Myh6-Cre transgenic mouse line was known to express Cre recombinase only in the heart. Nevertheless, during breeding Myh6-Cre to Rosa26fstdTom reporter (tdTom) mouse line, we observed that a significant part of their F2 tdTom/+ offspring had tdTom reporter gene universally activated. Our results show that Myh6-Cre transgenic mice have Cre recombinase activity in a subpopulation of the male germline cells, and that Myh6 gene transcripts are enriched in the interstitial Leydig cells and the undifferentiated spermatogonia stem cells. In summary, the current study confirms that the previously known "heart-specific" Myh6 promoter drives Cre expression in the testis.

Cellular and Molecular Biology Program University of Michigan Ann Arbor MI USA

Department of Biological Chemistry and Molecular Pharmacology Harvard Medical School Boston MA USA

Department of Human Genetics University of Michigan Ann Arbor MI USA

Department of Medicine Division of Cardiology Beth Israel Deaconess Medical Center Boston MA 02115 USA

Department of Obstetrics and Gynecology University of Michigan Ann Arbor MI USA

Department of Urology University of Michigan Ann Arbor MI USA

Masonic Medical Research Institute 2150 Bleecker Street Utica NY 13501 United States of America

School of Life Science and Technology ShanghaiTech University 393 Middle Huaxia Road Pudong Shanghai 201210 China

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$a Kessinger, Chase W $u Masonic Medical Research Institute, 2150 Bleecker Street, Utica, NY 13501, United States of America

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$a Sun, Yan $u Masonic Medical Research Institute, 2150 Bleecker Street, Utica, NY 13501, United States of America

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$a Kontaridis, Maria I $u Masonic Medical Research Institute, 2150 Bleecker Street, Utica, NY 13501, United States of America; Department of Medicine, Division of Cardiology, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA

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$a Ma, Qianyi $u Department of Human Genetics, University of Michigan, Ann Arbor, MI, USA

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$a Hammoud, Saher Sue $u Department of Human Genetics, University of Michigan, Ann Arbor, MI, USA; Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI, USA; Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI, USA; Department of Urology, University of Michigan, Ann Arbor, MI, USA

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$a Gao, Zibei $u School of Life Science and Technology, ShanghaiTech University, 393 Middle Huaxia Road, Pudong, Shanghai 201210, China

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$a Zhang, Hui $u School of Life Science and Technology, ShanghaiTech University, 393 Middle Huaxia Road, Pudong, Shanghai 201210, China

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$a Lin, Zhiqiang $u Masonic Medical Research Institute, 2150 Bleecker Street, Utica, NY 13501, United States of America. Electronic address: zlin@mmri.edu

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