Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Species-Specific Molecular Barriers to SARS-CoV-2 Replication in Bat Cells

SM. Aicher, F. Streicher, M. Chazal, D. Planas, D. Luo, J. Buchrieser, M. Nemcova, V. Seidlova, J. Zukal, J. Serra-Cobo, D. Pontier, B. Pain, G. Zimmer, O. Schwartz, P. Roingeard, J. Pikula, L. Dacheux, N. Jouvenet

. 2022 ; 96 (14) : e0060822. [pub] 20220705

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc22025151
E-resources Online Full text

NLK Free Medical Journals from 1967 to 6 months ago
Freely Accessible Science Journals from 1967 to 6 months ago
PubMed Central from 1967 to 1 year ago
Europe PubMed Central from 1967 to 6 months ago
Open Access Digital Library from 1967-02-01
Open Access Digital Library from 1967-02-01

Links

PubMed 35862713
DOI 10.1128/jvi.00608-22
Knihovny.cz E-resources

Bats are natural reservoirs of numerous coronaviruses, including the potential ancestor of SARS-CoV-2. Knowledge concerning the interaction between coronaviruses and bat cells is sparse. We investigated the ability of primary cells from Rhinolophus and Myotis species, as well as of established and novel cell lines from Myotis myotis, Eptesicus serotinus, Tadarida brasiliensis, and Nyctalus noctula, to support SARS-CoV-2 replication. None of these cells were permissive to infection, not even the ones expressing detectable levels of angiotensin-converting enzyme 2 (ACE2), which serves as the viral receptor in many mammalian species. The resistance to infection was overcome by expression of human ACE2 (hACE2) in three cell lines, suggesting that the restriction to viral replication was due to a low expression of bat ACE2 (bACE2) or the absence of bACE2 binding in these cells. Infectious virions were produced but not released from hACE2-transduced M. myotis brain cells. E. serotinus brain cells and M. myotis nasal epithelial cells expressing hACE2 efficiently controlled viral replication, which correlated with a potent interferon response. Our data highlight the existence of species-specific and cell-specific molecular barriers to viral replication in bat cells. These novel chiropteran cellular models are valuable tools to investigate the evolutionary relationships between bats and coronaviruses. IMPORTANCE Bats are host ancestors of several viruses that cause serious disease in humans, as illustrated by the ongoing SARS-CoV-2 pandemic. Progress in investigating bat-virus interactions has been hampered by a limited number of available bat cellular models. We have generated primary cells and cell lines from several bat species that are relevant for coronavirus research. The various permissivities of the cells to SARS-CoV-2 infection offered the opportunity to uncover some species-specific molecular restrictions to viral replication. All bat cells exhibited a potent entry-dependent restriction. Once this block was overcome by overexpression of human ACE2, which serves at the viral receptor, two bat cell lines controlled well viral replication, which correlated with the inability of the virus to counteract antiviral responses. Other cells potently inhibited viral release. Our novel bat cellular models contribute to a better understanding of the molecular interplays between bat cells and viruses.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22025151
003      
CZ-PrNML
005      
20221031101304.0
007      
ta
008      
221017s2022 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1128/jvi.00608-22 $2 doi
035    __
$a (PubMed)35862713
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Aicher, Sophie-Marie $u Institut Pasteurgrid.428999.7, Université de Paris Cité, CNRS UMR 3569, Virus Sensing and Signaling Unit, Paris, France
245    10
$a Species-Specific Molecular Barriers to SARS-CoV-2 Replication in Bat Cells / $c SM. Aicher, F. Streicher, M. Chazal, D. Planas, D. Luo, J. Buchrieser, M. Nemcova, V. Seidlova, J. Zukal, J. Serra-Cobo, D. Pontier, B. Pain, G. Zimmer, O. Schwartz, P. Roingeard, J. Pikula, L. Dacheux, N. Jouvenet
520    9_
$a Bats are natural reservoirs of numerous coronaviruses, including the potential ancestor of SARS-CoV-2. Knowledge concerning the interaction between coronaviruses and bat cells is sparse. We investigated the ability of primary cells from Rhinolophus and Myotis species, as well as of established and novel cell lines from Myotis myotis, Eptesicus serotinus, Tadarida brasiliensis, and Nyctalus noctula, to support SARS-CoV-2 replication. None of these cells were permissive to infection, not even the ones expressing detectable levels of angiotensin-converting enzyme 2 (ACE2), which serves as the viral receptor in many mammalian species. The resistance to infection was overcome by expression of human ACE2 (hACE2) in three cell lines, suggesting that the restriction to viral replication was due to a low expression of bat ACE2 (bACE2) or the absence of bACE2 binding in these cells. Infectious virions were produced but not released from hACE2-transduced M. myotis brain cells. E. serotinus brain cells and M. myotis nasal epithelial cells expressing hACE2 efficiently controlled viral replication, which correlated with a potent interferon response. Our data highlight the existence of species-specific and cell-specific molecular barriers to viral replication in bat cells. These novel chiropteran cellular models are valuable tools to investigate the evolutionary relationships between bats and coronaviruses. IMPORTANCE Bats are host ancestors of several viruses that cause serious disease in humans, as illustrated by the ongoing SARS-CoV-2 pandemic. Progress in investigating bat-virus interactions has been hampered by a limited number of available bat cellular models. We have generated primary cells and cell lines from several bat species that are relevant for coronavirus research. The various permissivities of the cells to SARS-CoV-2 infection offered the opportunity to uncover some species-specific molecular restrictions to viral replication. All bat cells exhibited a potent entry-dependent restriction. Once this block was overcome by overexpression of human ACE2, which serves at the viral receptor, two bat cell lines controlled well viral replication, which correlated with the inability of the virus to counteract antiviral responses. Other cells potently inhibited viral release. Our novel bat cellular models contribute to a better understanding of the molecular interplays between bat cells and viruses.
650    _2
$a angiotensin-konvertující enzym 2 $x genetika $7 D000085962
650    _2
$a zvířata $7 D000818
650    12
$a Chiroptera $x virologie $7 D002685
650    _2
$a lidé $7 D006801
650    _2
$a virové receptory $x metabolismus $7 D011991
650    12
$a SARS-CoV-2 $x fyziologie $7 D000086402
650    _2
$a druhová specificita $7 D013045
650    _2
$a glykoprotein S, koronavirus $x metabolismus $7 D064370
650    12
$a replikace viru $7 D014779
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Streicher, Felix $u Institut Pasteurgrid.428999.7, Université de Paris Cité, CNRS UMR 3569, Virus Sensing and Signaling Unit, Paris, France
700    1_
$a Chazal, Maxime $u Institut Pasteurgrid.428999.7, Université de Paris Cité, CNRS UMR 3569, Virus Sensing and Signaling Unit, Paris, France
700    1_
$a Planas, Delphine $u Institut Pasteurgrid.428999.7, Université de Paris Cité, CNRS UMR 3569, Virus and Immunity Unit, Paris, France $u Vaccine Research Institute, Créteil, France
700    1_
$a Luo, Dongsheng $u Institut Pasteurgrid.428999.7, Université de Paris Cité, Lyssavirus Epidemiology and Neuropathology Unit, Paris, France
700    1_
$a Buchrieser, Julian $u Institut Pasteurgrid.428999.7, Université de Paris Cité, CNRS UMR 3569, Virus and Immunity Unit, Paris, France
700    1_
$a Nemcova, Monika $u Department of Ecology and Diseases of Zoo Animals, Game, Fish and Bees, University of Veterinary Sciences Brno, Brno, Czech Republic
700    1_
$a Seidlova, Veronika $u Department of Ecology and Diseases of Zoo Animals, Game, Fish and Bees, University of Veterinary Sciences Brno, Brno, Czech Republic
700    1_
$a Zukal, Jan $u Institute of Vertebrate Biology of the Czech Academy of Sciences Brno, Brno, Czech Republic
700    1_
$a Serra-Cobo, Jordi $u Institut de Recerca de la Biodiversitat (IRBio), Faculty of Biology, Universitat de Barcelona, Barcelona, Spain $u Departament de Biologia Evolutiva, Ecologia i Ciències Ambientals, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain
700    1_
$a Pontier, Dominique $u Université de Lyon, LabEx Ecofect, Lyon, France $u Université Lyon 1, CNRS, Laboratoire de Biométrie et Biologie Evolutive UMR5558, Villeurbanne, France
700    1_
$a Pain, Bertrand $u University of Lyon, Université Lyon 1, INSERM, INRAE, Stem Cell and Brain Research Institute, Bron, France
700    1_
$a Zimmer, Gert $u Institute of Virology and Immunology, Bern & Mittelhäusern, Switzerland $u Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland $1 https://orcid.org/0000000227082507
700    1_
$a Schwartz, Olivier $u Institut Pasteurgrid.428999.7, Université de Paris Cité, CNRS UMR 3569, Virus and Immunity Unit, Paris, France $u Vaccine Research Institute, Créteil, France
700    1_
$a Roingeard, Philippe $u INSERM U1259 MAVIVH and Plateforme IBiSA de Microscopie Electronique, Faculté de Médecine, Université de Tours, Tours, France
700    1_
$a Pikula, Jiri $u Department of Ecology and Diseases of Zoo Animals, Game, Fish and Bees, University of Veterinary Sciences Brno, Brno, Czech Republic
700    1_
$a Dacheux, Laurent $u Institut Pasteurgrid.428999.7, Université de Paris Cité, Lyssavirus Epidemiology and Neuropathology Unit, Paris, France $1 https://orcid.org/0000000244013706
700    1_
$a Jouvenet, Nolwenn $u Institut Pasteurgrid.428999.7, Université de Paris Cité, CNRS UMR 3569, Virus Sensing and Signaling Unit, Paris, France $1 https://orcid.org/0000000161036048
773    0_
$w MED00003048 $t Journal of virology $x 1098-5514 $g Roč. 96, č. 14 (2022), s. e0060822
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35862713 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20221017 $b ABA008
991    __
$a 20221031101302 $b ABA008
999    __
$a ok $b bmc $g 1854716 $s 1176441
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2022 $b 96 $c 14 $d e0060822 $e 20220705 $i 1098-5514 $m Journal of virology $n J Virol $x MED00003048
LZP    __
$a Pubmed-20221017

Find record

Citation metrics

Archiving options