• Je něco špatně v tomto záznamu ?

Skeletal muscle myosin heavy chain expression and 3D capillary network changes in streptozotocin-induced diabetic female mice

N. Umek, L. Pušnik, CK. Ugwoke, Ž. Šink, S. Horvat, J. Janáček, E. Cvetko

. 2024 ; 24 (3) : 582-592. [pub] 20240502

Jazyk angličtina Země Bosna a Hercegovina

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc24014016

It is not well-understood how type 1 diabetes (T1DM) affects skeletal muscle histological phenotype, particularly capillarisation. This study aimed to analyze skeletal muscle myosin heavy chain (MyHC) fibre type changes and 3D capillary network characteristics in experimental T1DM mice. Female C57BL/6J-OlaHsd mice were categorized into streptozotocin (STZ)-induced diabetic (n = 12) and age-matched non-diabetic controls (n =12). The muscle fibre phenotype of the soleus, gluteus maximus, and gastrocnemius muscles were characterized based on the expression of MyHC isoforms, while capillaries of the gluteus maximus were assessed with immunofluorescence staining, confocal laser microscopy and 3D image analysis. STZ-induced diabetic mice exhibited elevated glucose levels, reduced body weight, and prolonged thermal latency, verifying the T1DM phenotype. In both T1DM and non-diabetic mice, the gluteus maximus and gastrocnemius muscles predominantly expressed fast-twitch type 2b fibers, with no significant differences noted. However, the soleus muscle in non-diabetic mice had a greater proportion of type 2a fibers and comparable type 1 fiber densities (26.2 ± 14.6% vs 21.9 ± 13.5%) relative to diabetic mice. T1DM mice showed reduced fiber diameters (P = 0.026), and the 3D capillary network analysis indicated a higher capillary length per muscle volume in the gluteus maximus of diabetic mice compared to controls (P < 0.05). Overall, T1DM induced significant changes in the skeletal muscle, including shifts in MyHC fibre types, decreased fibre diameters, and increased relative capillarisation, possibly due to muscle fibre atrophy. Our findings emphasize the superior detail provided by the 3D analytical method for characterizing skeletal muscle capillary architecture, highlighting caution in interpreting 2D data for capillary changes in T1DM.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc24014016
003      
CZ-PrNML
005      
20240905134414.0
007      
ta
008      
240725s2024 bn f 000 0|eng||
009      
AR
024    7_
$a 10.17305/bb.2023.9843 $2 doi
035    __
$a (PubMed)37902457
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a bn
100    1_
$a Umek, Nejc $u Institute of Anatomy, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
245    10
$a Skeletal muscle myosin heavy chain expression and 3D capillary network changes in streptozotocin-induced diabetic female mice / $c N. Umek, L. Pušnik, CK. Ugwoke, Ž. Šink, S. Horvat, J. Janáček, E. Cvetko
520    9_
$a It is not well-understood how type 1 diabetes (T1DM) affects skeletal muscle histological phenotype, particularly capillarisation. This study aimed to analyze skeletal muscle myosin heavy chain (MyHC) fibre type changes and 3D capillary network characteristics in experimental T1DM mice. Female C57BL/6J-OlaHsd mice were categorized into streptozotocin (STZ)-induced diabetic (n = 12) and age-matched non-diabetic controls (n =12). The muscle fibre phenotype of the soleus, gluteus maximus, and gastrocnemius muscles were characterized based on the expression of MyHC isoforms, while capillaries of the gluteus maximus were assessed with immunofluorescence staining, confocal laser microscopy and 3D image analysis. STZ-induced diabetic mice exhibited elevated glucose levels, reduced body weight, and prolonged thermal latency, verifying the T1DM phenotype. In both T1DM and non-diabetic mice, the gluteus maximus and gastrocnemius muscles predominantly expressed fast-twitch type 2b fibers, with no significant differences noted. However, the soleus muscle in non-diabetic mice had a greater proportion of type 2a fibers and comparable type 1 fiber densities (26.2 ± 14.6% vs 21.9 ± 13.5%) relative to diabetic mice. T1DM mice showed reduced fiber diameters (P = 0.026), and the 3D capillary network analysis indicated a higher capillary length per muscle volume in the gluteus maximus of diabetic mice compared to controls (P < 0.05). Overall, T1DM induced significant changes in the skeletal muscle, including shifts in MyHC fibre types, decreased fibre diameters, and increased relative capillarisation, possibly due to muscle fibre atrophy. Our findings emphasize the superior detail provided by the 3D analytical method for characterizing skeletal muscle capillary architecture, highlighting caution in interpreting 2D data for capillary changes in T1DM.
650    _2
$a zvířata $7 D000818
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a myši $7 D051379
650    12
$a kapiláry $x patologie $x metabolismus $7 D002196
650    12
$a experimentální diabetes mellitus $x metabolismus $x patologie $7 D003921
650    _2
$a diabetes mellitus 1. typu $x metabolismus $x patologie $7 D003922
650    _2
$a zobrazování trojrozměrné $7 D021621
650    _2
$a myši inbrední C57BL $7 D008810
650    12
$a kosterní svaly $x metabolismus $x patologie $x krevní zásobení $7 D018482
650    12
$a těžké řetězce myosinu $x metabolismus $7 D018995
650    _2
$a streptozocin $7 D013311
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Pušnik, Luka $u Institute of Anatomy, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
700    1_
$a Ugwoke, Chiedozie Kenneth $u Institute of Anatomy, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
700    1_
$a Šink, Žiga $u Institute of Anatomy, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
700    1_
$a Horvat, Simon $u Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
700    1_
$a Janáček, Jiří $u Laboratory of Biomathematics, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic
700    1_
$a Cvetko, Erika $u Institute of Anatomy, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
773    0_
$w MED00210815 $t Biomolecules and biomedicine $x 2831-090X $g Roč. 24, č. 3 (2024), s. 582-592
856    41
$u https://pubmed.ncbi.nlm.nih.gov/37902457 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20240725 $b ABA008
991    __
$a 20240905134408 $b ABA008
999    __
$a ok $b bmc $g 2143670 $s 1225882
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2024 $b 24 $c 3 $d 582-592 $e 20240502 $i 2831-090X $m Biomolecules and biomedicine $n Biomol Biomed $x MED00210815
LZP    __
$a Pubmed-20240725

Najít záznam

Citační ukazatele

Možnosti archivace