Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Tetraspanins from the liver fluke Opisthorchis viverrini stimulate cholangiocyte migration and inflammatory cytokine production

A. Ruangsuwast, MJ. Smout, PJ. Brindley, A. Loukas, T. Laha, S. Chaiyadet

. 2023 ; 70 (-) : . [pub] 20230916

Language English Country Czech Republic

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc24002942

The liver fluke Opisthorchis viverrini (Poirier, 1886) (Digenea) secretes extracellular vesicles (EVs) bearing CD63-like tetraspanins on their surface. Fluke EVs are actively internalised by host cholangiocytes in the bile ducts, where they drive pathology and promote neoplasia through induction of cellular proliferation and secretion of inflammatory cytokines. We investigated the effects of tetraspanins of the CD63 superfamily by co-culturing recombinant forms of the large extracellular loop (LEL) of O. viverrini tetraspanin-2 (rLEL-Ov-TSP-2) and tetraspanin-3 (rLEL-Ov-TSP-3) with non-cancerous human bile duct (H69) and cholangiocarcinoma (CCA, M213) cell lines. The results showed that cell lines co-cultured with excretory/secretory products from adult O. viverrini (Ov-ES) underwent significantly increased cell proliferation at 48 hours but not 24 hours compared to untreated control cells (P < 0.05), whereas rLEL-Ov-TSP-3 co-culture resulted in significantly increased cell proliferation at both 24 hours (P < 0.05) and 48 hours (P < 0.01) time points. In like fashion, H69 cholangiocytes co-cultured with both Ov-ES and rLEL-Ov-TSP-3 underwent significantly elevated Il-6 and Il-8 gene expression for at least one of the time points assessed. Finally, both rLEL-Ov-TSP-2 and rLEL-Ov-TSP-3 significantly enhanced migration of both M213 and H69 cell lines. These findings indicated that O. viverrini CD63 family tetraspanins can promote a cancerous microenvironment by enhancing innate immune responses and migration of biliary epithelial cells.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc24002942
003      
CZ-PrNML
005      
20250110092617.0
007      
ta
008      
240215e20230916xr f 000 0|eng||
009      
AR
024    7_
$a 10.14411/fp.2023.017 $2 doi
035    __
$a (PubMed)37752807
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Ruangsuwast, Apisit $u Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
245    10
$a Tetraspanins from the liver fluke Opisthorchis viverrini stimulate cholangiocyte migration and inflammatory cytokine production / $c A. Ruangsuwast, MJ. Smout, PJ. Brindley, A. Loukas, T. Laha, S. Chaiyadet
520    9_
$a The liver fluke Opisthorchis viverrini (Poirier, 1886) (Digenea) secretes extracellular vesicles (EVs) bearing CD63-like tetraspanins on their surface. Fluke EVs are actively internalised by host cholangiocytes in the bile ducts, where they drive pathology and promote neoplasia through induction of cellular proliferation and secretion of inflammatory cytokines. We investigated the effects of tetraspanins of the CD63 superfamily by co-culturing recombinant forms of the large extracellular loop (LEL) of O. viverrini tetraspanin-2 (rLEL-Ov-TSP-2) and tetraspanin-3 (rLEL-Ov-TSP-3) with non-cancerous human bile duct (H69) and cholangiocarcinoma (CCA, M213) cell lines. The results showed that cell lines co-cultured with excretory/secretory products from adult O. viverrini (Ov-ES) underwent significantly increased cell proliferation at 48 hours but not 24 hours compared to untreated control cells (P < 0.05), whereas rLEL-Ov-TSP-3 co-culture resulted in significantly increased cell proliferation at both 24 hours (P < 0.05) and 48 hours (P < 0.01) time points. In like fashion, H69 cholangiocytes co-cultured with both Ov-ES and rLEL-Ov-TSP-3 underwent significantly elevated Il-6 and Il-8 gene expression for at least one of the time points assessed. Finally, both rLEL-Ov-TSP-2 and rLEL-Ov-TSP-3 significantly enhanced migration of both M213 and H69 cell lines. These findings indicated that O. viverrini CD63 family tetraspanins can promote a cancerous microenvironment by enhancing innate immune responses and migration of biliary epithelial cells.
650    _2
$a dospělí $7 D000328
650    _2
$a lidé $7 D006801
650    _2
$a zvířata $7 D000818
650    12
$a Fasciola hepatica $7 D005210
650    12
$a Opisthorchis $7 D009891
650    _2
$a epitelové buňky $7 D004847
650    _2
$a buněčné linie $7 D002460
650    _2
$a cytokiny $7 D016207
655    _2
$a časopisecké články $7 D016428
700    1_
$a Smout, Michael J $u Australian Institute of Tropical Health and Medicine, James Cook University, Cairns, Queensland, Australia $1 https://orcid.org/0000000169370112
700    1_
$a Brindley, Paul J $u Department of Microbiology, Immunology and Tropical Medicine, and Research Center for Neglected Diseases of Poverty, School of Medicine & Health Sciences, George Washington University, Washington, D.C., USA $1 https://orcid.org/0000000317650002
700    1_
$a Loukas, Alex $u Australian Institute of Tropical Health and Medicine, James Cook University, Cairns, Queensland, Australia $1 https://orcid.org/0000000208968441
700    1_
$a Laha, Thewarach $u Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand $1 https://orcid.org/0000000313349976
700    1_
$a Chaiyadet, Sujittra $u Department of Tropical Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand *Address for correspondence: Sujittra Chaiyadet, Department of Tropical Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002 Thailand. E-mail $1 https://orcid.org/0000000285368589
773    0_
$w MED00011006 $t Folia parasitologica $x 1803-6465 $g Roč. 70 (20230916)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/37752807 $y Pubmed
910    __
$a ABA008 $b online $c $y - $z 0
990    __
$a 20240215 $b ABA008
991    __
$a 20250110092610 $b ABA008
999    __
$a kom $b bmc $g 2247138 $s 1212678
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2023 $b 70 $c - $e 20230916 $i 1803-6465 $m Folia parasitologica $n Folia Parasitol (Praha) $x MED00011006
LZP    __
$a Pubmed-20240215

Find record

Citation metrics

Archiving options