• Something wrong with this record ?

Bulk and single-nucleus RNA sequencing highlight immune pathways induced in individuals during an Ixodes scapularis tick bite

X. Tang, GE. Lynn, Y. Cui, J. Cerny, G. Arora, MM. Tomayko, J. Craft, E. Fikrig

. 2023 ; 91 (11) : e0028223. [pub] 20231017

Language English Country United States

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc24000663

Grant support
126033 HHS | National Institutes of Health (NIH)
138949 HHS | National Institutes of Health (NIH)
52206 HHS | National Institutes of Health (NIH)
Steven and Alexandra Cohen Foundation (Steven & Alexandra Cohen Foundation)
Howard Hughes Medical Institute (HHMI)

E-resources Online Full text

NLK Free Medical Journals from 1970 to 6 months ago
Freely Accessible Science Journals from 1995 to 6 months ago
PubMed Central from 1970 to 6 months ago
Europe PubMed Central from 1970 to 6 months ago
Open Access Digital Library from 1970-01-01
Open Access Digital Library from 1970-01-01

Links

PubMed 37846980
DOI 10.1128/iai.00282-23
Knihovny.cz E-resources

Ticks are hematophagous arthropods that use a complex mixture of salivary proteins to evade host defenses while taking a blood meal. Little is known about the immunological and physiological consequences of tick feeding on humans. Here, we performed the first bulk and single-nucleus RNA sequencing (snRNA-seq) of skin and blood of four persons presenting with naturally acquired, attached Ixodes scapularis ticks. Pathways and individual genes associated with innate and adaptive immunity were identified based on bulk RNA sequencing, including interleukin-17 signaling and platelet activation pathways at the site of tick attachment or in peripheral blood. snRNA-seq further revealed that the Hippo signaling, cell adhesion, and axon guidance pathways were involved in the response to an I. scapularis bite in humans. Features of the host response in these individuals also overlapped with that of laboratory guinea pigs exposed to I. scapularis and which acquired resistance to ticks. These findings offer novel insights for the development of new biomarkers for I. scapularis exposure and anti-tick vaccines for human use.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc24000663
003      
CZ-PrNML
005      
20240213093319.0
007      
ta
008      
240109s2023 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1128/iai.00282-23 $2 doi
035    __
$a (PubMed)37846980
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Tang, Xiaotian $u Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine , New Haven, Connecticut, USA $1 https://orcid.org/0000000201719354
245    10
$a Bulk and single-nucleus RNA sequencing highlight immune pathways induced in individuals during an Ixodes scapularis tick bite / $c X. Tang, GE. Lynn, Y. Cui, J. Cerny, G. Arora, MM. Tomayko, J. Craft, E. Fikrig
520    9_
$a Ticks are hematophagous arthropods that use a complex mixture of salivary proteins to evade host defenses while taking a blood meal. Little is known about the immunological and physiological consequences of tick feeding on humans. Here, we performed the first bulk and single-nucleus RNA sequencing (snRNA-seq) of skin and blood of four persons presenting with naturally acquired, attached Ixodes scapularis ticks. Pathways and individual genes associated with innate and adaptive immunity were identified based on bulk RNA sequencing, including interleukin-17 signaling and platelet activation pathways at the site of tick attachment or in peripheral blood. snRNA-seq further revealed that the Hippo signaling, cell adhesion, and axon guidance pathways were involved in the response to an I. scapularis bite in humans. Features of the host response in these individuals also overlapped with that of laboratory guinea pigs exposed to I. scapularis and which acquired resistance to ticks. These findings offer novel insights for the development of new biomarkers for I. scapularis exposure and anti-tick vaccines for human use.
650    _2
$a lidé $7 D006801
650    _2
$a zvířata $7 D000818
650    _2
$a morčata $7 D006168
650    12
$a klíště $x genetika $7 D018884
650    12
$a kousnutí klíštětem $7 D064927
650    _2
$a sekvence nukleotidů $7 D001483
650    _2
$a stravovací zvyklosti $x fyziologie $7 D005247
650    _2
$a RNA malá jaderná $7 D012342
655    _2
$a časopisecké články $7 D016428
700    1_
$a Lynn, Geoffrey E $u Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine , New Haven, Connecticut, USA $1 https://orcid.org/0000000270950912
700    1_
$a Cui, Yingjun $u Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine , New Haven, Connecticut, USA
700    1_
$a Cerny, Jiri $u Czech University of Life Sciences Prague , Praha-Suchdol, Czechia
700    1_
$a Arora, Gunjan $u Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine , New Haven, Connecticut, USA
700    1_
$a Tomayko, Mary M $u Department of Dermatology, Yale University School of Medicine , New Haven, Connecticut, USA
700    1_
$a Craft, Joseph $u Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine , New Haven, Connecticut, USA $u Department of Immunobiology, Yale University School of Medicine , New Haven, Connecticut, USA
700    1_
$a Fikrig, Erol $u Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine , New Haven, Connecticut, USA $1 https://orcid.org/0000000258846047
773    0_
$w MED00002235 $t Infection and immunity $x 1098-5522 $g Roč. 91, č. 11 (2023), s. e0028223
856    41
$u https://pubmed.ncbi.nlm.nih.gov/37846980 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20240109 $b ABA008
991    __
$a 20240213093316 $b ABA008
999    __
$a ok $b bmc $g 2049358 $s 1210357
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2023 $b 91 $c 11 $d e0028223 $e 20231017 $i 1098-5522 $m Infection and immunity $n Infect Immun $x MED00002235
GRA    __
$a 126033 $p HHS | National Institutes of Health (NIH)
GRA    __
$a 138949 $p HHS | National Institutes of Health (NIH)
GRA    __
$a 52206 $p HHS | National Institutes of Health (NIH)
GRA    __
$p Steven and Alexandra Cohen Foundation (Steven & Alexandra Cohen Foundation)
GRA    __
$p Howard Hughes Medical Institute (HHMI)
LZP    __
$a Pubmed-20240109

Find record

Citation metrics

Archiving options