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Quality of MALDI-TOF mass spectra in routine diagnostics: results from an international external quality assessment including 36 laboratories from 12 countries using 47 challenging bacterial strains
A. Cuénod, M. Aerni, C. Bagutti, B. Bayraktar, ES. Boz, CB. Carneiro, C. Casanova, AT. Coste, P. Damborg, DW. van Dam, M. Demirci, P. Drevinek, O. Dubuis, J. Fernandez, G. Greub, J. Hrabak, G. Hürkal Yiğitler, J. Hurych, TG. Jensen, G. Jost, GA....
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články
- MeSH
- Bacteria * MeSH
- laboratoře * MeSH
- lidé MeSH
- průběh práce MeSH
- reprodukovatelnost výsledků MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
OBJECTIVES: Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) is a widely used method for bacterial species identification. Incomplete databases and mass spectral quality (MSQ) still represent major challenges. Important proxies for MSQ are the number of detected marker masses, reproducibility, and measurement precision. We aimed to assess MSQs across diagnostic laboratories and the potential of simple workflow adaptations to improve it. METHODS: For baseline MSQ assessment, 47 diverse bacterial strains, which are challenging to identify by MALDI-TOF MS, were routinely measured in 36 laboratories from 12 countries, and well-defined MSQ features were used. After an intervention consisting of detailed reported feedback and instructions on how to acquire MALDI-TOF mass spectra, measurements were repeated and MSQs were compared. RESULTS: At baseline, we observed heterogeneous MSQ between the devices, considering the median number of marker masses detected (range = [2-25]), reproducibility between technical replicates (range = [55%-86%]), and measurement error (range = [147 parts per million (ppm)-588 ppm]). As a general trend, the spectral quality was improved after the intervention for devices, which yielded low MSQs in the baseline assessment as follows: for four out of five devices with a high measurement error, the measurement precision was improved (p-values <0.001, paired Wilcoxon test); for six out of ten devices, which detected a low number of marker masses, the number of detected marker masses increased (p-values <0.001, paired Wilcoxon test). DISCUSSION: We have identified simple workflow adaptations, which, to some extent, improve MSQ of poorly performing devices and should be considered by laboratories yielding a low MSQ. Improving MALDI-TOF MSQ in routine diagnostics is essential for increasing the resolution of bacterial identification by MALDI-TOF MS, which is dependent on the reproducible detection of marker masses. The heterogeneity identified in this external quality assessment (EQA) requires further study.
ADMED Microbiologie La Chaux de Fonds Switzerland
Applied Microbiology Research Department of Biomedicine University of Basel Basel Switzerland
Austrian Agency for Health and Food Safety Vienna Austria
Bioanalytica AG Lucerne Switzerland
Biomedical Center Faculty of Medicine in Pilsen Charles University Plzen Czech Republic
Cantonal Hospital Aarau Aarau Switzerland
Center for Laboratory Medicine St Gallen Switzerland
Clinical Laboratory AZNikolaas Sint Niklaas Belgium
Department of Clinical and Molecular Microbiology University Hospital Centre Zagreb Zagreb Croatia
Department of Clinical Microbiology Hvidovre Hospital Hvidovre Denmark
Department of Clinical Microbiology Odense University Hospital Odense Denmark
Department of Medical Microbiology Kirklareli University Kirklareli Turkey
Department of Medical Microbiology University of Antwerp Belgium
Department of Veterinary and Animal Sciences University of Copenhagen Frederiksberg Denmark
Division of Clinical Bacteriology and Mycology University Hospital Basel Basel Switzerland
Eurofins Scientific AG Schönenwerd Switzerland
Hospital General Universitario Gregorio Maranon Madrid Spain
Institute for Infectious Diseases University of Bern Bern Switzerland
Institute of Medical Microbiology and Hygiene University of Tübingen Tübingen Germany
Institute of Microbiology University Hospital Lausanne Lausanne Switzerland
Institute of Veterinary Bacteriology University of Bern Bern Switzerland
Labor Team W Goldach Switzerland
Mabritec AG Riehen Switzerland
MCL Laboratories Niederwangen Switzerland
Reference Services Division UK Health Security Agency London United Kingdom
State Laboratory Basel Stadt Basel Switzerland
UMC Utrecht Utrecht the Netherlands
University Hospital Freiburg Freiburg im Breisgau Germany
University Hospital of Wales Cardiff United Kingdom
University of Health Sciences Sisli Hamidiye Etfal Teaching and Research Hospital Istanbul Turkey
Citace poskytuje Crossref.org
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- $a Cuénod, Aline $u Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland; Division of Clinical Bacteriology and Mycology, University Hospital Basel, Basel, Switzerland. Electronic address: aline.cuenod@stud.unibas.ch
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- $a Quality of MALDI-TOF mass spectra in routine diagnostics: results from an international external quality assessment including 36 laboratories from 12 countries using 47 challenging bacterial strains / $c A. Cuénod, M. Aerni, C. Bagutti, B. Bayraktar, ES. Boz, CB. Carneiro, C. Casanova, AT. Coste, P. Damborg, DW. van Dam, M. Demirci, P. Drevinek, O. Dubuis, J. Fernandez, G. Greub, J. Hrabak, G. Hürkal Yiğitler, J. Hurych, TG. Jensen, G. Jost, GA. Kampinga, S. Kittl, C. Lammens, C. Lang, R. Lienhard, J. Logan, C. Maffioli, I. Mareković, M. Marschal, J. Moran-Gilad, O. Nolte, M. Oberle, M. Pedersen, V. Pflüger, S. Pranghofer, J. Reichl, RJ. Rentenaar, A. Riat, B. Rodríguez-Sánchez, C. Schilt, AK. Schlotterbeck, J. Schrenzel, S. Troib, E. Willems, M. Wootton, D. Ziegler, A. Egli, ESGMD study group
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