• Je něco špatně v tomto záznamu ?

Modulation of Cellular Senescence in HEK293 and HepG2 Cells by Ultrafiltrates UPla and ULu Is Partly Mediated by Modulation of Mitochondrial Homeostasis under Oxidative Stress

J. Zhou, K. Liu, C. Bauer, G. Bendner, H. Dietrich, JP. Slivka, M. Wink, MBF. Wong, MKS. Chan, T. Skutella

. 2023 ; 24 (7) : . [pub] 20230404

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc23010493

Grantová podpora
EUROPEAN WELLNESS ACADEMIE (ASIA PACIFIC) SDN. BHD
the Heidelberg University Clinic

Protein probes, including ultrafiltrates from the placenta (UPla) and lung (ULu) of postnatal rabbits, were investigated in premature senescent HEK293 and HepG2 cells to explore whether they could modulate cellular senescence. Tris-Tricine-PAGE, gene ontology (GO), and LC-MS/MS analysis were applied to describe the characteristics of the ultrafiltrates. HEK293 and HepG2 cells (both under 25 passages) exposed to a sub-toxic concentration of hydrogen peroxide (H2O2, 300 μM) became senescent; UPla (10 μg/mL), ULu (10 μg/mL), as well as positive controls lipoic acid (10 μg/mL) and transferrin (10 μg/mL) were added along with H2O2 to the cells. Cell morphology; cellular proliferation; senescence-associated beta-galactosidase (SA-β-X-gal) activity; expression of senescence biomarkers including p16 INK4A (p16), p21 Waf1/Cip1 (p21), HMGB1, MMP-3, TNF-α, IL-6, lamin B1, and phospho-histone H2A.X (γ-H2AX); senescence-related gene expression; reactive oxygen species (ROS) levels; and mitochondrial fission were examined. Tris-Tricine-PAGE revealed prominent detectable bands between 10 and 100 kDa. LC-MS/MS identified 150-180 proteins and peptides in the protein probes, and GO analysis demonstrated a distinct enrichment of proteins associated with "extracellular space" and "proteasome core complex". UPla and ULu modulated senescent cell morphology, improved cell proliferation, and decreased beta-galactosidase activity, intracellular and mitochondrial ROS production, and mitochondrial fission caused by H2O2. The results from this study demonstrated that UPla and Ulu, as well as lipoic acid and transferrin, could protect HEK293 and HepG2 cells from H2O2-induced oxidative damage via protecting mitochondrial homeostasis and thus have the potential to be explored in anti-aging therapies.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc23010493
003      
CZ-PrNML
005      
20230801132448.0
007      
ta
008      
230718s2023 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/ijms24076748 $2 doi
035    __
$a (PubMed)37047720
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Zhou, Junxian $u Institute for Anatomy and Cell Biology, Medical Faculty, Heidelberg University, 69120 Heidelberg, Germany
245    10
$a Modulation of Cellular Senescence in HEK293 and HepG2 Cells by Ultrafiltrates UPla and ULu Is Partly Mediated by Modulation of Mitochondrial Homeostasis under Oxidative Stress / $c J. Zhou, K. Liu, C. Bauer, G. Bendner, H. Dietrich, JP. Slivka, M. Wink, MBF. Wong, MKS. Chan, T. Skutella
520    9_
$a Protein probes, including ultrafiltrates from the placenta (UPla) and lung (ULu) of postnatal rabbits, were investigated in premature senescent HEK293 and HepG2 cells to explore whether they could modulate cellular senescence. Tris-Tricine-PAGE, gene ontology (GO), and LC-MS/MS analysis were applied to describe the characteristics of the ultrafiltrates. HEK293 and HepG2 cells (both under 25 passages) exposed to a sub-toxic concentration of hydrogen peroxide (H2O2, 300 μM) became senescent; UPla (10 μg/mL), ULu (10 μg/mL), as well as positive controls lipoic acid (10 μg/mL) and transferrin (10 μg/mL) were added along with H2O2 to the cells. Cell morphology; cellular proliferation; senescence-associated beta-galactosidase (SA-β-X-gal) activity; expression of senescence biomarkers including p16 INK4A (p16), p21 Waf1/Cip1 (p21), HMGB1, MMP-3, TNF-α, IL-6, lamin B1, and phospho-histone H2A.X (γ-H2AX); senescence-related gene expression; reactive oxygen species (ROS) levels; and mitochondrial fission were examined. Tris-Tricine-PAGE revealed prominent detectable bands between 10 and 100 kDa. LC-MS/MS identified 150-180 proteins and peptides in the protein probes, and GO analysis demonstrated a distinct enrichment of proteins associated with "extracellular space" and "proteasome core complex". UPla and ULu modulated senescent cell morphology, improved cell proliferation, and decreased beta-galactosidase activity, intracellular and mitochondrial ROS production, and mitochondrial fission caused by H2O2. The results from this study demonstrated that UPla and Ulu, as well as lipoic acid and transferrin, could protect HEK293 and HepG2 cells from H2O2-induced oxidative damage via protecting mitochondrial homeostasis and thus have the potential to be explored in anti-aging therapies.
650    _2
$a zvířata $7 D000818
650    _2
$a lidé $7 D006801
650    _2
$a králíci $7 D011817
650    _2
$a reaktivní formy kyslíku $x metabolismus $7 D017382
650    12
$a peroxid vodíku $x farmakologie $x metabolismus $7 D006861
650    _2
$a buňky Hep G2 $7 D056945
650    12
$a kyselina lipoová $x metabolismus $7 D008063
650    _2
$a chromatografie kapalinová $7 D002853
650    _2
$a HEK293 buňky $7 D057809
650    _2
$a tandemová hmotnostní spektrometrie $7 D053719
650    _2
$a oxidační stres $7 D018384
650    _2
$a stárnutí buněk $7 D016922
650    _2
$a beta-galaktosidasa $x metabolismus $7 D001616
650    _2
$a transferriny $x metabolismus $7 D061250
655    _2
$a časopisecké články $7 D016428
700    1_
$a Liu, Kang $u Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, China
700    1_
$a Bauer, Chris $u MicroDiscovery, 10405 Berlin, Germany
700    1_
$a Bendner, Gerald $u Institute for Anatomy and Cell Biology, Medical Faculty, Heidelberg University, 69120 Heidelberg, Germany $1 https://orcid.org/000900084989059X
700    1_
$a Dietrich, Heike $u Institute for Anatomy and Cell Biology, Medical Faculty, Heidelberg University, 69120 Heidelberg, Germany
700    1_
$a Slivka, Jakub Peter $u Reviva s.r.o. Pekarska 6, 158 00 Prague, Czech Republic
700    1_
$a Wink, Michael $u Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, 69120 Heidelberg, Germany $1 https://orcid.org/0000000278754510 $7 utb2011625860
700    1_
$a Wong, Michelle B F $u EW European Wellness International GmbH, 72184 Eutingen im Gäu, Germany
700    1_
$a Chan, Mike K S $u EW European Wellness International GmbH, 72184 Eutingen im Gäu, Germany
700    1_
$a Skutella, Thomas $u Institute for Anatomy and Cell Biology, Medical Faculty, Heidelberg University, 69120 Heidelberg, Germany $1 https://orcid.org/0000000348131213
773    0_
$w MED00176142 $t International journal of molecular sciences $x 1422-0067 $g Roč. 24, č. 7 (2023)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/37047720 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20230718 $b ABA008
991    __
$a 20230801132445 $b ABA008
999    __
$a ok $b bmc $g 1963113 $s 1196758
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2023 $b 24 $c 7 $e 20230404 $i 1422-0067 $m International journal of molecular sciences $n Int J Mol Sci $x MED00176142
GRA    __
$p EUROPEAN WELLNESS ACADEMIE (ASIA PACIFIC) SDN. BHD
GRA    __
$p the Heidelberg University Clinic
LZP    __
$a Pubmed-20230718

Najít záznam

Citační ukazatele

Možnosti archivace