-
Je něco špatně v tomto záznamu ?
A Complex Methodological Approach for the Screening of Efficient and Safe Cobalt Chelators
M. Moravcová, M. Hrubša, Z. Lomozová, MC. Catapano, R. Argento, E. Jirkovský, R. Kučera, L. Mercolini, P. Mladěnka
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
- MeSH
- chelátory * MeSH
- ionty MeSH
- kobalt * chemie MeSH
- oxychinolin MeSH
- Publikační typ
- časopisecké články MeSH
BACKGROUND: Cobalt is an essential trace element, but it can also rarely cause cobalt toxicity due to its release from cobalt-containing medical devices. Currently, there are no approved selective cobalt chelators, which would represent an optimal treatment modality. OBJECTIVE: This study aimed to develop a simple and complex methodological approach for screening potential cobalt chelators and evaluating their potential toxicity. METHODS: Firstly, a simple spectrophotometric assay employing 1-nitroso-2-naphthol-3,6- disulfonic acid disodium salt (NNDSA) for screening cobalt chelation was standardized at a pathophysiologically relevant range of pH 4.5-7.5. Then, the suitability of the method was verified using four known metal chelators (EDTA, 8-hydroxyquinoline, chloroxine and nitroxoline). As cobalt can catalyse the Fenton reaction, the potential toxicity of cobalt-chelator complexes was also determined by employing a novel HPLC method with coulometric detection. The effect on erythrocyte haemolysis was tested as well. RESULTS: The NNDSA method had high sensitivity enabling the detection of 25-200 nM of cobalt ions depending on pH conditions. Measurements could be carried out in a wide range of wavelengths from 470 to 540 nm. All tested complexes of the selected chelators decreased the rate of the Fenton reaction. Interestingly, chloroxine mixed with cobalt ions caused marked lysis of erythrocytes in contrast to the other compounds. CONCLUSION: The described complex methodological approach could serve as a simple yet precise tool for evaluating novel, effective and safe cobalt chelators.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc23010739
- 003
- CZ-PrNML
- 005
- 20230801132619.0
- 007
- ta
- 008
- 230718s2023 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.2174/1871530322666221006144449 $2 doi
- 035 __
- $a (PubMed)36201264
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Moravcová, Monika $u The Department of Pharmacology and Toxicology, Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, 50005 Hradec Králové, Czech Republic
- 245 12
- $a A Complex Methodological Approach for the Screening of Efficient and Safe Cobalt Chelators / $c M. Moravcová, M. Hrubša, Z. Lomozová, MC. Catapano, R. Argento, E. Jirkovský, R. Kučera, L. Mercolini, P. Mladěnka
- 520 9_
- $a BACKGROUND: Cobalt is an essential trace element, but it can also rarely cause cobalt toxicity due to its release from cobalt-containing medical devices. Currently, there are no approved selective cobalt chelators, which would represent an optimal treatment modality. OBJECTIVE: This study aimed to develop a simple and complex methodological approach for screening potential cobalt chelators and evaluating their potential toxicity. METHODS: Firstly, a simple spectrophotometric assay employing 1-nitroso-2-naphthol-3,6- disulfonic acid disodium salt (NNDSA) for screening cobalt chelation was standardized at a pathophysiologically relevant range of pH 4.5-7.5. Then, the suitability of the method was verified using four known metal chelators (EDTA, 8-hydroxyquinoline, chloroxine and nitroxoline). As cobalt can catalyse the Fenton reaction, the potential toxicity of cobalt-chelator complexes was also determined by employing a novel HPLC method with coulometric detection. The effect on erythrocyte haemolysis was tested as well. RESULTS: The NNDSA method had high sensitivity enabling the detection of 25-200 nM of cobalt ions depending on pH conditions. Measurements could be carried out in a wide range of wavelengths from 470 to 540 nm. All tested complexes of the selected chelators decreased the rate of the Fenton reaction. Interestingly, chloroxine mixed with cobalt ions caused marked lysis of erythrocytes in contrast to the other compounds. CONCLUSION: The described complex methodological approach could serve as a simple yet precise tool for evaluating novel, effective and safe cobalt chelators.
- 650 12
- $a chelátory $7 D002614
- 650 12
- $a kobalt $x chemie $7 D003035
- 650 _2
- $a ionty $7 D007477
- 650 _2
- $a oxychinolin $7 D015125
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Hrubša, Marcel $u The Department of Pharmacology and Toxicology, Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, 50005 Hradec Králové, Czech Republic
- 700 1_
- $a Lomozová, Zuzana $u The Department of Pharmacognosy, Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, 50005 Hradec Králové, Czech Republic
- 700 1_
- $a Catapano, Maria Carmen $u The Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, 50005 Hradec Králové, Czech Republic
- 700 1_
- $a Argento, Roberta $u The Department of Pharmacy and Biotechnology (FaBiT), Alma Mater Studiorum - The University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy
- 700 1_
- $a Jirkovský, Eduard $u The Department of Pharmacology and Toxicology, Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, 50005 Hradec Králové, Czech Republic
- 700 1_
- $a Kučera, Radim $u The Department of Pharmaceutical Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, 50005 Hradec Králové, Czech Republic
- 700 1_
- $a Mercolini, Laura $u The Department of Pharmacy and Biotechnology (FaBiT), Alma Mater Studiorum - The University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy
- 700 1_
- $a Mladěnka, Přemysl $u The Department of Pharmacology and Toxicology, Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, 50005 Hradec Králové, Czech Republic
- 773 0_
- $w MED00180387 $t Medicinal chemistry (Shariqah (United Arab Emirates)) $x 1875-6638 $g Roč. 19, č. 5 (2023), s. 495-507
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/36201264 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20230718 $b ABA008
- 991 __
- $a 20230801132615 $b ABA008
- 999 __
- $a ok $b bmc $g 1963284 $s 1197004
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2023 $b 19 $c 5 $d 495-507 $e - $i 1875-6638 $m Medicinal chemistry $n Med Chem $x MED00180387
- LZP __
- $a Pubmed-20230718
