-
Je něco špatně v tomto záznamu ?
An environment-benign approach of bamboo pulp bleaching using extracellular xylanase of strain Bacillus stratosphericus EB-11 isolated from elephant dung
RK. Sarma, A. Gohain, TH. Ahmed, A. Yadav, R. Saikia
Jazyk angličtina Země Česko
Typ dokumentu časopisecké články
- MeSH
- Bacillus * MeSH
- endo-1,4-beta-xylanasy MeSH
- lignin chemie MeSH
- peroxid vodíku MeSH
- sloni * MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
The use of microbial enzymes is highly encouraged in paper and pulp industries to reduce the excessive use of hazardous chemicals. During the study, xylanase of Bacillus stratosphericus EB-11 was characterized for pulp bleaching applications. The extracellular xylanase was produced under submerged fermentation using bamboo waste as a natural carbon source. There was fast cell division and enzyme production under optimized fermentation conditions in the bioreactor. The highest activity was 91,200U after 30 h of growth with Km and Vmax of 3.52 mg/mL and 391.5 μmol/min per mg respectively. The purified enzyme with molecular mass ~ 60 kDa had conferred positive activity on native PAGE. The strong inhibition by ethylenediaminetetraacetate and SDS showed the metallo-xylanase nature of the purified enzyme. The bacterial xylanase reduces the use of hydrogen peroxide by 0.4%. Similarly, biological oxygen demand and chemical oxygen demand were reduced by 42.6 and 35.2%. The xylanase-hydrogen peroxide combined treatment and conventional chlorine dioxide-alkaline (CDE1D1D2) bleaching showed almost similar improvement in physicochemical properties of bamboo pulp. Xylanase-peroxide bleaching reduces the lignin content to 4.95% from 13.32% unbleached pulp. This content after CDE1D1D2 treatment was 4.21%. The kappa number decreased from 15.2 to 9.46 with increasing the burst factor (15.51), crystallinity index (60.25%), viscosity (20.1 cp), and brightness (65.4%). The overall finding will encourage the development of new cleaner methods of bleaching in the paper and pulp industry.
Department of Botany Arunachal University of Studies PIN 792013 Namsai India
Department of Botany SBMS College Sualkuchi 781103 Assam India
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc23000894
- 003
- CZ-PrNML
- 005
- 20230307110355.0
- 007
- ta
- 008
- 230307s2023 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/s12223-022-01003-1 $2 doi
- 035 __
- $a (PubMed)36048323
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Sarma, Rupak Kumar $u Department of Botany, SBMS College, Sualkuchi-781103, Assam, India. rsarmaonline@gmail.com $1 https://orcid.org/0000000331013552
- 245 13
- $a An environment-benign approach of bamboo pulp bleaching using extracellular xylanase of strain Bacillus stratosphericus EB-11 isolated from elephant dung / $c RK. Sarma, A. Gohain, TH. Ahmed, A. Yadav, R. Saikia
- 520 9_
- $a The use of microbial enzymes is highly encouraged in paper and pulp industries to reduce the excessive use of hazardous chemicals. During the study, xylanase of Bacillus stratosphericus EB-11 was characterized for pulp bleaching applications. The extracellular xylanase was produced under submerged fermentation using bamboo waste as a natural carbon source. There was fast cell division and enzyme production under optimized fermentation conditions in the bioreactor. The highest activity was 91,200U after 30 h of growth with Km and Vmax of 3.52 mg/mL and 391.5 μmol/min per mg respectively. The purified enzyme with molecular mass ~ 60 kDa had conferred positive activity on native PAGE. The strong inhibition by ethylenediaminetetraacetate and SDS showed the metallo-xylanase nature of the purified enzyme. The bacterial xylanase reduces the use of hydrogen peroxide by 0.4%. Similarly, biological oxygen demand and chemical oxygen demand were reduced by 42.6 and 35.2%. The xylanase-hydrogen peroxide combined treatment and conventional chlorine dioxide-alkaline (CDE1D1D2) bleaching showed almost similar improvement in physicochemical properties of bamboo pulp. Xylanase-peroxide bleaching reduces the lignin content to 4.95% from 13.32% unbleached pulp. This content after CDE1D1D2 treatment was 4.21%. The kappa number decreased from 15.2 to 9.46 with increasing the burst factor (15.51), crystallinity index (60.25%), viscosity (20.1 cp), and brightness (65.4%). The overall finding will encourage the development of new cleaner methods of bleaching in the paper and pulp industry.
- 650 _2
- $a zvířata $7 D000818
- 650 12
- $a sloni $7 D004606
- 650 _2
- $a peroxid vodíku $7 D006861
- 650 12
- $a Bacillus $7 D001407
- 650 _2
- $a lignin $x chemie $7 D008031
- 650 _2
- $a endo-1,4-beta-xylanasy $7 D043364
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Gohain, Anwesha $u Department of Botany, Arunachal University of Studies, PIN-792013, Namsai, India
- 700 1_
- $a Ahmed, Tobiul Hussain $u Chemical Engineering Group, Engineering Sciences and Technology Division, CSIR-North East Institute of Science and Technology, Jorhat-785006, Assam, India
- 700 1_
- $a Yadav, Archana $u Biotechnology Group, Biological Sciences and Technology Division, CSIR-North East Institute of Science and Technology, Jorhat-785006, Assam, India
- 700 1_
- $a Saikia, Ratul $u Biotechnology Group, Biological Sciences and Technology Division, CSIR-North East Institute of Science and Technology, Jorhat-785006, Assam, India
- 773 0_
- $w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 68, č. 1 (2023), s. 135-149
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/36048323 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20230307 $b ABA008
- 991 __
- $a 20230307110350 $b ABA008
- 999 __
- $a ok $b bmc $g 1905263 $s 1187089
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2023 $b 68 $c 1 $d 135-149 $e 20220901 $i 1874-9356 $m Folia microbiologica $n Folia microbiol. (Prague) $x MED00011005
- LZP __
- $a Pubmed-20230307
