• Je něco špatně v tomto záznamu ?

Recombinase-aided amplification coupled with lateral flow dipstick for efficient and accurate detection of Bombyx mori nucleopolyhedrovirus

R. Wang, S. Xu, E. Wei, P. He, Y. Zhang, Q. Wang, X. Tang, Z. Shen

. 2024 ; 69 (3) : 667-676. [pub] 20231112

Status minimální Jazyk angličtina Země Česko

Typ dokumentu časopisecké články, hodnotící studie

Perzistentní odkaz   https://www.medvik.cz/link/bmc24009812

The infection of Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the main causes of economic losses in sericulture. Thus, it is essential to establish rapid and effective method for BmNPV detection. In the present study, we have developed a recombinase-aided amplification (RAA) to amplify the BmNPV genomic DNA at 37 °C within 30 min, and achieved a rapid detection method by coupling with a lateral flow dipstick (LFD). The RAA-LFD method had a satisfactory detection limit of 6 copies/μL of recombinant plasmid pMD19-T-IE1, and BmNPV infection of silkworm can be detected 12 h post-infection. This method was highly specific for BmNPV, and without cross-reactivity to other silkworm pathogens. In contrast to conventional polymerase chain reaction (PCR), the RAA-LFD assay showed higher sensitivity, cost-saving, and especially is apt to on-site detection of BmNPV infection in the sericulture production.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc24009812
003      
CZ-PrNML
005      
20240606085138.0
007      
ta
008      
240606s2024 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1007/s12223-023-01102-7 $2 doi
035    __
$a (PubMed)37952188
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Wang, Runpeng $u School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China
245    10
$a Recombinase-aided amplification coupled with lateral flow dipstick for efficient and accurate detection of Bombyx mori nucleopolyhedrovirus / $c R. Wang, S. Xu, E. Wei, P. He, Y. Zhang, Q. Wang, X. Tang, Z. Shen
520    9_
$a The infection of Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the main causes of economic losses in sericulture. Thus, it is essential to establish rapid and effective method for BmNPV detection. In the present study, we have developed a recombinase-aided amplification (RAA) to amplify the BmNPV genomic DNA at 37 °C within 30 min, and achieved a rapid detection method by coupling with a lateral flow dipstick (LFD). The RAA-LFD method had a satisfactory detection limit of 6 copies/μL of recombinant plasmid pMD19-T-IE1, and BmNPV infection of silkworm can be detected 12 h post-infection. This method was highly specific for BmNPV, and without cross-reactivity to other silkworm pathogens. In contrast to conventional polymerase chain reaction (PCR), the RAA-LFD assay showed higher sensitivity, cost-saving, and especially is apt to on-site detection of BmNPV infection in the sericulture production.
650    12
$a nukleopolyhedroviry $x genetika $x izolace a purifikace $7 D017924
650    _2
$a zvířata $7 D000818
650    12
$a bourec $x virologie $7 D012831
650    12
$a techniky amplifikace nukleových kyselin $x metody $7 D021141
650    12
$a rekombinasy $x metabolismus $x genetika $7 D045522
650    _2
$a senzitivita a specificita $7 D012680
650    _2
$a DNA virů $x genetika $7 D004279
650    _2
$a limita detekce $7 D057230
655    _2
$a časopisecké články $7 D016428
655    _2
$a hodnotící studie $7 D023362
700    1_
$a Xu, Sheng $u School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China
700    1_
$a Wei, Erjun $u School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China
700    1_
$a He, Ping $u School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China
700    1_
$a Zhang, Yiling $u School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China $u Institute of Sericulture, Chinese Academy of Agricultural Sciences, Zhenjiang, China
700    1_
$a Wang, Qiang $u School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China $u Institute of Sericulture, Chinese Academy of Agricultural Sciences, Zhenjiang, China
700    1_
$a Tang, Xudong $u School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China $u Institute of Sericulture, Chinese Academy of Agricultural Sciences, Zhenjiang, China
700    1_
$a Shen, Zhongyuan $u School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China. szysri@163.com $u Institute of Sericulture, Chinese Academy of Agricultural Sciences, Zhenjiang, China. szysri@163.com $1 https://orcid.org/0000000319779045
773    0_
$w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 69, č. 3 (2024), s. 667-676
856    41
$u https://pubmed.ncbi.nlm.nih.gov/37952188 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20240606 $b ABA008
991    __
$a 20240606085132 $b ABA008
999    __
$a min $b bmc $g 2102563 $s 1219642
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2024 $b 69 $c 3 $d 667-676 $e 20231112 $i 1874-9356 $m Folia microbiologica $n Folia microbiol. (Prague) $x MED00011005
LZP    __
$a Pubmed-20240606

Najít záznam

Citační ukazatele

Možnosti archivace