, Genotoul, 31326 Castanet-Tolosan, France
700    1_
$a Gil, Lisa $u INRAE, GeT-PlaGe, Genotoul, 31326 Castanet-Tolosan, France
700    1_
$a Kuchly, Claire $u INRAE, GeT-PlaGe, Genotoul, 31326 Castanet-Tolosan, France
700    1_
$a Donnadieu, Cécile $u INRAE, GeT-PlaGe, Genotoul, 31326 Castanet-Tolosan, France
700    1_
$a Parrinello, Hugues $u Institut de Génomique Fonctionnelle, IGF, CNRS, INSERM, Univ. Montpellier, F-34094 Montpellier, France
700    1_
$a Journot, Laurent $u Institut de Génomique Fonctionnelle, IGF, CNRS, INSERM, Univ. Montpellier, F-34094 Montpellier, France
700    1_
$a Cabau, Cédric $u SIGENAE, GenPhySE, Université de Toulouse, INRAE, ENVT, Castanet Tolosan, France
700    1_
$a Zahm, Margot $u SIGENAE, UMIAT, INRAE, Castanet Tolosan, France
700    1_
$a Klopp, Christophe $u SIGENAE, UMIAT, INRAE, Castanet Tolosan, France
700    1_
$a Pavlica, Tomáš $u Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Rumburská 89, 27721 Liběchov, Czech Republic; Department of Zoology, Faculty of Science, Charles University, Viničná 7, 12844 Prague, Czech Republic
700    1_
$a Al-Rikabi, Ahmed $u University Clinic Jena, Institute of Human Genetics, 07747 Jena, Germany
700    1_
$a Liehr, Thomas $u University Clinic Jena, Institute of Human Genetics, 07747 Jena, Germany
700    1_
$a Simanovsky, Sergey A $u Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russia
700    1_
$a Bohlen, Joerg $u Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Rumburská 89, 27721 Liběchov, Czech Republic
700    1_
$a Sember, Alexandr $u Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Rumburská 89, 27721 Liběchov, Czech Republic
700    1_
$a Perez, Julie $u Institut des Sciences de l'Evolution de Montpellier (ISEM), CNRS, Université de Montpellier, IRD, 34095 Montpellier, France
700    1_
$a Veyrunes, Frédéric $u Institut des Sciences de l'Evolution de Montpellier (ISEM), CNRS, Université de Montpellier, IRD, 34095 Montpellier, France
700    1_
$a Mueller, Thomas D $u Department of Plant Physiology and Biophysics, Julius-von-Sachs Institute of the University Wuerzburg, D-97082 Wuerzburg, Germany
700    1_
$a Postlethwait, John H $u Institute of Neuroscience, University of Oregon, Eugene, OR, USA
700    1_
$a Schartl, Manfred $u Xiphophorus Genetic Stock Center, Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX 78666, USA; Department of Developmental Biochemistry, University of Wuerzburg, D-97074 Wuerzburg, Germany
700    1_
$a Herpin, Amaury $u INRAE, LPGP, 35000 Rennes, France
700    1_
$a Rétaux, Sylvie $u Université Paris-Saclay, CNRS, Institut des Neurosciences Paris-Saclay, 91198 Gif sur Yvette, France
700    1_
$a Guiguen, Yann $u INRAE, LPGP, 35000 Rennes, France. Electronic address: yann.guiguen@inrae.fr
773    0_
$w MED00006482 $t Current biology : CB $x 1879-0445 $g Roč. 31, č. 21 (2021), s. 4800-4809.e9
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34496222 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220720 $b ABA008
991    __
$a 20220804135831 $b ABA008
999    __
$a ok $b bmc $g 1823013 $s 1170877
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 31 $c 21 $d 4800-4809.e9 $e 20210907 $i 1879-0445 $m Current biology $n Curr Biol $x MED00006482
GRA    __
$a R01 GM085318 $p NIGMS NIH HHS $2 United States
GRA    __
$a R35 GM139635 $p NIGMS NIH HHS $2 United States
LZP    __
$a Pubmed-20220720

Najít záznam

Citační ukazatele

Možnosti archivace