• Something wrong with this record ?

The Effect of Vascular Endothelial Growth Factor C and Adipose-Derived Stem Cells on Lymphatic Regeneration in a Rat Vascularized Lymph Node Transfer Model

F. Jonas, P. Kesa, P. Paral, J. Pankrac, MH. Kalbacova, J. Miletin, A. Sukop, M. Molitor, K. Stanek, L. Sefc, O. Mestak

. 2023 ; 39 (4) : 311-319. [pub] 20220711

Language English Country United States

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc23011667

BACKGROUND: Lymphedema is a chronic condition characterized by progressive edema with complicated treatment. Recently, new treatment strategies inducing lymphangiogenesis were proposed. The aim of our study was to examine the effect of vascular endothelial growth factor C (VEGF-C) and adipose-derived stem cells (ADSCs) on lymphatic regeneration and drainage re-establishment in vascularized lymph node transfer (VLNT) model using a pedicled vascularized lymph node (VLN) groin flap. METHODS: Female Lewis rats with groin VLN flaps were utilized as a lymphedema model. Group A served as the control. Group B received VEGF-C. Group C received both VEGF-C and ADSCs. Group D received ADSCs only. Lymphatic drainage re-establishment was evaluated by ultrasound-photoacoustic imaging (US-PAI) after indocyanine green (ICG) injection. RESULTS: The fastest regeneration of elevated flaps was observed in Groups B and C in all monitored periods. After the first month, ICG positivity was detected in 14.3% of animals in Group A, 71.43% of animals in Group B (odds ratio [OR] = 15; p = 0.048), and 83.33% in Group C (OR = 30; p = 0.027). On the contrary, the difference between control group and Group D (16.67%; p = 0.905) was statistically insignificant. Administration of VEGF-C, ADSC + VEGF-C, and ADSC led to full flap regeneration after 6 months. The control group had the lowest percentage of ICG positivity at all monitored time points. CONCLUSION: We found that the fastest regeneration occurred with the combination of the VLN flap and VEGF-C. The addition of ADSC had an insignificant effect in our study. Furthermore, we proved the feasibility of PAI as an assessment tool of the lymphatic drainage recovery in a VLNT model.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc23011667
003      
CZ-PrNML
005      
20231005102612.0
007      
ta
008      
230718s2023 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1055/a-1896-5471 $2 doi
035    __
$a (PubMed)35817403
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Jonas, Filip $u Department of Plastic Surgery, University Hospital Bulovka, First Faculty of Medicine, Charles University, Prague, Czech Republic $u Department of Plastic Surgery, Third Faculty of Medicine, Teaching Hospital Královské Vinohrady, Charles University, Prague, Czech Republic
245    14
$a The Effect of Vascular Endothelial Growth Factor C and Adipose-Derived Stem Cells on Lymphatic Regeneration in a Rat Vascularized Lymph Node Transfer Model / $c F. Jonas, P. Kesa, P. Paral, J. Pankrac, MH. Kalbacova, J. Miletin, A. Sukop, M. Molitor, K. Stanek, L. Sefc, O. Mestak
520    9_
$a BACKGROUND: Lymphedema is a chronic condition characterized by progressive edema with complicated treatment. Recently, new treatment strategies inducing lymphangiogenesis were proposed. The aim of our study was to examine the effect of vascular endothelial growth factor C (VEGF-C) and adipose-derived stem cells (ADSCs) on lymphatic regeneration and drainage re-establishment in vascularized lymph node transfer (VLNT) model using a pedicled vascularized lymph node (VLN) groin flap. METHODS: Female Lewis rats with groin VLN flaps were utilized as a lymphedema model. Group A served as the control. Group B received VEGF-C. Group C received both VEGF-C and ADSCs. Group D received ADSCs only. Lymphatic drainage re-establishment was evaluated by ultrasound-photoacoustic imaging (US-PAI) after indocyanine green (ICG) injection. RESULTS: The fastest regeneration of elevated flaps was observed in Groups B and C in all monitored periods. After the first month, ICG positivity was detected in 14.3% of animals in Group A, 71.43% of animals in Group B (odds ratio [OR] = 15; p = 0.048), and 83.33% in Group C (OR = 30; p = 0.027). On the contrary, the difference between control group and Group D (16.67%; p = 0.905) was statistically insignificant. Administration of VEGF-C, ADSC + VEGF-C, and ADSC led to full flap regeneration after 6 months. The control group had the lowest percentage of ICG positivity at all monitored time points. CONCLUSION: We found that the fastest regeneration occurred with the combination of the VLN flap and VEGF-C. The addition of ADSC had an insignificant effect in our study. Furthermore, we proved the feasibility of PAI as an assessment tool of the lymphatic drainage recovery in a VLNT model.
650    _2
$a krysa rodu Rattus $7 D051381
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a zvířata $7 D000818
650    12
$a vaskulární endoteliální růstový faktor C $7 D042582
650    _2
$a potkani inbrední LEW $7 D011917
650    _2
$a lymfatické uzliny $x krevní zásobení $7 D008198
650    12
$a lymfedém $x chirurgie $x etiologie $7 D008209
650    _2
$a indokyanová zeleň $7 D007208
650    _2
$a regenerace $7 D012038
650    _2
$a kmenové buňky $7 D013234
655    _2
$a časopisecké články $7 D016428
700    1_
$a Kesa, Peter $u Center for Advanced Preclinical Imaging (CAPI), First Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Paral, Petr $u Center for Advanced Preclinical Imaging (CAPI), First Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Pankrac, Jan $u Center for Advanced Preclinical Imaging (CAPI), First Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Kalbacova, Marie Hubalek $u Institute of Pathological Physiology, First Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Miletin, Jakub $u Department of Plastic Surgery, Third Faculty of Medicine, Teaching Hospital Královské Vinohrady, Charles University, Prague, Czech Republic
700    1_
$a Sukop, Andrej $u Department of Plastic Surgery, Third Faculty of Medicine, Teaching Hospital Královské Vinohrady, Charles University, Prague, Czech Republic
700    1_
$a Molitor, Martin $u Department of Plastic Surgery, University Hospital Bulovka, First Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Staněk, Kryštof $u Department of Plastic Surgery, University Hospital Bulovka, First Faculty of Medicine, Charles University, Prague, Czech Republic $1 https://orcid.org/0000000329548849 $7 xx0307909
700    1_
$a Sefc, Ludek $u Center for Advanced Preclinical Imaging (CAPI), First Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Mestak, Ondrej $u Department of Plastic Surgery, University Hospital Bulovka, First Faculty of Medicine, Charles University, Prague, Czech Republic
773    0_
$w MED00186363 $t Journal of reconstructive microsurgery $x 1098-8947 $g Roč. 39, č. 4 (2023), s. 311-319
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35817403 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20230718 $b ABA008
991    __
$a 20231005102607 $b ABA008
999    __
$a ok $b bmc $g 1963860 $s 1197932
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2023 $b 39 $c 4 $d 311-319 $e 20220711 $i 1098-8947 $m Journal of reconstructive microsurgery $n J Reconstr Microsurg $x MED00186363
LZP    __
$a Pubmed-20230718

Find record

Citation metrics

Archiving options