Paratrimastix pyriformis is a free-living flagellate thriving in low-oxygen freshwater sediments. It belongs to the group Metamonada along with human parasites, such as Giardia and Trichomonas. Like other metamonads, P. pyriformis has a mitochondrion-related organelle (MRO) which in this protist is primarily involved in one-carbon folate metabolism. The MRO contains four members of the solute carrier family 25 (SLC25) responsible for the exchange of metabolites across the mitochondrial inner membrane. Here, we characterise the function of the adenine nucleotide carrier PpMC1 by thermostability shift and transport assays. We show that it transports ATP, ADP and, to a lesser extent, AMP, but not phosphate. The carrier is distinct in function and origin from both ADP/ATP carriers and ATP-Mg/phosphate carriers, and it most likely represents a distinct class of adenine nucleotide carriers.

Charles University Faculty of Science Department of Parasitology BIOCEV Vestec 252 50 Czech Republic

Medical Research Council Mitochondrial Biology Unit The Keith Peters Building Cambridge Biomedical Campus Hills Road Cambridge CB2 0XY United Kingdom

University of Stavanger Department of Chemistry Bioscience And Environmental Engineering Richard Johnsens Gate 4 N 4021 Stavanger Norway

Citace poskytuje Crossref.org