Staphylococcus aureus is one of the most common bacterial pathogens, often asymptomatically colonizing healthy people, but capable of causing fatal disease. The ability to treat S. aureus infections is limited by the rapid spread of multidrug-resistant strains. This study aimed to determine the prevalence of S. aureus carriage among students from Okada, Edo State, Nigeria, to analyze the antibiotic resistance patterns and molecular characteristics of S. aureus isolates. One hundred healthy students from Okada, Nigeria, were tested for nasal colonization by S. aureus. Isolates were identified using standard microbiological methods. The susceptibilities of the isolates to a panel of 22 antimicrobials were tested. spa and staphylococcal cassette chromosome mec typing were performed. The prevalence of S. aureus and methicillin-resistant S. aureus (MRSA) among the students was 23% and 6%, respectively. Of the six (26.1%; 6/23) MRSA isolates detected, CC88-MRSA-IVa (n = 2) and CC7-MRSA-V (n = 2) were the most frequent clones. The CC7-MRSA-V isolates were resistant to multiple antimicrobials. Overall, resistance to beta-lactams, tetracyclines, fluoroquinolones, and aminoglycosides was detected among the S. aureus and MRSA isolates. The high prevalence of MRSA and methicillin-susceptible isolates with resistance to multiple antimicrobial classes observed among the students is an alarming finding. This study indicated the circulation of resistant clones of S. aureus in Nigerian educational institutions and the community.

Department of Biological Sciences College of Natural and Applied Sciences Igbinedion University Okada Okada Edo State Nigeria

Department of Laboratory Medicine Igbinedion University Teaching Hospital Okada Edo State Nigeria

Department of Medical Microbiology Charles University 2nd Faculty of Medicine and Motol University Hospital Prague Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc24000853

003      

CZ-PrNML

005      

20240213093422.0

007      

ta

008      

240109s2023 xxu f 000 0|eng||

009      

AR

024    7_

$a 10.1089/mdr.2023.0001 $2 doi

035    __

$a (PubMed)37713290

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a xxu

100    1_

$a Okwu, Maureen Uchechukwu $u Department of Biological Sciences (Microbiology), College of Natural and Applied Sciences, Igbinedion University Okada, Okada, Edo State, Nigeria

245    10

$a High Frequency of Methicillin-Resistant and Multidrug-Resistant Strains of Staphylococcus aureus Colonizing Students in Okada, Edo State, Nigeria / $c MU. Okwu, AO. Akpoka, O. Mitsan, OE. Izevbuwa, A. Osamede, J. Tkadlec

520    9_

$a Staphylococcus aureus is one of the most common bacterial pathogens, often asymptomatically colonizing healthy people, but capable of causing fatal disease. The ability to treat S. aureus infections is limited by the rapid spread of multidrug-resistant strains. This study aimed to determine the prevalence of S. aureus carriage among students from Okada, Edo State, Nigeria, to analyze the antibiotic resistance patterns and molecular characteristics of S. aureus isolates. One hundred healthy students from Okada, Nigeria, were tested for nasal colonization by S. aureus. Isolates were identified using standard microbiological methods. The susceptibilities of the isolates to a panel of 22 antimicrobials were tested. spa and staphylococcal cassette chromosome mec typing were performed. The prevalence of S. aureus and methicillin-resistant S. aureus (MRSA) among the students was 23% and 6%, respectively. Of the six (26.1%; 6/23) MRSA isolates detected, CC88-MRSA-IVa (n = 2) and CC7-MRSA-V (n = 2) were the most frequent clones. The CC7-MRSA-V isolates were resistant to multiple antimicrobials. Overall, resistance to beta-lactams, tetracyclines, fluoroquinolones, and aminoglycosides was detected among the S. aureus and MRSA isolates. The high prevalence of MRSA and methicillin-susceptible isolates with resistance to multiple antimicrobial classes observed among the students is an alarming finding. This study indicated the circulation of resistant clones of S. aureus in Nigerian educational institutions and the community.

650    _2

$a lidé $7 D006801

650    _2

$a Staphylococcus aureus $7 D013211

650    _2

$a rezistence na methicilin $7 D016106

650    12

$a methicilin rezistentní Staphylococcus aureus $7 D055624

650    _2

$a antibakteriální látky $x farmakologie $7 D000900

650    _2

$a mikrobiální testy citlivosti $7 D008826

650    12

$a stafylokokové infekce $x farmakoterapie $x epidemiologie $x mikrobiologie $7 D013203

650    _2

$a studenti $7 D013334

651    _2

$a Nigérie $x epidemiologie $7 D009549

655    _2

$a časopisecké články $7 D016428

700    1_

$a Akpoka, Augustine Obhioze $u Department of Biological Sciences (Microbiology), College of Natural and Applied Sciences, Igbinedion University Okada, Okada, Edo State, Nigeria

700    1_

$a Mitsan, Olley $u Department of Laboratory Medicine, Igbinedion University Teaching Hospital, Okada, Edo State, Nigeria

700    1_

$a Izevbuwa, Osazee Ekundayo $u Department of Biological Sciences (Microbiology), College of Natural and Applied Sciences, Igbinedion University Okada, Okada, Edo State, Nigeria

700    1_

$a Osamede, Anita $u Department of Biological Sciences (Microbiology), College of Natural and Applied Sciences, Igbinedion University Okada, Okada, Edo State, Nigeria

700    1_

$a Tkadlec, Jan $u Department of Medical Microbiology, Charles University, 2nd Faculty of Medicine and Motol University Hospital, Prague, Czech Republic

773    0_

$w MED00008749 $t Microbial drug resistance $x 1931-8448 $g Roč. 29, č. 11 (2023), s. 516-522

856    41

$u https://pubmed.ncbi.nlm.nih.gov/37713290 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y - $z 0

990    __

$a 20240109 $b ABA008

991    __

$a 20240213093420 $b ABA008

999    __

$a ok $b bmc $g 2049454 $s 1210547

BAS    __

$a 3

BAS    __

$a PreBMC-MEDLINE

BMC    __

$a 2023 $b 29 $c 11 $d 516-522 $e 20230915 $i 1931-8448 $m Microbial drug resistance $n Microb Drug Resist $x MED00008749

LZP    __

$a Pubmed-20240109