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Draft genome of neotropical Bacillus thuringiensis UFT038 and its potential against lepidopteran soybean pests
GB. Alves, TSO. Lemes, EJG. Pereira, JL. Jurat-Fuentes, G. Smagghe, GR. Santos, K. Haddi, RFT. Corrêa, FL. Melo, LOV. Jumbo, EE. Oliveira, AJ. Peron, BM. Ribeiro, RWS. Aguiar
Jazyk angličtina Země Česko
Typ dokumentu časopisecké články
- MeSH
- Bacillus thuringiensis * genetika metabolismus MeSH
- bakteriální proteiny genetika metabolismus MeSH
- biologická kontrola škůdců MeSH
- endotoxiny metabolismus MeSH
- faktory virulence metabolismus MeSH
- Glycine max MeSH
- hemolyziny genetika metabolismus farmakologie MeSH
- insekticidy * farmakologie metabolismus MeSH
- larva MeSH
- lidé MeSH
- můry * MeSH
- novorozenec MeSH
- Spodoptera metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- novorozenec MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Bacillus thuringiensis (Bt) is known for its Cry and Vip3A pesticidal proteins with high selectivity to target pests. Here, we assessed the potential of a novel neotropical Bt strain (UFT038) against six lepidopteran pests, including two Cry-resistant populations of fall armyworm, Spodoptera frugiperda. We also sequenced and analyzed the genome of Bt UFT038 to identify genes involved in insecticidal activities or encoding other virulence factors. In toxicological bioassays, Bt UFT038 killed and inhibited the neonate growth in a concentration-dependent manner. Bt UFT038 and HD-1 were equally toxic against S. cosmioides, S. frugiperda (S_Bt and R_Cry1 + 2Ab populations), Helicoverpa zea, and H. armigera. However, larval growth inhibition results indicated that Bt UFT038 was more toxic than HD-1 to S. cosmioides, while HD-1 was more active against Chrysodeixis includens. The draft genome of Bt UFT038 showed the cry1Aa8, cry1Ac11, cry1Ia44, cry2Aa9, cry2Ab35, and vip3Af5 genes. Besides this, genes encoding the virulence factors (inhA, plcA, piplC, sph, and chi1-2) and toxins (alo, cytK, hlyIII, hblA-D, and nheA-C) were also identified. Collectively, our findings reveal the potential of the Bt UFT038 strain as a source of insecticidal genes against lepidopteran pests, including S. cosmioides and S. frugiperda.
Carrera de Agronomía Universidad Nacional de Loja Loja 110103 Ecuador
Departamento de Biologia Celular Universidade de Brasília Brasília DF 70910 900 Brazil
Departamento de Biotecnologia Universidade Federal de Tocantins Gurupi TO 77413 070 Brazil
Departamento de Entomologia Universidade Federal de Lavras Lavras MG 37200 900 Brazil
Departamento de Entomologia Universidade Federal de Viçosa Viçosa MG 36570 900 Brazil
Department of Entomology and Plant Pathology University of Tennessee Knoxville TN 37996 USA
Citace poskytuje Crossref.org
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- $a Bacillus thuringiensis (Bt) is known for its Cry and Vip3A pesticidal proteins with high selectivity to target pests. Here, we assessed the potential of a novel neotropical Bt strain (UFT038) against six lepidopteran pests, including two Cry-resistant populations of fall armyworm, Spodoptera frugiperda. We also sequenced and analyzed the genome of Bt UFT038 to identify genes involved in insecticidal activities or encoding other virulence factors. In toxicological bioassays, Bt UFT038 killed and inhibited the neonate growth in a concentration-dependent manner. Bt UFT038 and HD-1 were equally toxic against S. cosmioides, S. frugiperda (S_Bt and R_Cry1 + 2Ab populations), Helicoverpa zea, and H. armigera. However, larval growth inhibition results indicated that Bt UFT038 was more toxic than HD-1 to S. cosmioides, while HD-1 was more active against Chrysodeixis includens. The draft genome of Bt UFT038 showed the cry1Aa8, cry1Ac11, cry1Ia44, cry2Aa9, cry2Ab35, and vip3Af5 genes. Besides this, genes encoding the virulence factors (inhA, plcA, piplC, sph, and chi1-2) and toxins (alo, cytK, hlyIII, hblA-D, and nheA-C) were also identified. Collectively, our findings reveal the potential of the Bt UFT038 strain as a source of insecticidal genes against lepidopteran pests, including S. cosmioides and S. frugiperda.
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