• Je něco špatně v tomto záznamu ?

Peripheral administration of lipidized NPAF and NPFF analogs does not influence central food intake regulation but induces anxiety-like behavior

V. Strnadová, A. Morgan, M. Škrlová, E. Haasová, K. Bardová, A. Myšková, D. Sýkora, J. Kuneš, B. Železná, L. Maletínská

. 2024 ; 104 (-) : 102417. [pub] 20240223

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc24006535

RF-amide peptides influence multiple physiological processes, including the regulation of appetite, stress responses, behavior, and reproductive and endocrine functions. In this study, we examined the roles of neuropeptide FF receptors (NPFFR1 and NPFFR2) by generating several lipidized analogs of neuropeptide AF (NPAF) and 1DMe, a stable analog of neuropeptide FF (NPFF). These analogs were administered peripherally for the first time to investigate their effects on food intake and other potential physiological outcomes. Lipidized NPAF and 1DMe analogs exhibited enhanced stability and increased pharmacokinetics. These analogs demonstrated preserved high affinity for NPFFR2 in the nanomolar range, while the binding affinity for NPFFR1 was tens of nanomoles. They activated the ERK and Akt signaling pathways in cells overexpressing the NPFFR1 and NPFFR2 receptors. Acute food intake in fasted mice decreased after the peripheral administration of oct-NPAF or oct-1DMe. However, this effect was not as pronounced as that observed after the injection of palm11-PrRP31, a potent anorexigenic compound used as a comparator that binds to GPR10 and the NPFFR2 receptor with high affinity. Neither oct-1DMe nor oct-NPAF decreased food intake or body weight in mice with diet-induced obesity during long-term treatment. In mice treated with oct-1DMe, we observed decreased activity in the central zone during the open field test and decreased activity in the open arms of the elevated plus maze. Furthermore, we observed a decrease in plasma noradrenaline levels and an increase in plasma corticosterone levels, as well as an increase in Crh expression in the hypothalamus. Moreover, neuronal activity in the hypothalamus was increased after treatment with oct-1DMe. In this study, we report that oct-1DMe did not have any long-term effects on the central regulation of food intake; however, it caused anxiety-like behavior.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc24006535
003      
CZ-PrNML
005      
20240423155334.0
007      
ta
008      
240412e20240223ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.npep.2024.102417 $2 doi
035    __
$a (PubMed)38422597
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Strnadová, Veronika $u Institute of Organic Chemistry and Biochemistry, CAS, Prague, Czech Republic
245    10
$a Peripheral administration of lipidized NPAF and NPFF analogs does not influence central food intake regulation but induces anxiety-like behavior / $c V. Strnadová, A. Morgan, M. Škrlová, E. Haasová, K. Bardová, A. Myšková, D. Sýkora, J. Kuneš, B. Železná, L. Maletínská
520    9_
$a RF-amide peptides influence multiple physiological processes, including the regulation of appetite, stress responses, behavior, and reproductive and endocrine functions. In this study, we examined the roles of neuropeptide FF receptors (NPFFR1 and NPFFR2) by generating several lipidized analogs of neuropeptide AF (NPAF) and 1DMe, a stable analog of neuropeptide FF (NPFF). These analogs were administered peripherally for the first time to investigate their effects on food intake and other potential physiological outcomes. Lipidized NPAF and 1DMe analogs exhibited enhanced stability and increased pharmacokinetics. These analogs demonstrated preserved high affinity for NPFFR2 in the nanomolar range, while the binding affinity for NPFFR1 was tens of nanomoles. They activated the ERK and Akt signaling pathways in cells overexpressing the NPFFR1 and NPFFR2 receptors. Acute food intake in fasted mice decreased after the peripheral administration of oct-NPAF or oct-1DMe. However, this effect was not as pronounced as that observed after the injection of palm11-PrRP31, a potent anorexigenic compound used as a comparator that binds to GPR10 and the NPFFR2 receptor with high affinity. Neither oct-1DMe nor oct-NPAF decreased food intake or body weight in mice with diet-induced obesity during long-term treatment. In mice treated with oct-1DMe, we observed decreased activity in the central zone during the open field test and decreased activity in the open arms of the elevated plus maze. Furthermore, we observed a decrease in plasma noradrenaline levels and an increase in plasma corticosterone levels, as well as an increase in Crh expression in the hypothalamus. Moreover, neuronal activity in the hypothalamus was increased after treatment with oct-1DMe. In this study, we report that oct-1DMe did not have any long-term effects on the central regulation of food intake; however, it caused anxiety-like behavior.
650    _2
$a myši $7 D051379
650    _2
$a zvířata $7 D000818
650    12
$a regulace chuti k jídlu $7 D001069
650    12
$a oligopeptidy $x farmakologie $7 D009842
650    _2
$a receptory neuropeptidů $x metabolismus $7 D018013
650    _2
$a úzkost $7 D001007
655    _2
$a časopisecké články $7 D016428
700    1_
$a Morgan, Alena $u Institute of Organic Chemistry and Biochemistry, CAS, Prague, Czech Republic
700    1_
$a Škrlová, Magdalena $u Institute of Organic Chemistry and Biochemistry, CAS, Prague, Czech Republic; First Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Haasová, Eliška $u Institute of Physiology, CAS, Prague, Czech Republic; Department of Physiology, Faculty of Science, Charles University, Prague, Czech Republic
700    1_
$a Bardová, Kristina $u Institute of Physiology, CAS, Prague, Czech Republic
700    1_
$a Myšková, Aneta $u Institute of Organic Chemistry and Biochemistry, CAS, Prague, Czech Republic; Department of Analytical Chemistry, University of Chemistry and Technology Prague, Prague, Czech Republic
700    1_
$a Sýkora, David $u Department of Analytical Chemistry, University of Chemistry and Technology Prague, Prague, Czech Republic
700    1_
$a Kuneš, Jaroslav $u Institute of Organic Chemistry and Biochemistry, CAS, Prague, Czech Republic; Institute of Physiology, CAS, Prague, Czech Republic
700    1_
$a Železná, Blanka $u Institute of Organic Chemistry and Biochemistry, CAS, Prague, Czech Republic
700    1_
$a Maletínská, Lenka $u Institute of Organic Chemistry and Biochemistry, CAS, Prague, Czech Republic. Electronic address: maletin@uochb.cas.cz
773    0_
$w MED00005283 $t Neuropeptides $x 1532-2785 $g Roč. 104 (20240223), s. 102417
856    41
$u https://pubmed.ncbi.nlm.nih.gov/38422597 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20240412 $b ABA008
991    __
$a 20240423155330 $b ABA008
999    __
$a ok $b bmc $g 2080868 $s 1216302
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2024 $b 104 $c - $d 102417 $e 20240223 $i 1532-2785 $m Neuropeptides $n Neuropeptides $x MED00005283
LZP    __
$a Pubmed-20240412

Najít záznam

Citační ukazatele

Možnosti archivace