• Je něco špatně v tomto záznamu ?

Preparation and Complex Characterisation of Stabilised Gold Nanoparticles: Biodistribution and Application for High Resolution In Vivo Imaging

J. Turánek, PT. Knötigová, P. Kulich, R. Skoupý, K. Hrubanová, N. Vaškovicová, L. Fekete, A. Kaňa, R. Mikulík, M. Raška

. 2024 ; 17 (11) : . [pub] 20241103

Status neindexováno Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc25002214

Grantová podpora
NU21-08-00510. The Ministry of Health of the Czech Republic, AZV

The Turkevich method was optimized to prepare gold nanoparticles (AuNP) stabilized by polyethyleneglycol (PEG) for μCT. Using various independent modalities, we thoroughly characterized the optimized PEG-AuNPs. Here, we show that PEG-AuNPs are retained in the blood and provide a high contrast in the high-resolution μCT imaging of blood vessels and inner organs. The biodistribution is characterized by prolonged circulation in the blood and accumulation in the liver, spleen and skin. The accumulation of AuNP in the skin resulted in the blue discoloration of eyes and the whole skin. In vitro experiments using a leukemic monocyte THP-1 cell line model expressing high levels of NLRP3 demonstrated that the NLRP3inflammasome was not activated by PEG AuNP. Over 9 months, the mice were scanned by μCT and were in good health. Scans in mice using PEG-stabilized AuNPs in this study were sharper, with a higher contrast, when compared to a commercial contrasting agent at the same dose. The PEG-AuNPs were morphologically and chemically stable for at least two years when stored in the refrigerator.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc25002214
003      
CZ-PrNML
005      
20250123102018.0
007      
ta
008      
250117s2024 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/ph17111479 $2 doi
035    __
$a (PubMed)39598391
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Turánek, Jaroslav $u Neurology Department, The International Clinical Research Center ICRC of St. Anne's University Hospital in Brno, Pekařská 53, 656 91 Brno, Czech Republic $u Department of Immunology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Hněvotínská 3, 775 15 Olomouc, Czech Republic $u Institute of Clinical Immunology and Allergology, University Hospital Hradec Kralove and Faculty of Medicine in Hradec Kralove, Charles University, Sokolská 581, 50005 Hradec Kralove, Czech Republic
245    10
$a Preparation and Complex Characterisation of Stabilised Gold Nanoparticles: Biodistribution and Application for High Resolution In Vivo Imaging / $c J. Turánek, PT. Knötigová, P. Kulich, R. Skoupý, K. Hrubanová, N. Vaškovicová, L. Fekete, A. Kaňa, R. Mikulík, M. Raška
520    9_
$a The Turkevich method was optimized to prepare gold nanoparticles (AuNP) stabilized by polyethyleneglycol (PEG) for μCT. Using various independent modalities, we thoroughly characterized the optimized PEG-AuNPs. Here, we show that PEG-AuNPs are retained in the blood and provide a high contrast in the high-resolution μCT imaging of blood vessels and inner organs. The biodistribution is characterized by prolonged circulation in the blood and accumulation in the liver, spleen and skin. The accumulation of AuNP in the skin resulted in the blue discoloration of eyes and the whole skin. In vitro experiments using a leukemic monocyte THP-1 cell line model expressing high levels of NLRP3 demonstrated that the NLRP3inflammasome was not activated by PEG AuNP. Over 9 months, the mice were scanned by μCT and were in good health. Scans in mice using PEG-stabilized AuNPs in this study were sharper, with a higher contrast, when compared to a commercial contrasting agent at the same dose. The PEG-AuNPs were morphologically and chemically stable for at least two years when stored in the refrigerator.
590    __
$a NEINDEXOVÁNO
655    _2
$a časopisecké články $7 D016428
700    1_
$a Knötigová, Pavlína Turánek $u Neurology Department, The International Clinical Research Center ICRC of St. Anne's University Hospital in Brno, Pekařská 53, 656 91 Brno, Czech Republic
700    1_
$a Kulich, Pavel $u Veterinary Research Institute, v.v.i., Hudcova 296/70, 621 00 Brno, Czech Republic
700    1_
$a Skoupý, Radim $u Institute of Scientific Instruments, v.v.i., AS CR, Královopolská 147, 612 00 Brno, Czech Republic $1 https://orcid.org/0000000306653873
700    1_
$a Hrubanová, Kamila $u Institute of Scientific Instruments, v.v.i., AS CR, Královopolská 147, 612 00 Brno, Czech Republic
700    1_
$a Vaškovicová, Naděžda $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Kamenice 753/5, 62500 Brno, Czech Republic
700    1_
$a Fekete, Ladislav $u Institute of Physics, Czech Academy of Sciences, Na Slovance 2, 18200 Prague 8, Czech Republic
700    1_
$a Kaňa, Antonín $u Department of Analytical Chemistry, University of Chemistry and Technology, Prague, Technická 5, 166 28 Praha 6, Czech Republic $1 https://orcid.org/0000000338713965 $7 ola20211116748
700    1_
$a Mikulík, Robert $u Neurology Department, The International Clinical Research Center ICRC of St. Anne's University Hospital in Brno, Pekařská 53, 656 91 Brno, Czech Republic
700    1_
$a Raška, Milan $u Department of Immunology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Hněvotínská 3, 775 15 Olomouc, Czech Republic $1 https://orcid.org/0000000285727246 $7 xx0060585
773    0_
$w MED00184066 $t Pharmaceuticals $x 1424-8247 $g Roč. 17, č. 11 (2024)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/39598391 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20250117 $b ABA008
991    __
$a 20250123102012 $b ABA008
999    __
$a ok $b bmc $g 2254503 $s 1238217
BAS    __
$a 3
BAS    __
$a PreBMC-PubMed-not-MEDLINE
BMC    __
$a 2024 $b 17 $c 11 $e 20241103 $i 1424-8247 $m Pharmaceuticals $n Pharmaceuticals (Basel) $x MED00184066
GRA    __
$a NU21-08-00510. $p The Ministry of Health of the Czech Republic, AZV
LZP    __
$a Pubmed-20250117

Najít záznam

Citační ukazatele

Možnosti archivace