• Something wrong with this record ?

Unsolved mystery of Fas: mononuclear cells may have trouble dying in patients with Sjögren's syndrome

I. Lindrova, M. Kolackova, T. Svadlakova, R. Vankova, M. Chmelarova, M. Rosecka, E. Jozifkova, M. Sembera, J. Krejsek, R. Slezak

. 2023 ; 24 (1) : 12. [pub] 20230623

Language English Country England, Great Britain

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc23011004

BACKGROUND: Patients with Sjögren's syndrome, like other patients with autoimmune disorders, display dysregulation in the function of their immune system. Fas and Fas Ligand (FasL) are among the dysregulated proteins. METHODS: We studied Fas and FasL on IL-2Rα+ cells and in serum of patients with Sjögren's syndrome (n = 16) and healthy individuals (n = 16); both from same ethnic and geographical background. We used flow cytometry and enzyme-linked immunosorbent for this purpose. We also measured the expression of Bcl-2 and Bax by reverse transcription quantitative real-time PCR (RT-qPCR) and percentage of apoptotic and dead cells using Annexin V and 7-AAD staining in lymphocytes. RESULTS: FasL was increased in patients' T and B cells while Fas was increased in patients' monocytes, T and B cells. No signs of increased apoptosis were found. sFas and sFasL in patients' serum were increased, although the increase in sFasL was not significant. We suspect an effect of non-steroidal anti-inflammatory therapy on B cells, explaining the decrease of the percentage Fas+ B cells found within our samples. In healthy individuals, there was a noticeable pattern in the expression of FasL which mutually correlated to populations of mononuclear cells; this correlation was absent in the patients with Sjögren's syndrome. CONCLUSIONS: Mononuclear cells expressing IL-2Rα+ had upregulated Fas in Sjögren's syndrome. However, the rate of apoptosis based on Annexin V staining and the Bcl-2/Bax expression was not observed in mononuclear cells. We suspect a functional role of abnormal levels of Fas and FasL which has not been cleared yet.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc23011004
003      
CZ-PrNML
005      
20230801132743.0
007      
ta
008      
230718s2023 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1186/s12865-023-00544-5 $2 doi
035    __
$a (PubMed)37353767
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Lindrova, Irena $u Department of Dentistry, Faculty of Medicine in Hradec Kralove, Charles University and University Hospital, Sokolska 581, 500 05, Hradec Kralove, Czech Republic $1 https://orcid.org/0000000273273056
245    10
$a Unsolved mystery of Fas: mononuclear cells may have trouble dying in patients with Sjögren's syndrome / $c I. Lindrova, M. Kolackova, T. Svadlakova, R. Vankova, M. Chmelarova, M. Rosecka, E. Jozifkova, M. Sembera, J. Krejsek, R. Slezak
520    9_
$a BACKGROUND: Patients with Sjögren's syndrome, like other patients with autoimmune disorders, display dysregulation in the function of their immune system. Fas and Fas Ligand (FasL) are among the dysregulated proteins. METHODS: We studied Fas and FasL on IL-2Rα+ cells and in serum of patients with Sjögren's syndrome (n = 16) and healthy individuals (n = 16); both from same ethnic and geographical background. We used flow cytometry and enzyme-linked immunosorbent for this purpose. We also measured the expression of Bcl-2 and Bax by reverse transcription quantitative real-time PCR (RT-qPCR) and percentage of apoptotic and dead cells using Annexin V and 7-AAD staining in lymphocytes. RESULTS: FasL was increased in patients' T and B cells while Fas was increased in patients' monocytes, T and B cells. No signs of increased apoptosis were found. sFas and sFasL in patients' serum were increased, although the increase in sFasL was not significant. We suspect an effect of non-steroidal anti-inflammatory therapy on B cells, explaining the decrease of the percentage Fas+ B cells found within our samples. In healthy individuals, there was a noticeable pattern in the expression of FasL which mutually correlated to populations of mononuclear cells; this correlation was absent in the patients with Sjögren's syndrome. CONCLUSIONS: Mononuclear cells expressing IL-2Rα+ had upregulated Fas in Sjögren's syndrome. However, the rate of apoptosis based on Annexin V staining and the Bcl-2/Bax expression was not observed in mononuclear cells. We suspect a functional role of abnormal levels of Fas and FasL which has not been cleared yet.
650    _2
$a lidé $7 D006801
650    12
$a Sjögrenův syndrom $7 D012859
650    _2
$a annexin A5 $7 D017304
650    _2
$a receptor interleukinu-2 - alfa-podjednotka $x metabolismus $7 D053645
650    _2
$a protein X asociovaný s bcl-2 $x metabolismus $7 D051028
650    12
$a autoimunitní nemoci $7 D001327
650    _2
$a apoptóza $7 D017209
650    _2
$a antigeny CD95 $x metabolismus $7 D019014
655    _2
$a časopisecké články $7 D016428
700    1_
$a Kolackova, Martina $u Department of Clinical Immunology and Allergology, Faculty of Medicine in Hradec Kralove, Charles University, Simkova 870, 500 03, Hradec Kralove, Czech Republic. kolackovam@lfhk.cuni.cz $1 https://orcid.org/0000000315724565
700    1_
$a Svadlakova, Tereza $u Department of Clinical Immunology and Allergology, Faculty of Medicine in Hradec Kralove, Charles University, Simkova 870, 500 03, Hradec Kralove, Czech Republic $1 https://orcid.org/0000000215815531 $7 xx0268729
700    1_
$a Vankova, Radka $u Department of Clinical Immunology and Allergology, Faculty of Medicine in Hradec Kralove, Charles University, Simkova 870, 500 03, Hradec Kralove, Czech Republic $1 https://orcid.org/000000028503532X
700    1_
$a Chmelarova, Marcela $u Institute of Clinical Biochemistry and Diagnostics, University Hospital, Sokolska 581, 500 05, Hradec Kralove, Czech Republic
700    1_
$a Rosecka, Michaela $u Department of Clinical Immunology and Allergology, Faculty of Medicine in Hradec Kralove, Charles University, Simkova 870, 500 03, Hradec Kralove, Czech Republic $1 https://orcid.org/0000000325959990
700    1_
$a Jozifkova, Eva $u Department of Biology, Jan Evangelista Purkyne University, Za Valcovnou 1000/8, 400 96, Usti and Labem, Czech Republic $1 https://orcid.org/0000000333543469
700    1_
$a Sembera, Martin $u Department of Dentistry, Faculty of Medicine in Hradec Kralove, Charles University and University Hospital, Sokolska 581, 500 05, Hradec Kralove, Czech Republic $1 https://orcid.org/0000000232057090
700    1_
$a Krejsek, Jan $u Department of Clinical Immunology and Allergology, Faculty of Medicine in Hradec Kralove, Charles University, Simkova 870, 500 03, Hradec Kralove, Czech Republic $1 https://orcid.org/0000000327786800 $7 nlk19990073429
700    1_
$a Slezak, Radovan $u Department of Dentistry, Faculty of Medicine in Hradec Kralove, Charles University and University Hospital, Sokolska 581, 500 05, Hradec Kralove, Czech Republic $1 https://orcid.org/0000000249225062
773    0_
$w MED00008184 $t BMC immunology $x 1471-2172 $g Roč. 24, č. 1 (2023), s. 12
856    41
$u https://pubmed.ncbi.nlm.nih.gov/37353767 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20230718 $b ABA008
991    __
$a 20230801132740 $b ABA008
999    __
$a ok $b bmc $g 1963425 $s 1197269
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2023 $b 24 $c 1 $d 12 $e 20230623 $i 1471-2172 $m BMC immunology $n BMC Immunol $x MED00008184
LZP    __
$a Pubmed-20230718

Find record

Citation metrics

Archiving options