-
Something wrong with this record ?
Myh6 promoter-driven Cre recombinase excises floxed DNA fragments in a subset of male germline cells
C. Sheldon, CW. Kessinger, Y. Sun, MI. Kontaridis, Q. Ma, SS. Hammoud, Z. Gao, H. Zhang, Z. Lin
Language English Country England, Great Britain
Document type Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
Grant support
R01 HL146810
NHLBI NIH HHS - United States
R01 HL102368
NHLBI NIH HHS - United States
Links
PubMed
36584478
DOI
10.1016/j.yjmcc.2022.12.005
Knihovny.cz E-resources
- MeSH
- Integrases * genetics metabolism MeSH
- Mice, Transgenic MeSH
- Mice MeSH
- Promoter Regions, Genetic genetics MeSH
- Germ Cells * metabolism MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, N.I.H., Extramural MeSH
Myh6-Cre transgenic mouse line was known to express Cre recombinase only in the heart. Nevertheless, during breeding Myh6-Cre to Rosa26fstdTom reporter (tdTom) mouse line, we observed that a significant part of their F2 tdTom/+ offspring had tdTom reporter gene universally activated. Our results show that Myh6-Cre transgenic mice have Cre recombinase activity in a subpopulation of the male germline cells, and that Myh6 gene transcripts are enriched in the interstitial Leydig cells and the undifferentiated spermatogonia stem cells. In summary, the current study confirms that the previously known "heart-specific" Myh6 promoter drives Cre expression in the testis.
Cellular and Molecular Biology Program University of Michigan Ann Arbor MI USA
Department of Biological Chemistry and Molecular Pharmacology Harvard Medical School Boston MA USA
Department of Human Genetics University of Michigan Ann Arbor MI USA
Department of Obstetrics and Gynecology University of Michigan Ann Arbor MI USA
Department of Urology University of Michigan Ann Arbor MI USA
Masonic Medical Research Institute 2150 Bleecker Street Utica NY 13501 United States of America
- 000
- 00000naa a2200000 a 4500
- 001
- bmc23004323
- 003
- CZ-PrNML
- 005
- 20230425141305.0
- 007
- ta
- 008
- 230418s2023 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.yjmcc.2022.12.005 $2 doi
- 035 __
- $a (PubMed)36584478
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Sheldon, Caroline $u Masonic Medical Research Institute, 2150 Bleecker Street, Utica, NY 13501, United States of America
- 245 10
- $a Myh6 promoter-driven Cre recombinase excises floxed DNA fragments in a subset of male germline cells / $c C. Sheldon, CW. Kessinger, Y. Sun, MI. Kontaridis, Q. Ma, SS. Hammoud, Z. Gao, H. Zhang, Z. Lin
- 520 9_
- $a Myh6-Cre transgenic mouse line was known to express Cre recombinase only in the heart. Nevertheless, during breeding Myh6-Cre to Rosa26fstdTom reporter (tdTom) mouse line, we observed that a significant part of their F2 tdTom/+ offspring had tdTom reporter gene universally activated. Our results show that Myh6-Cre transgenic mice have Cre recombinase activity in a subpopulation of the male germline cells, and that Myh6 gene transcripts are enriched in the interstitial Leydig cells and the undifferentiated spermatogonia stem cells. In summary, the current study confirms that the previously known "heart-specific" Myh6 promoter drives Cre expression in the testis.
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a myši $7 D051379
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a promotorové oblasti (genetika) $x genetika $7 D011401
- 650 _2
- $a myši transgenní $7 D008822
- 650 12
- $a integrasy $x genetika $x metabolismus $7 D019426
- 650 12
- $a zárodečné buňky $x metabolismus $7 D005854
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a Research Support, N.I.H., Extramural $7 D052061
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Kessinger, Chase W $u Masonic Medical Research Institute, 2150 Bleecker Street, Utica, NY 13501, United States of America
- 700 1_
- $a Sun, Yan $u Masonic Medical Research Institute, 2150 Bleecker Street, Utica, NY 13501, United States of America
- 700 1_
- $a Kontaridis, Maria I $u Masonic Medical Research Institute, 2150 Bleecker Street, Utica, NY 13501, United States of America; Department of Medicine, Division of Cardiology, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA
- 700 1_
- $a Ma, Qianyi $u Department of Human Genetics, University of Michigan, Ann Arbor, MI, USA
- 700 1_
- $a Hammoud, Saher Sue $u Department of Human Genetics, University of Michigan, Ann Arbor, MI, USA; Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI, USA; Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI, USA; Department of Urology, University of Michigan, Ann Arbor, MI, USA
- 700 1_
- $a Gao, Zibei $u School of Life Science and Technology, ShanghaiTech University, 393 Middle Huaxia Road, Pudong, Shanghai 201210, China
- 700 1_
- $a Zhang, Hui $u School of Life Science and Technology, ShanghaiTech University, 393 Middle Huaxia Road, Pudong, Shanghai 201210, China
- 700 1_
- $a Lin, Zhiqiang $u Masonic Medical Research Institute, 2150 Bleecker Street, Utica, NY 13501, United States of America. Electronic address: zlin@mmri.edu
- 773 0_
- $w MED00002807 $t Journal of molecular and cellular cardiology $x 1095-8584 $g Roč. 175, č. - (2023), s. 62-66
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/36584478 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20230418 $b ABA008
- 991 __
- $a 20230425141302 $b ABA008
- 999 __
- $a ok $b bmc $g 1924792 $s 1190532
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2023 $b 175 $c - $d 62-66 $e 20221228 $i 1095-8584 $m Journal of Molecular and Cellular Cardiology $n J Mol Cell Cardiol $x MED00002807
- GRA __
- $a R01 HL146810 $p NHLBI NIH HHS $2 United States
- GRA __
- $a R01 HL102368 $p NHLBI NIH HHS $2 United States
- LZP __
- $a Pubmed-20230418