Detail
Článek
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

DiaPASEF proteotype analysis indicates changes in cell growth and metabolic switch induced by caspase-9 inhibition in chondrogenic cells

P. Lapcik, B. Vesela, D. Potesil, K. Dadakova, M. Zapletalova, P. Benes, P. Bouchal, E. Matalova

. 2023 ; 23 (11) : e2200408. [pub] 20230407

Jazyk angličtina Země Německo

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc23011245

Caspase-9 is the major apical caspase responsible for triggering the intrinsic apoptotic pathway. Our previous study indicated that specific inhibition of caspase-9 caused microscopically evident alterations in appearance of the primary chondrogenic cultures which cannot be explained by decrease in apoptosis. To describe a complex molecular background of this effect, proteomics analysis of control and caspase-9 inhibitor-treated chondrogenic cultures were performed. Proteins were extracted, identified and quantified using LC-MS in both data dependent and data independent acquisition (DIA) mode. While directDIA analysis of diaPASEF data obtained using timsTOF Pro LC-MS system revealed 7849 protein groups (Q-value <0.01), a parallel analysis of iTRAQ-2DLC-MS3 and conventional DIA-MS data identified only 5146 and 4098 protein groups, respectively, showing diaPASEF a superior method for the study. The detailed analysis of diaPASEF data disclosed 236/551 significantly down-/up-regulated protein groups after caspase-9 inhibition, respectively (|log2FC|>0.58, Q value <0.05). Classification of downregulated proteins revealed changes in extracellular matrix organization, collagen metabolism, and muscle system processes. Moreover, deregulations suggest a switch from glycolytic to lipid based metabolism in the inhibited cells. No essential changes were found in the proteins involved in apoptosis. The data indicate new non-apoptotic participation of caspases in chondrocyte homeostasis with potential applications in cartilage pathophysiology.

000      
00000naa a2200000 a 4500
001      
bmc23011245
003      
CZ-PrNML
005      
20230801132914.0
007      
ta
008      
230718s2023 gw f 000 0|eng||
009      
AR
024    7_
$a 10.1002/pmic.202200408 $2 doi
035    __
$a (PubMed)36960851
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a gw
100    1_
$a Lapcik, Petr $u Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic
245    10
$a DiaPASEF proteotype analysis indicates changes in cell growth and metabolic switch induced by caspase-9 inhibition in chondrogenic cells / $c P. Lapcik, B. Vesela, D. Potesil, K. Dadakova, M. Zapletalova, P. Benes, P. Bouchal, E. Matalova
520    9_
$a Caspase-9 is the major apical caspase responsible for triggering the intrinsic apoptotic pathway. Our previous study indicated that specific inhibition of caspase-9 caused microscopically evident alterations in appearance of the primary chondrogenic cultures which cannot be explained by decrease in apoptosis. To describe a complex molecular background of this effect, proteomics analysis of control and caspase-9 inhibitor-treated chondrogenic cultures were performed. Proteins were extracted, identified and quantified using LC-MS in both data dependent and data independent acquisition (DIA) mode. While directDIA analysis of diaPASEF data obtained using timsTOF Pro LC-MS system revealed 7849 protein groups (Q-value <0.01), a parallel analysis of iTRAQ-2DLC-MS3 and conventional DIA-MS data identified only 5146 and 4098 protein groups, respectively, showing diaPASEF a superior method for the study. The detailed analysis of diaPASEF data disclosed 236/551 significantly down-/up-regulated protein groups after caspase-9 inhibition, respectively (|log2FC|>0.58, Q value <0.05). Classification of downregulated proteins revealed changes in extracellular matrix organization, collagen metabolism, and muscle system processes. Moreover, deregulations suggest a switch from glycolytic to lipid based metabolism in the inhibited cells. No essential changes were found in the proteins involved in apoptosis. The data indicate new non-apoptotic participation of caspases in chondrocyte homeostasis with potential applications in cartilage pathophysiology.
650    _2
$a kaspasa 9 $x metabolismus $x farmakologie $7 D053453
650    12
$a chondrocyty $x metabolismus $7 D019902
650    12
$a apoptóza $7 D017209
650    _2
$a signální transdukce $7 D015398
650    _2
$a kaspasy $x metabolismus $x farmakologie $7 D020169
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Vesela, Barbora $u Laboratory of Odontogenesis and Osteogenesis, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Brno, Czech Republic
700    1_
$a Potesil, David $u Proteomics core facility, Central European Institute for Technology, Masaryk University, Brno, Czech Republic
700    1_
$a Dadakova, Katerina $u Laboratory of Odontogenesis and Osteogenesis, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Brno, Czech Republic
700    1_
$a Zapletalova, Martina $u Laboratory of Odontogenesis and Osteogenesis, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Brno, Czech Republic
700    1_
$a Benes, Petr $u Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic $u International Clinical Research Center, St. Anne's University Hospital, Brno, Czech Republic
700    1_
$a Bouchal, Pavel $u Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic
700    1_
$a Matalova, Eva $u Laboratory of Odontogenesis and Osteogenesis, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Brno, Czech Republic $u Department of Physiology, Faculty of Veterinary Medicine, Veterinary University, Brno, Czech Republic
773    0_
$w MED00007044 $t Proteomics $x 1615-9861 $g Roč. 23, č. 11 (2023), s. e2200408
856    41
$u https://pubmed.ncbi.nlm.nih.gov/36960851 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20230718 $b ABA008
991    __
$a 20230801132911 $b ABA008
999    __
$a ok $b bmc $g 1963568 $s 1197510
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2023 $b 23 $c 11 $d e2200408 $e 20230407 $i 1615-9861 $m Proteomics $n Proteomics $x MED00007044
LZP    __
$a Pubmed-20230718

Najít záznam

Citační ukazatele

Možnosti archivace