UNLABELLED: The aim of this study was to create a 3D printing material with bioactive properties that potentially could be used for a transparent removable orthodontic appliance. MATERIALS AND METHODS: To acrylic monomers, four bioactive glasses at 10% concentration were added, which release Ca, P, Si and F ions. The materials were printed on a 3D printer and tested for flexural strength (24 h and 30 days), sorption and solubility (7 days), ion release to artificial saliva pH = 4 and 7 (42 days) and cytotoxicity in the human fibroblast model. The released ions were determined by plasma spectrometry (Ca, P and Si ions) and ion-selective electrode (F measurement)s. RESULTS: The material obtained released Ca2+ and PO43- ions for a period of 42 days when using glass Biomin C at pH 4. The flexural strength depended on the direction in which the sample was printed relative to the 3D printer platform. Vertically printed samples had a resistance greater than 20%. The 10% Biomin C samples post-cured for 30 min with light had a survival rate of the cells after 72 h of 85%. CONCLUSIONS: Material for 3D printing with bioactive glass in its composition, which releases ions, can be used in the production of orthodontic aligners.

Department of Advanced Material Technologies Faculty of Chemistry Wroclaw University of Science and Technology Smoluchowskiego 25 50 372 Wroclaw Poland

Department of Dentofacial Orthopaedics and Orthodontics Division of Facial Abnormalities Wroclaw Medical University Krakowska 26 50 425 Wroclaw Poland

Department of Molecular and Cellular Biology Faculty of Pharmacy Wroclaw Medical University Borowska 211A 50 556 Wroclaw Poland

SpofaDental Markova 238 506 01 Jicin Czech Republic

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