Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Use of the EmsB microsatellite-based next generation sequencing for genotyping of Echinococcus granulosus sensu lato in hydatid cyst tissue samples from animals and humans

S. Ereqat, A. Al-Jawabreh, H. Al-Jawabreh, A. Nasereddin

. 2024 ; 71 (-) : . [pub] 20240805

Jazyk angličtina Země Česko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc25000308

Echinococcus granulosus (Batsch, 1786), a cestode of the Teniidae family, causes human cystic echinococcosis (CE) also known as hydatid disease. Echinococcus granulosus sensu lato includes the G1, G3, G4, G5, G6/7 and G8/10 genotypes which are known to cause human CE. This study aimed to differentiate genotypes of E. granulosus s.l. complex by employing EmsB, a tandemly repeated multilocus microsatellite, using next-generation sequencing (MIC-NGS). Human and animal histopathology-confirmed hydatid cyst tissue samples and reference DNA samples of E. granulosus G1, G3, G4, G5, G6/7 and G10 underwent MIC-NGS assay with custom primers amplifying a 151 bp EmsB DNA fragment. NGS data were analysed using online Galaxy analysis pipeline, a phylogenetic tree was constructed by MEGA software, and haplotype networking was performed with PopArt 1.7. All sixty samples (49 from animals and 11 from humans) included were successfully identified and genotyped with a 100 % success rate. The study showed improved discrimination power to distinguish all study samples including closely related E. granulosus s.s. genotypes G1-G3. The maximum likelihood tree reaffirmed the monophyly of E. granulosus s.l. The median-joining haplotype networking revealed 12 distinct haplotypes. In conclusion, MIC-NGS assay was shown to be sensitive, specific and simple to apply to clinical samples offering a powerful discriminatory tool for the genotyping of E. granulosus s.l.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc25000308
003      
CZ-PrNML
005      
20250110092630.0
007      
ta
008      
250107e20240805xr f 000 0|eng||
009      
AR
024    7_
$a 10.14411/fp.2024.014 $2 doi
035    __
$a (PubMed)39264026
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Ereqat, Suheir $u Biochemistry and Molecular Biology Department, Faculty of Medicine, Al-Quds University, Abu Deis, Jerusalem, Palestine $1 https://orcid.org/0000000337065732
245    10
$a Use of the EmsB microsatellite-based next generation sequencing for genotyping of Echinococcus granulosus sensu lato in hydatid cyst tissue samples from animals and humans / $c S. Ereqat, A. Al-Jawabreh, H. Al-Jawabreh, A. Nasereddin
520    9_
$a Echinococcus granulosus (Batsch, 1786), a cestode of the Teniidae family, causes human cystic echinococcosis (CE) also known as hydatid disease. Echinococcus granulosus sensu lato includes the G1, G3, G4, G5, G6/7 and G8/10 genotypes which are known to cause human CE. This study aimed to differentiate genotypes of E. granulosus s.l. complex by employing EmsB, a tandemly repeated multilocus microsatellite, using next-generation sequencing (MIC-NGS). Human and animal histopathology-confirmed hydatid cyst tissue samples and reference DNA samples of E. granulosus G1, G3, G4, G5, G6/7 and G10 underwent MIC-NGS assay with custom primers amplifying a 151 bp EmsB DNA fragment. NGS data were analysed using online Galaxy analysis pipeline, a phylogenetic tree was constructed by MEGA software, and haplotype networking was performed with PopArt 1.7. All sixty samples (49 from animals and 11 from humans) included were successfully identified and genotyped with a 100 % success rate. The study showed improved discrimination power to distinguish all study samples including closely related E. granulosus s.s. genotypes G1-G3. The maximum likelihood tree reaffirmed the monophyly of E. granulosus s.l. The median-joining haplotype networking revealed 12 distinct haplotypes. In conclusion, MIC-NGS assay was shown to be sensitive, specific and simple to apply to clinical samples offering a powerful discriminatory tool for the genotyping of E. granulosus s.l.
650    _2
$a zvířata $7 D000818
650    12
$a Echinococcus granulosus $x genetika $7 D048209
650    12
$a echinokokóza $x veterinární $x parazitologie $7 D004443
650    12
$a mikrosatelitní repetice $7 D018895
650    12
$a vysoce účinné nukleotidové sekvenování $7 D059014
650    _2
$a lidé $7 D006801
650    12
$a genotyp $7 D005838
650    _2
$a fylogeneze $7 D010802
650    _2
$a genotypizační techniky $x veterinární $7 D060005
655    _2
$a časopisecké články $7 D016428
700    1_
$a Al-Jawabreh, Amer $u Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Arab American University, Jenin, Palestine $u Leishmaniases Research Unit, Jericho, Palestine $1 https://orcid.org/0000000159298743
700    1_
$a Al-Jawabreh, Hanan $u Biochemistry and Molecular Biology Department, Faculty of Medicine, Al-Quds University, Abu Deis, Jerusalem, Palestine $u Leishmaniases Research Unit, Jericho, Palestine $1 https://orcid.org/0000000335615534
700    1_
$a Nasereddin, Abedelmajeed $u Biochemistry and Molecular Biology Department, Faculty of Medicine, Al-Quds University, Abu Deis, Jerusalem, Palestine $u Al-Quds Bard College Al-Quds University Jerusalem, Palestine *Address for correspondence: Al-Quds str., Jericho, Palestine, P5840227. E-mail: islahjr@yahoo.com $1 https://orcid.org/000000018162420X
773    0_
$w MED00011006 $t Folia parasitologica $x 1803-6465 $g Roč. 71 (20240805)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/39264026 $y Pubmed
910    __
$a ABA008 $b online $c $y - $z 0
990    __
$a 20250107 $b ABA008
991    __
$a 20250110092623 $b ABA008
999    __
$a kom $b bmc $g 2247193 $s 1236308
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2024 $b 71 $c - $e 20240805 $i 1803-6465 $m Folia parasitologica $n Folia Parasitol (Praha) $x MED00011006
LZP    __
$a Pubmed-20250107
Zpět

Najít záznam

Citační ukazatele

Možnosti archivace