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Reduction of Microvessel Number and Length in the Cerebellum of Purkinje Cell Degeneration Mice
Y. Kolinko, M. Kralickova, J. Cendelin
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
Grantová podpora
Cooperatio MED/DIAG
Univerzita Karlova v Praze
CZ.02.1.01/0.0/0.0/16_019/0000787 (FIND)
Ministerstvo Školství, Mládeže a Tělovýchovy
NLK
ProQuest Central
od 2002-03-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2002-01-01 do Před 1 rokem
Nursing & Allied Health Database (ProQuest)
od 2002-03-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2002-03-01 do Před 1 rokem
Psychology Database (ProQuest)
od 2002-03-01 do Před 1 rokem
- MeSH
- mikrocévy MeSH
- mozeček * MeSH
- myši - mutanty neurologické MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- Purkyňovy buňky * fyziologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Degenerative effects of nerve tissues are often accompanied by changes in vascularization. In this regard, knowledge about hereditary cerebellar degeneration is limited. In this study, we compared the vascularity of the individual cerebellar components of 3-month-old wild-type mice (n = 8) and Purkinje cell degeneration (pcd) mutant mice, which represent a model of hereditary cerebellar degeneration (n = 8). Systematic random samples of tissue sections were processed, and laminin was immunostained to visualize microvessels. A computer-assisted stereology system was used to quantify microvessel parameters including total number, total length, and associated densities in cerebellar layers. Our results in pcd mice revealed a 45% (p < 0.01) reduction in the total volume of the cerebellum, a 28% (p < 0.05) reduction in the total number of vessels and a lower total length, approaching 50% (p < 0.001), compared to the control mice. In pcd mutants, cerebellar degeneration is accompanied by significant reduction in the microvascular network that is proportional to the cerebellar volume reduction therefore does not change density of in the cerebellar gray matter of pcd mice.
Biomedical Center in Pilsen Faculty of Medicine in Pilsen Charles University Pilsen Czech Republic
Department of Pathophysiology Faculty of Medicine in Pilsen Charles University Pilsen Czech Republic
Citace poskytuje Crossref.org
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