-
Something wrong with this record ?
A Pilot Study on the Uptake of Propidium Iodide and YO-PRO-1 Iodide through the Pannexin Channels in Wallachian Frozen-Thawed Ram Spermatozoa
FG. Savvulidi, M. Ptáček, A. Málková, E. Golovina, J. Pytlík, M. Janošíková, S. Nagy, JP. Barbas, L. Stádník, K. Savvulidi Vargová
Language English Country Czech Republic
Document type Journal Article
Grant support
CZ.02.1.01/0.0/0.0/16_019/0000845
European Regional Development Fund
NAZV QK1910156
Ministerstvo Zemědělství
SV21-6-21320
Fakultu Agrobiologie, Potravinových a Prírodních Zdrojů, Česká Zemědělská Univerzita v Praze
SV23-7-21320
Fakultu Agrobiologie, Potravinových a Prírodních Zdrojů, Česká Zemědělská Univerzita v Praze
START/MED/089
lékařská fakulta Univerzity Karlovy
PRIMUS/17/MED/16
Univerzita Karlova v Praze
NLK
Free Medical Journals
from 2000
Freely Accessible Science Journals
from 2000
ProQuest Central
from 2005-01-01
Health & Medicine (ProQuest)
from 2005-01-01
ROAD: Directory of Open Access Scholarly Resources
from 2000
- MeSH
- Coloring Agents MeSH
- Benzoxazoles MeSH
- Quinolinium Compounds * MeSH
- Iodides * MeSH
- Horses MeSH
- Cryopreservation methods veterinary MeSH
- Pilot Projects MeSH
- Propidium MeSH
- Dogs MeSH
- Semen MeSH
- Spermatozoa MeSH
- Semen Preservation * veterinary methods MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Dogs MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Propidium iodide (PI) and YO-PRO-1 (YPI) dyes are routinely used to determine sperm viability in many livestock species. It is commonly accepted that these dyes penetrate only sperm cells with damaged plasma membranes. Recently, however, the mechanism of dye uptake unrelated to damaged plasma membranes, but instead related to pannexin channels in dog and stallion sperm cells was demonstrated. This pilot study aimed to evaluate the role of pannexins in the uptake of PI and YPI dyes on Wallachian frozen-thawed ram spermatozoa by flow cytometry using probenecid, a specific inhibitor of pannexin channels. Additionally, the expression of pannexins in Wallachian sperm was evaluated directly (by qRT-PCR). The results demonstrate the active role of pannexin channels in the uptake of PI and YPI dyes on frozen-thawed Wallachian ram sperm. In conclusion, when using the PI or YPI exclusion assay to determine Wallachian frozen-thawed ram sperm viability, the danger of overestimating the number of spermatozoa with the damaged plasma membrane must be considered. The observed breed-specific, and more importantly, individual differences in gene expression as well as in dye uptake indicate the need for further studies.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc24003874
- 003
- CZ-PrNML
- 005
- 20250121100653.0
- 007
- ta
- 008
- 240305s2023 xr d f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.14712/fb2023069040127 $2 doi
- 035 __
- $a (PubMed)38410970
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Savvulidi, Filipp Geirgievich $u Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Czech Republic $7 xx0129339
- 245 12
- $a A Pilot Study on the Uptake of Propidium Iodide and YO-PRO-1 Iodide through the Pannexin Channels in Wallachian Frozen-Thawed Ram Spermatozoa / $c FG. Savvulidi, M. Ptáček, A. Málková, E. Golovina, J. Pytlík, M. Janošíková, S. Nagy, JP. Barbas, L. Stádník, K. Savvulidi Vargová
- 520 9_
- $a Propidium iodide (PI) and YO-PRO-1 (YPI) dyes are routinely used to determine sperm viability in many livestock species. It is commonly accepted that these dyes penetrate only sperm cells with damaged plasma membranes. Recently, however, the mechanism of dye uptake unrelated to damaged plasma membranes, but instead related to pannexin channels in dog and stallion sperm cells was demonstrated. This pilot study aimed to evaluate the role of pannexins in the uptake of PI and YPI dyes on Wallachian frozen-thawed ram spermatozoa by flow cytometry using probenecid, a specific inhibitor of pannexin channels. Additionally, the expression of pannexins in Wallachian sperm was evaluated directly (by qRT-PCR). The results demonstrate the active role of pannexin channels in the uptake of PI and YPI dyes on frozen-thawed Wallachian ram sperm. In conclusion, when using the PI or YPI exclusion assay to determine Wallachian frozen-thawed ram sperm viability, the danger of overestimating the number of spermatozoa with the damaged plasma membrane must be considered. The observed breed-specific, and more importantly, individual differences in gene expression as well as in dye uptake indicate the need for further studies.
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a koně $7 D006736
- 650 _2
- $a psi $7 D004285
- 650 _2
- $a propidium $7 D011419
- 650 _2
- $a pilotní projekty $7 D010865
- 650 12
- $a jodidy $7 D007454
- 650 _2
- $a sperma $7 D012661
- 650 _2
- $a kryoprezervace $x metody $x veterinární $7 D015925
- 650 12
- $a uchování spermatu $x veterinární $x metody $7 D012662
- 650 _2
- $a spermie $7 D013094
- 650 _2
- $a barvicí látky $7 D004396
- 650 _2
- $a benzoxazoly $7 D001583
- 650 12
- $a chinolinové sloučeniny $7 D011806
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Ptáček, Martin $u Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Czech Republic $7 xx0327815
- 700 1_
- $a Málková, Anežka $u Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Czech Republic $7 xx0327814
- 700 1_
- $a Golovina, Elena $u Institute of Pathological Physiology, First Faculty of Medicine, Charles University, Prague, Czech Republic $7 _AN121477
- 700 1_
- $a Pytlík, Jan $u Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Czech Republic $7 xx0327813
- 700 1_
- $a Janošíková, Martina $u Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Czech Republic $7 xx0327812
- 700 1_
- $a Nagy, Szabolcs $u Institute of Animal Sciences, Hungarian University of Agriculture and Life Sciences, Georgikon Campus, Keszthely, Hungary
- 700 1_
- $a Barbas, Joăo Pedro $u Instituto Nacional de Investigaçăo Agrária e Veterinária, Quinta da Fonte Boa, Vale de Santarém, Portugal
- 700 1_
- $a Stádník, Luděk $u Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Czech Republic $7 uzp2006324610
- 700 1_
- $a Savvulidi Vargová, Karina $u Institute of Pathological Physiology, First Faculty of Medicine, Charles University, Prague, Czech Republic. vargova.mmbd@gmail.com $7 xx0172018
- 773 0_
- $w MED00011004 $t Folia biologica $x 0015-5500 $g Roč. 69, č. 4 (2023), s. 127-132
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/38410970 $y Pubmed
- 910 __
- $a ABA008 $b A 970 $c 89 $y p $z 0
- 990 __
- $a 20240305 $b ABA008
- 991 __
- $a 20250121100650 $b ABA008
- 999 __
- $a ok $b bmc $g 2247156 $s 1213626
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2023 $b 69 $c 4 $d 127-132 $e - $i 0015-5500 $m Folia biologica $n Folia Biol (Praha) $x MED00011004
- GRA __
- $a CZ.02.1.01/0.0/0.0/16_019/0000845 $p European Regional Development Fund
- GRA __
- $a NAZV QK1910156 $p Ministerstvo Zemědělství
- GRA __
- $a SV21-6-21320 $p Fakultu Agrobiologie, Potravinových a Prírodních Zdrojů, Česká Zemědělská Univerzita v Praze
- GRA __
- $a SV23-7-21320 $p Fakultu Agrobiologie, Potravinových a Prírodních Zdrojů, Česká Zemědělská Univerzita v Praze
- GRA __
- $a START/MED/089 $p lékařská fakulta Univerzity Karlovy
- GRA __
- $a PRIMUS/17/MED/16 $p Univerzita Karlova v Praze
- LZP __
- $b NLK138 $a Pubmed-20240305
