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Protection of the endothelium and endothelial glycocalyx by hydrogen against ischaemia-reperfusion injury in a porcine model of cardiac arrest
D. Astapenko, R. Hyspler, A. Ticha, A. Tomasova, P. Navratil, M. Zrzavecky, B. Byreddy, P. Sedlacek, V. Radochova, R. Skulec, RG. Hahn, C. Lehmann, MLNG. Malbrain, V. Cerny
Language English Country Netherlands
Document type Journal Article
PubMed
37694356
DOI
10.3233/ch-231768
Knihovny.cz E-resources
- MeSH
- Endothelium MeSH
- Glycocalyx MeSH
- Cardiopulmonary Resuscitation * MeSH
- Humans MeSH
- Disease Models, Animal MeSH
- Swine MeSH
- Reperfusion Injury * MeSH
- Heart Arrest * therapy MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
BACKGROUND: Hydrogen is a potent antioxidant agent that can easily be administered by inhalation. The aim of the study was to evaluate whether hydrogen protects the endothelial glycocalyx layer after successful cardiopulmonary resuscitation (CPR). METHODS: Fourteen anesthetized pigs underwent CPR after induced ventricular fibrillation. During CPR and return of spontaneous circulation, 2% hydrogen gas was administered to seven pigs (hydrogen group) and seven constituted a control group. Biochemistry and sublingual microcirculation were assessed at baseline, during CPR, at the 15th, 30th, 60th, 120th minute. RESULTS: All seven subjects from the hydrogen group and six subjects in the control group were successfully resuscitated after 6-10 minutes. At baseline, there were no statistically significant differences in examined variables. After the CPR, blood pH, base excess, and lactate showed significantly smaller deterioration in the hydrogen group than in the control group. By contrast, plasma syndecan-1 and the measured variables obtained via sublingual microcirculation did not change after the CPR; and were virtually identical between the two groups. CONCLUSION: In pigs, hydrogen gas inhalation during CPR and post-resuscitation care was associated with less pronounced metabolic acidosis compared to controls. However, we could not find evidence of injury to the endothelium or glycocalyx in any studied groups.
1st Department of Anaesthesiology and Intensive Therapy Medical University of Lublin Lublin Poland
Department of Emergency Medicine Hospital Bory Bratislava Slovak Republic
Department of Microbiology and Immunology Dalhousie University Halifax NS Canada
Department of Pharmacology Dalhousie University Halifax NS Canada
Department of Physiology and Biophysics Dalhousie University Halifax NS Canada
Department of Urology University Hospital Hradec Kralove Hradec Kralove Czech Republic
Faculty of Health Studies Technical University in Liberec Liberec Czech Republic
Faculty of Medicine in Hradec Kralove Charles University Prague Czech Republic
Faculty of Military Health Sciences University of Defence Brno Czech Republic
Institute of Postgradual Education in Healthcare Prague Czech Republic
International Fluid Academy Lovenjoel Belgium
Karolinska Institutet at Danderyds Hospital Stockholm Sweden
References provided by Crossref.org
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