-
Je něco špatně v tomto záznamu ?
Quartz crystal microbalance biosensor for the detection of procalcitonin
M. Pohanka
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
Odkazy
PubMed
36787686
DOI
10.1016/j.talanta.2023.124325
Knihovny.cz E-zdroje
- MeSH
- biosenzitivní techniky * metody MeSH
- imunoanalýza metody MeSH
- kovové nanočástice * chemie MeSH
- křemen MeSH
- mikrorovnovážné techniky křemenného krystalu metody MeSH
- prokalcitonin MeSH
- zlato chemie MeSH
- Publikační typ
- časopisecké články MeSH
Procalcitonin is a blood protein and precursor of the hormone calcitonin. The procalcitonin level increases due to bacterial infections, sepsis, and other related pathologies. Here, we present a simple biosensor for procalcitonin assay suitable for point-of-care tests as an alternative to the current laboratory methods. The biosensor was based on a QCM piezoelectric sensor and a conjugate of gold nanoparticles-antibodies conjugate. It was suitable for the procalcitonin assay in biological samples and fully correlated to the standard ELISA method, and it did not suffer false positive or negative results or interferences. The detection limit was equal to 37.8 ng/l and the quantification limit to 104 ng/l for a sample of 25 μl. The dynamic range of the assay was 37.8 ng/l to 30.0 μg/l. The practical relevance of the biosensor is expected considering the findings, and the possible application of the assay principle for the development of biosensors for other markers is inferred.
- 000
- 00000naa a2200000 a 4500
- 001
- bmc23003588
- 003
- CZ-PrNML
- 005
- 20230425140740.0
- 007
- ta
- 008
- 230418s2023 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.talanta.2023.124325 $2 doi
- 035 __
- $a (PubMed)36787686
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Pohanka, Miroslav $u Faculty of Military Health Sciences, University of Defense, Brno, Trebesska 1575, 50001 Hradec Kralove, Czech Republic. Electronic address: miroslav.pohanka@unob.cz
- 245 10
- $a Quartz crystal microbalance biosensor for the detection of procalcitonin / $c M. Pohanka
- 520 9_
- $a Procalcitonin is a blood protein and precursor of the hormone calcitonin. The procalcitonin level increases due to bacterial infections, sepsis, and other related pathologies. Here, we present a simple biosensor for procalcitonin assay suitable for point-of-care tests as an alternative to the current laboratory methods. The biosensor was based on a QCM piezoelectric sensor and a conjugate of gold nanoparticles-antibodies conjugate. It was suitable for the procalcitonin assay in biological samples and fully correlated to the standard ELISA method, and it did not suffer false positive or negative results or interferences. The detection limit was equal to 37.8 ng/l and the quantification limit to 104 ng/l for a sample of 25 μl. The dynamic range of the assay was 37.8 ng/l to 30.0 μg/l. The practical relevance of the biosensor is expected considering the findings, and the possible application of the assay principle for the development of biosensors for other markers is inferred.
- 650 12
- $a kovové nanočástice $x chemie $7 D053768
- 650 _2
- $a prokalcitonin $7 D000077740
- 650 _2
- $a mikrorovnovážné techniky křemenného krystalu $x metody $7 D058525
- 650 _2
- $a zlato $x chemie $7 D006046
- 650 12
- $a biosenzitivní techniky $x metody $7 D015374
- 650 _2
- $a imunoanalýza $x metody $7 D007118
- 650 _2
- $a křemen $7 D011791
- 655 _2
- $a časopisecké články $7 D016428
- 773 0_
- $w MED00004484 $t Talanta $x 1873-3573 $g Roč. 257, č. - (2023), s. 124325
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/36787686 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20230418 $b ABA008
- 991 __
- $a 20230425140736 $b ABA008
- 999 __
- $a ok $b bmc $g 1924336 $s 1189797
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2023 $b 257 $c - $d 124325 $e 20230203 $i 1873-3573 $m Talanta $n Talanta $x MED00004484
- LZP __
- $a Pubmed-20230418