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A Fast HPLC/UV Method for Determination of Ketoprofen in Cellular Media

O. Vozniuk, Z. Kejík, K. Veselá, M. Skaličková, P. Novotný, R. Hromádka, J. Hajduch, P. Martásek, M. Jakubek

. 2024 ; 13 (3) : e202300147. [pub] 20231113

Jazyk angličtina Země Německo

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc24006984

Grantová podpora
SVV260521 Charles University in Prague
UNCE 204064 Charles University in Prague
Progress Q26-38/LF1 Charles University in Prague
Prague Progress Q27/LF1 Charles University in Prague
LM2023053 (EATRIS-CZ) Ministry of Education, Youth, and Sports of Czech Republic
Cooperation Program of Charles University
TN02000109 Technology Agency of the Czech Republic
NU21-08-00407 Ministry of Health of Czech Republic
NU22-D-136 Ministry of Health of Czech Republic
NU22-08-00160 Ministry of Health of Czech Republic
LX22NPO5107 National Institute for Neurological Research
European Union - Next Generation EU

A simple, sensitive and quick HPLC method was developed for the determination of ketoprofen in cell culture media (EMEM, DMEM, RPMI). Separation was performed using a gradient on the C18 column with a mobile phase of acetonitrile and miliQ water acidified by 0.1 % (v/v) formic acid. The method was validated for parameters including linearity, accuracy, precision, limit of quantitation and limit of detection, as well as robustness. The response was found linear over the range of 3-100 μg/mL as demonstrated by the acquired value of correlation coefficient R2=0.9997. The described method is applicable for determination of various pharmacokinetic aspects of ketoprofen in vitro.

Citace poskytuje Crossref.org

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