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RENEB Inter-Laboratory Comparison 2021: The Dicentric Chromosome Assay
D. Endesfelder, U. Oestreicher, M. Bucher, C. Beinke, C. Siebenwirth, E. Ainsbury, J. Moquet, G. Gruel, E. Gregoire, JS. Martinez, A. Vral, A. Baeyens, M. Valente, A. Montoro, G. Terzoudi, S. Triantopoulou, A. Pantelias, O. Monteiro Gil, MJ....
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Biological Assay methods MeSH
- Chromosome Aberrations * MeSH
- Chromosomes MeSH
- Humans MeSH
- Radiometry methods MeSH
- Retrospective Studies MeSH
- Radioactive Hazard Release * MeSH
- Dose-Response Relationship, Radiation MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
After large-scale radiation accidents where many individuals are suspected to be exposed to ionizing radiation, biological and physical retrospective dosimetry assays are important tools to aid clinical decision making by categorizing individuals into unexposed/minimally, moderately or highly exposed groups. Quality-controlled inter-laboratory comparisons of simulated accident scenarios are regularly performed in the frame of the European legal association RENEB (Running the European Network of Biological and Physical retrospective Dosimetry) to optimize international networking and emergency readiness in case of large-scale radiation events. In total 33 laboratories from 22 countries around the world participated in the current RENEB inter-laboratory comparison 2021 for the dicentric chromosome assay. Blood was irradiated in vitro with X rays (240 kVp, 13 mA, ∼75 keV, 1 Gy/min) to simulate an acute, homogeneous whole-body exposure. Three blood samples (no. 1: 0 Gy, no. 2: 1.2 Gy, no. 3: 3.5 Gy) were sent to each participant and the task was to culture samples, to prepare slides and to assess radiation doses based on the observed dicentric yields from 50 manually or 150 semi-automatically scored metaphases (triage mode scoring). Approximately two-thirds of the participants applied calibration curves from irradiations with γ rays and about 1/3 from irradiations with X rays with varying energies. The categorization of the samples in clinically relevant groups corresponding to individuals that were unexposed/minimally (0-1 Gy), moderately (1-2 Gy) or highly exposed (>2 Gy) was successfully performed by all participants for sample no. 1 and no. 3 and by ≥74% for sample no. 2. However, while most participants estimated a dose of exactly 0 Gy for the sham-irradiated sample, the precise dose estimates of the samples irradiated with doses >0 Gy were systematically higher than the corresponding reference doses and showed a median deviation of 0.5 Gy (sample no. 2) and 0.95 Gy (sample no. 3) for manual scoring. By converting doses estimated based on γ-ray calibration curves to X-ray doses of a comparable mean photon energy as used in this exercise, the median deviation decreased to 0.27 Gy (sample no. 2) and 0.6 Gy (sample no. 3). The main aim of biological dosimetry in the case of a large-scale event is the categorization of individuals into clinically relevant groups, to aid clinical decision making. This task was successfully performed by all participants for the 0 Gy and 3.5 Gy samples and by 74% (manual scoring) and 80% (semiautomatic scoring) for the 1.2 Gy sample. Due to the accuracy of the dicentric chromosome assay and the high number of participating laboratories, a systematic shift of the dose estimates could be revealed. Differences in radiation quality (X ray vs. γ ray) between the test samples and the applied dose effect curves can partly explain the systematic shift. There might be several additional reasons for the observed bias (e.g., donor effects, transport, experimental conditions or the irradiation setup) and the analysis of these reasons provides great opportunities for future research. The participation of laboratories from countries around the world gave the opportunity to compare the results on an international level.
Agenzia nazionale per le nuove tecnologie l'energia e lo sviluppo economico sostenibile Rome Italy
Biodosimetry Laboratory Center for Radiation Technology and Biotechnology
Bundesamt für Strahlenschutz BfS Oberschleissheim Germany
Bundeswehr Institute of Radiobiology Munich Germany
Consumer and Clinical Radiation Protection Bureau Health Canada Ottawa Canada
Cytogenetic Biodosimetry Laboratory
Dalat Nuclear Research Institute
Faculty of Medicine and Health Sciences Universiteit Gent Gent Belgium
Genevolution Porcheville France
Hospital General Universitario Gregorio Marañón
Institute of Nuclear Chemistry and Technology Warsaw Poland
Laboratori Nazionali di Legnaro Istituto Nazionale di Fisica Nucleare Legnaro Italy
Laboratorio de dosimetría biológica Madrid Spain
National Centre of Radiobiology and Radiation Protection Sofia Bulgaria
National Institutes for Quantum Science and Technology Chiba Japan
Nükleer Arş Ens Yarımburgaz mah Nükleer Arş yolu Turkey
Oak Ridge Associated Universities
Oak Ridge Institute for Science and Education
Radiation Cytogenetics Laboratory
Radiation Emergency Assistance Center Training Site
Radiation Protection Centre Department of Expertise and Exposure Monitoring Vilnius Lithuania
Serbian Institute of Occupational Health Belgrade Serbia
Servicio de Oncología Radioterápica
Universidad de Sevilla Departamento de Biología Celular Facultad de Biología Sevilla Spain
References provided by Crossref.org
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