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Quantification of interaction frequency between antigen-presenting cells and T cells by conjugation assay
O. Cerny
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- aktivace lymfocytů MeSH
- antigen prezentující buňky * MeSH
- imunologické synapse fyziologie MeSH
- mikrofilamenta MeSH
- receptory antigenů T-buněk MeSH
- T-lymfocyty * MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Interaction between an antigen-presenting cell and a T cell, and their subsequent conjugation are a prerequisite for the formation of the immunological synapse and productive, antigen-dependent activation of T cells. This initial interaction is accompanied by recognition of the presented antigen by the T cell receptor, and by changes in the morphology of the interacting cells and in actin cytoskeleton structure in the site of interaction. The experimental protocol below describes a simple assay for quantitative assessment of antigen-presenting cells-T cell conjugation using confocal microscopy or flow cytometry.
Citace poskytuje Crossref.org
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