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Bulk and single-nucleus RNA sequencing highlight immune pathways induced in individuals during an Ixodes scapularis tick bite

X. Tang, GE. Lynn, Y. Cui, J. Cerny, G. Arora, MM. Tomayko, J. Craft, E. Fikrig

. 2023 ; 91 (11) : e0028223. [pub] 20231017

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc24000663

Grantová podpora
126033 HHS | National Institutes of Health (NIH)
138949 HHS | National Institutes of Health (NIH)
52206 HHS | National Institutes of Health (NIH)
Steven and Alexandra Cohen Foundation (Steven & Alexandra Cohen Foundation)
Howard Hughes Medical Institute (HHMI)

E-zdroje Online Plný text

NLK Free Medical Journals od 1970 do Před 6 měsíci
Freely Accessible Science Journals od 1995 do Před 6 měsíci
PubMed Central od 1970 do Před 6 měsíci
Europe PubMed Central od 1970 do Před 6 měsíci
Open Access Digital Library od 1970-01-01
Open Access Digital Library od 1970-01-01

Odkazy

PubMed 37846980
DOI 10.1128/iai.00282-23
Knihovny.cz E-zdroje

Ticks are hematophagous arthropods that use a complex mixture of salivary proteins to evade host defenses while taking a blood meal. Little is known about the immunological and physiological consequences of tick feeding on humans. Here, we performed the first bulk and single-nucleus RNA sequencing (snRNA-seq) of skin and blood of four persons presenting with naturally acquired, attached Ixodes scapularis ticks. Pathways and individual genes associated with innate and adaptive immunity were identified based on bulk RNA sequencing, including interleukin-17 signaling and platelet activation pathways at the site of tick attachment or in peripheral blood. snRNA-seq further revealed that the Hippo signaling, cell adhesion, and axon guidance pathways were involved in the response to an I. scapularis bite in humans. Features of the host response in these individuals also overlapped with that of laboratory guinea pigs exposed to I. scapularis and which acquired resistance to ticks. These findings offer novel insights for the development of new biomarkers for I. scapularis exposure and anti-tick vaccines for human use.

Citace poskytuje Crossref.org

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