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Role of the 3-mercaptopyruvate sulfurtransferase in colon/colorectal cancers
K. Matyasova, A. Soltysova, P. Babula, O. Krizanova, V. Liskova
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články
- MeSH
- apoptóza MeSH
- dynaminy metabolismus genetika MeSH
- HCT116 buňky MeSH
- kolorektální nádory * metabolismus patologie enzymologie genetika MeSH
- lidé MeSH
- membránový potenciál mitochondrií MeSH
- mitochondrie metabolismus MeSH
- nádorové buněčné linie MeSH
- nádory tračníku metabolismus patologie genetika enzymologie MeSH
- pohyb buněk MeSH
- proliferace buněk MeSH
- sulfurtransferasy * metabolismus genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
The 3-mercaptopyruvate sulfurtransferase (MPST) is a protein persulfidase, occurring mainly in mitochondria. Although function of this protein in cancer cells has been already studied, no clear outcome can be postulated up to now. Therefore, we focused on the determination of function of MPST in colon (HCT116 cells)/colorectal (DLD1 cells) cancers. In silico analysis revealed that in gastrointestinal cancers, MPST together with its binding partners can be either of a high risk or might have a protective effect. Silencing of MPST gene resulted in decreased ATP, while acetyl-CoA levels were elevated. Increased apoptosis was detected in cells with silenced MPST gene, which was accompanied by decrease in mitochondrial membrane potential, but no changes in IP3 receptor's protein. Mitochondria underwent activation of fission and elevated DRP1 expression after MPST silencing. Proliferation and migration of DLD1 and HCT116 cells were markedly affected, showing the importance of MPST protein in colon/colorectal cancer development.
Department of Molecular Biology Faculty of Natural Sciences Comenius University Bratislava Slovakia
Department of Physiology Faculty of Medicine Masaryk University Brno Czech Republic
Citace poskytuje Crossref.org
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