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Assessment of twelve echovirus virus-neutralisation assays in Europe: recommendations for harmonisation of non-polio enterovirus sero-surveillance studies
K. Couderé, KSM. Benschop, G. Koen, H. van Eijk, H. Harvala, JL. Bailly, M. Bessaud, E. Kamau, I. Simon, ML. Joffret, L. Nikolaeva-Glomb, I. Georgieva, A. Stoyanova, S. Diedrich, S. Böttcher, M. Cabrerizo, I. Tabain, Ž. Hruškar, V. Stevanović, P....
Language English Country England, Great Britain
Document type Journal Article
NLK
Free Medical Journals
from 1967 to 1 year ago
Freely Accessible Science Journals
from 1967 to 12 months ago
PubMed
39311842
DOI
10.1099/jgv.0.001986
Knihovny.cz E-resources
- MeSH
- Echovirus Infections virology epidemiology immunology MeSH
- Enterovirus Infections virology immunology MeSH
- Enterovirus B, Human * immunology MeSH
- Humans MeSH
- Neutralization Tests * methods standards MeSH
- Antibodies, Neutralizing * blood immunology MeSH
- Antibodies, Viral * blood immunology MeSH
- Seroepidemiologic Studies MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Europe MeSH
Non-polio enteroviruses (NPEV) cause significant disease worldwide. Population-based sero-surveillance, by measuring antibodies against specific NPEV types, provides additional information on past circulation and the prediction for future upsurges. Virus neutralisation assays (VNA), the current method of choice for measuring NPEV type specific antibodies, are not entirely standardised. Via the European Non-Polio Enterovirus Network, we organised a VNA quality assessment in which twelve laboratories participated. We provided five echovirus (E) types (E1, E18, E30 G2, E30 G6 and E6) and intravenous immunoglobulins (IVIG) as a sample for the NPEV VNA quality assessment. Differences in VNA protocols and neutralising Ab (nAb) titres were found between the participating laboratories with geometric coefficients of variation ranging from 10.3-62.9 %. Mixed-effects regression analysis indicated a small but significant effect of type of cell line used. Harmonisation of cell line passage number, however, did not improve variation between laboratories. Calibration by making use of a reference sample, reduced variation between laboratories but differences in nAb titres remained higher than two log2 dilution steps. In conclusion, sero-surveillance data from different laboratories should be compared with caution and standardised protocols are needed.
Cantacuzino National Institute for Medical Military Research and Development Bucharest Romania
Croatian Institute of Public Health Zagreb Croatia
Department of Medical Microbiology OrganoVIR Labs Amsterdam UMC AMC Amsterdam Netherlands
Department of Virology National Center for Infectious and Parasitic Diseases Sofia Bulgaria
Faculty of Veterinary Medicine Zagreb Croatia
https escv eu european non polio enterovirus network enpen
Institute of Biomedicine Faculty of Medicine University of Turku Turku Finland
Microbiology Services NHS Blood and Transplant Colindale UK
National Institute for Public Health and the Environment RIVM Bilthoven Netherlands
National Institute Public Health NRL Enteroviruses Praha Czech Republic
Nuffield Department of Medicine University of Oxford Oxford UK
Radcliffe Department of Medicine University of Oxford Oxford UK
Robert Koch Institute Berlin Germany
School of Molecular and Cellular Biology Faculty of Biological Sciences University of Leeds Leeds UK
References provided by Crossref.org
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