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[Anti-Restriction Activity of ArdB Protein against EcoAI Endonuclease]
AA. Kudryavtseva, VA. Alekhin, MD. Lebedeva, E. Cséfalvay, M. Weiserova, IV. Manukhov
Jazyk ruština Země Rusko
Typ dokumentu anglický abstrakt, časopisecké články
- MeSH
- DNA MeSH
- Escherichia coli * genetika metabolismus MeSH
- plazmidy genetika MeSH
- proteiny z Escherichia coli * genetika chemie MeSH
- restrikční enzymy genetika MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
ArdB proteins are known to inhibit the activity of the type I restriction-modification (RM-I) system, in particular EcoKI (IA family). The mechanism of ArdB's activity still remains unknown; the spectrum of targets inhibited has been poorly studied. In this work, it was shown that the presence of the ardB gene from the R64 plasmid could suppress the activity of EcoAI endonuclease (IB family) in Escherichia coli TG1 cells. Due to the absence of specificity of ArdB to a certain RM-I system (it inhibits both the IA- and IB-family), it can be assumed that the mechanism of the anti-restriction activity of this protein does not depend on the sequence DNA at the recognition site nor the structure of the restriction enzyme of the RM-I systems.
Moscow Institute of Physics and Technology Dolgoprudny Moscow oblast 141707 Russia
Moscow State University of Food Production Moscow 125080 Russia
Citace poskytuje Crossref.org
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