• Something wrong with this record ?

Portable and affordable cold air plasma source with optimized bactericidal effect

M. Klenivskyi, J. Khun, L. Thonová, E. Vaňková, V. Scholtz

. 2024 ; 14 (1) : 15930. [pub] 20240710

Language English Country England, Great Britain

Document type Journal Article

Grant support
21-39019L Grantová Agentura České Republiky

The paper reports a low-cost handheld source of a cold air plasma intended for biomedical applications that can be made by anyone (detailed technical information and a step-by-step guide for creating the NTP source are provided). The plasma source employs a 1.4 W corona discharge in the needle-to-cone electrode configuration and is an extremely simple device, consisting basically of two electrodes and a cheap power supply. To achieve the best bactericidal effect, the plasma source has been optimized on Escherichia coli. The bactericidal ability of the plasma source was further tested on a wide range of microorganisms: Staphylococcus aureus as a representative of gram-positive bacteria, Pseudomonas aeruginosa as gram-negative bacteria, Candida albicans as yeasts, Trichophyton interdigitale as microfungi, and Deinococcus radiodurans as a representative of extremophilic bacteria resistant to many DNA-damaging agents, including ultraviolet and ionizing radiation. The testing showed that the plasma source inactivates all the microorganisms tested in several minutes (up to 105-107 CFU depending on a microorganism), proving its effectiveness against a wide spectrum of pathogens, in particular microfungi, yeasts, gram-positive and gram-negative bacteria. Studies of long-lived reactive species such as ozone, nitrogen oxides, hydrogen peroxide, nitrite, and nitrate revealed a strong correlation between ozone and the bactericidal effect, indicating that the bactericidal effect should generally be attributed to reactive oxygen species. This is the first comprehensive study of the bactericidal effect of a corona discharge in air and the formation of long-lived reactive species by the discharge, depending on both the interelectrode distance and the discharge current.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc24019767
003      
CZ-PrNML
005      
20241024111006.0
007      
ta
008      
241015s2024 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41598-024-66017-w $2 doi
035    __
$a (PubMed)38987305
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Klenivskyi, Myron $u Department of Physics and Measurements, University of Chemistry and Technology, Prague, Czech Republic
245    10
$a Portable and affordable cold air plasma source with optimized bactericidal effect / $c M. Klenivskyi, J. Khun, L. Thonová, E. Vaňková, V. Scholtz
520    9_
$a The paper reports a low-cost handheld source of a cold air plasma intended for biomedical applications that can be made by anyone (detailed technical information and a step-by-step guide for creating the NTP source are provided). The plasma source employs a 1.4 W corona discharge in the needle-to-cone electrode configuration and is an extremely simple device, consisting basically of two electrodes and a cheap power supply. To achieve the best bactericidal effect, the plasma source has been optimized on Escherichia coli. The bactericidal ability of the plasma source was further tested on a wide range of microorganisms: Staphylococcus aureus as a representative of gram-positive bacteria, Pseudomonas aeruginosa as gram-negative bacteria, Candida albicans as yeasts, Trichophyton interdigitale as microfungi, and Deinococcus radiodurans as a representative of extremophilic bacteria resistant to many DNA-damaging agents, including ultraviolet and ionizing radiation. The testing showed that the plasma source inactivates all the microorganisms tested in several minutes (up to 105-107 CFU depending on a microorganism), proving its effectiveness against a wide spectrum of pathogens, in particular microfungi, yeasts, gram-positive and gram-negative bacteria. Studies of long-lived reactive species such as ozone, nitrogen oxides, hydrogen peroxide, nitrite, and nitrate revealed a strong correlation between ozone and the bactericidal effect, indicating that the bactericidal effect should generally be attributed to reactive oxygen species. This is the first comprehensive study of the bactericidal effect of a corona discharge in air and the formation of long-lived reactive species by the discharge, depending on both the interelectrode distance and the discharge current.
650    12
$a plazmové plyny $x farmakologie $7 D058626
650    _2
$a antibakteriální látky $x farmakologie $7 D000900
650    _2
$a Escherichia coli $x účinky léků $7 D004926
650    _2
$a Staphylococcus aureus $x účinky léků $7 D013211
650    _2
$a Candida albicans $x účinky léků $7 D002176
655    _2
$a časopisecké články $7 D016428
700    1_
$a Khun, Josef $u Department of Physics and Measurements, University of Chemistry and Technology, Prague, Czech Republic
700    1_
$a Thonová, Laura $u Department of Physics and Measurements, University of Chemistry and Technology, Prague, Czech Republic $u Department of Physics, Faculty of Nuclear Sciences and Physical Engineering, Czech Technical University in Prague, Prague, Czech Republic
700    1_
$a Vaňková, Eva $u Department of Physics and Measurements, University of Chemistry and Technology, Prague, Czech Republic
700    1_
$a Scholtz, Vladimír $u Department of Physics and Measurements, University of Chemistry and Technology, Prague, Czech Republic. scholtzv@vscht.cz
773    0_
$w MED00182195 $t Scientific reports $x 2045-2322 $g Roč. 14, č. 1 (2024), s. 15930
856    41
$u https://pubmed.ncbi.nlm.nih.gov/38987305 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20241015 $b ABA008
991    __
$a 20241024111000 $b ABA008
999    __
$a ok $b bmc $g 2202161 $s 1231740
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2024 $b 14 $c 1 $d 15930 $e 20240710 $i 2045-2322 $m Scientific reports $n Sci Rep $x MED00182195
GRA    __
$a 21-39019L $p Grantová Agentura České Republiky
LZP    __
$a Pubmed-20241015

Find record

Citation metrics

Loading data ...

Archiving options

Loading data ...