-
Je něco špatně v tomto záznamu ?
A new electromembrane extraction probe for on-line connection with capillary electrophoresis for determination of substances in biological matrices
F. Opekar, P. Tůma
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
- MeSH
- elektroforéza kapilární * metody MeSH
- ethery * chemie MeSH
- kreatinin MeSH
- lidé MeSH
- membrány umělé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
A miniature probe for electromembrane extraction is developed and constructed. The tubular probe with an internal volume of 1.1 μL is made of polypropylene hollow fiber with a supported liquid membrane of 85% nitrophenyloctyl ether (NPOE) with 15% bis(2-ethylhexyl)phosphonic acid (DEHP). The probe is connected on-line to the electrophoresis with short separation capillary via an air assisted flow gating interface cast from poly (dimethylsiloxane). The compact instrument is computer controlled via LabView. The probe parameters are tested for extraction of creatinine and basic amino acids from artificial solution and human urine. The sensitivity of the electrophoretic determination after 300 s extraction at 150 V compared to the sensitivity without extraction is 4.9-fold and 2.6-fold higher for creatinine and arginine, respectively. The RSDs for peak area measured from 5 repeated extractions of 50 μM solutions are 7.5%, 7.2%, 8.6% and 9.2% for Crea, Lys, Arg and His, respectively. The probe can be used for all-day measurements. The preparation of the probe is simple and requires no special tool.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc23004026
- 003
- CZ-PrNML
- 005
- 20230425141038.0
- 007
- ta
- 008
- 230418s2023 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.talanta.2022.124149 $2 doi
- 035 __
- $a (PubMed)36463806
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Opekar, František $u Charles University, Faculty of Science, Department of Analytical Chemistry, Albertov 2030, 128 43, Prague 2, Czech Republic. Electronic address: frantisek.opekar@natur.cuni.cz
- 245 12
- $a A new electromembrane extraction probe for on-line connection with capillary electrophoresis for determination of substances in biological matrices / $c F. Opekar, P. Tůma
- 520 9_
- $a A miniature probe for electromembrane extraction is developed and constructed. The tubular probe with an internal volume of 1.1 μL is made of polypropylene hollow fiber with a supported liquid membrane of 85% nitrophenyloctyl ether (NPOE) with 15% bis(2-ethylhexyl)phosphonic acid (DEHP). The probe is connected on-line to the electrophoresis with short separation capillary via an air assisted flow gating interface cast from poly (dimethylsiloxane). The compact instrument is computer controlled via LabView. The probe parameters are tested for extraction of creatinine and basic amino acids from artificial solution and human urine. The sensitivity of the electrophoretic determination after 300 s extraction at 150 V compared to the sensitivity without extraction is 4.9-fold and 2.6-fold higher for creatinine and arginine, respectively. The RSDs for peak area measured from 5 repeated extractions of 50 μM solutions are 7.5%, 7.2%, 8.6% and 9.2% for Crea, Lys, Arg and His, respectively. The probe can be used for all-day measurements. The preparation of the probe is simple and requires no special tool.
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a kreatinin $7 D003404
- 650 12
- $a elektroforéza kapilární $x metody $7 D019075
- 650 12
- $a ethery $x chemie $7 D004987
- 650 _2
- $a membrány umělé $7 D008567
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Tůma, Petr $u Charles University, Third Faculty of Medicine, Department of Hygiene, Ruská 87, 100 00, Prague 10, Czech Republic. Electronic address: petr.tuma@lf3.cuni.cz
- 773 0_
- $w MED00004484 $t Talanta $x 1873-3573 $g Roč. 254, č. - (2023), s. 124149
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/36463806 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20230418 $b ABA008
- 991 __
- $a 20230425141035 $b ABA008
- 999 __
- $a ok $b bmc $g 1924592 $s 1190235
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2023 $b 254 $c - $d 124149 $e 20221129 $i 1873-3573 $m Talanta $n Talanta $x MED00004484
- LZP __
- $a Pubmed-20230418
