This study examined the sequences of the two rRNA (rrn) operons of pathogenic non-cultivable treponemes, comprising 11 strains of T. pallidum ssp. pallidum (TPA), five strains of T. pallidum ssp. pertenue (TPE), two strains of T. pallidum ssp. endemicum (TEN), a simian Fribourg-Blanc strain and a rabbit T. paraluiscuniculi (TPc) strain. PCR was used to determine the type of 16S-23S ribosomal intergenic spacers in the rrn operons from 30 clinical samples belonging to five different genotypes. When compared with the TPA strains, TPc Cuniculi A strain had a 17 bp deletion, and the TPE, TEN and Fribourg-Blanc isolates had a deletion of 33 bp. Other than these deletions, only 17 heterogeneous sites were found within the entire region (excluding the 16S-23S intergenic spacer region encoding tRNA-Ile or tRNA-Ala). The pattern of nucleotide changes in the rrn operons corresponded to the classification of treponemal strains, whilst two different rrn spacer patterns (Ile/Ala and Ala/Ile) appeared to be distributed randomly across species/subspecies classification, time and geographical source of the treponemal strains. It is suggested that the random distribution of tRNA genes is caused by reciprocal translocation between repetitive sequences mediated by a recBCD-like system.

Department of Biology Faculty of Medicine Masaryk University Kamenice 5 Building A6 62500 Brno Czech Republic

The Genome Institute Washington University in St Louis 4444 Forest Park Avenue St Louis MO 63108 USA

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Acinas S. G., Marcelino L. A., Klepac-Ceraj V., Polz M. F. PubMed DOI PMC

Antón A. I., Martínez-Murcia A. J., Rodríguez-Valera F. PubMed DOI

Baseman J. B., Nichols J. C., Rumpp J. W., Hayes N. S. PubMed PMC

Bunikis J., Garpmo U., Tsao J., Berglund J., Fish D., Barbour A. G. PubMed DOI

Čejková D., Zobaníková M., Chen L., Pospíšilová P., Strouhal M., Qin X., Mikalová L., Norris S. J., Muzny D. M. PubMed DOI PMC

Centurion-Lara A., Castro C., van Voorhis W. C., Lukehart S. A. PubMed DOI

Comstedt P., Asokliene L., Eliasson I., Olsen B., Wallensten A., Bunikis J., Bergström S. PubMed DOI PMC

Condon C., Philips J., Fu Z. Y., Squires C., Squires C. L. PubMed PMC

Darling A. E., Miklós I., Ragan M. A. PubMed DOI PMC

de Vries M. C., Siezen R. J., Wijman J. G., Zhao Y., Kleerebezem M., de Vos W. M., Vaughan E. E. PubMed DOI

Flasarová M., Šmajs D., Matějková P., Woznicová V., Heroldová-Dvoráková M., Votava M. PubMed

Flasarová M., Pospíšilová P., Mikalová L., Vališová Z., Dastychová E., Strnadel R., Kuklová I., Woznicová V., Zákoucká H., Šmajs D. PubMed

Fraser C. M., Casjens S., Huang W. M., Sutton G. G., Clayton R., Lathigra R., White O., Ketchum K. A., Dodson R. PubMed DOI

Fraser C. M., Norris S. J., Weinstock G. M., White O., Sutton G. G., Dodson R., Gwinn M., Hickey E. K., Clayton R. PubMed DOI

Fribourg-Blanc A., Mollaret H. H. PubMed

Fukunaga M., Okuzako N., Mifuchi I., Arimitsu Y., Seki M. PubMed

Gastinel P., Vaisman A., Hamelin A., Dunoyer F. PubMed

Giacani L., Jeffrey B. M., Molini B. J., Le H. T., Lukehart S. A., Centurion-Lara A., Rockey D. D. PubMed DOI PMC

Giacani L., Chattopadhyay S., Centurion-Lara A., Jeffrey B. M., Le H. T., Molini B. J., Lukehart S. A., Sokurenko E. V., Rockey D. D. PubMed DOI PMC

Gürtler V. PubMed DOI

Gürtler V., Stanisich V. A. PubMed DOI

Hanincová K., Liveris D., Sandigursky S., Wormser G. P., Schwartz I. PubMed DOI PMC

Hardy J. B., Hardy P. H., Oppenheimer E. H., Ryan S. J., Jr, Sheff R. N. PubMed DOI

Harvey S., Hill C. W. PubMed PMC

Hashimoto J. G., Stevenson B. S., Schmidt T. M. PubMed DOI PMC

Indra A., Blaschitz M., Kernbichler S., Reischl U., Wewalka G., Allerberger F. PubMed DOI PMC

Kobayashi I. PubMed DOI

Lan R. T., Reeves P. R. PubMed DOI

Lebuhn M., Bathe S., Achouak W., Hartmann A., Heulin T., Schloter M. PubMed DOI

Liao D. PubMed

Liska S. L., Perine P. L., Hunter E. F., Crawford J. A., Feeley J. C. DOI

Liu H., Rodes B., Chen C.-Y., Steiner B. PubMed DOI PMC

Liveris D., Wormser G. P., Nowakowski J., Nadelman R., Bittker S., Cooper D., Varde S., Moy F. H., Forseter G. PubMed PMC

Martin D. P., Lemey P., Lott M., Moulton V., Posada D., Lefeuvre P. PubMed DOI PMC

Matějková P., Strouhal M., Šmajs D., Norris S. J., Palzkill T., Petrosino J. F., Sodergren E., Norton J. E., Singh J. PubMed DOI PMC

Matějková P., Flasarová M., Zákoucká H., Boˇrek M., Kremenová S., Arenberger P., Woznicová V., Weinstock G. M., Šmajs D. PubMed DOI

Nei M., Rooney A. P. PubMed DOI PMC

Nichols H. J., Hough W. H. DOI

Pei A., Nossa C. W., Chokshi P., Blaser M. J., Yang L., Rosmarin D. M., Pei Z. PubMed DOI PMC

Pei A. Y., Oberdorf W. E., Nossa C. W., Agarwal A., Chokshi P., Gerz E. A., Jin Z., Lee P., Yang L. PubMed DOI PMC

Petes T. D., Hill C. W. PubMed DOI

Petit M.-A. DOI

Pillay A., Liu H., Chen C.-Y., Holloway B., Sturm A. W., Steiner B., Morse S. A. PubMed DOI

Posada D., Crandall K. A. PubMed DOI PMC

Rozen S., Skaletsky H. PubMed

Sadeghifard N., Gürtler V., Beer M., Seviour R. J. PubMed DOI PMC

Saitou N., Nei M. PubMed

Santoyo G., Romero D. PubMed

Sawyer S. PubMed

Schwartz J. J., Gazumyan A., Schwartz I. PubMed PMC

Seshadri R., Myers G. S., Tettelin H., Eisen J. A., Heidelberg J. F., Dodson R. J., Davidsen T. M., DeBoy R. T., Fouts D. E. PubMed DOI PMC

Šmajs D., Mikalová L., Čejková D., Strouhal M., Zobaníková M., Pospisilova P., Norris S. J., Weinstock G. M. DOI

Šmajs D., Zobaníková M., Strouhal M., Čejková D., Dugan-Rocha S., Pospís˜ilová P., Norris S. J., Albert T., Qin X. PubMed DOI PMC

Smith J. M. PubMed DOI

Stamm L. V., Bergen H. L. PubMed DOI PMC

Stamm L. V., Kerner T. C., Jr, Bankaitis V. A., Bassford P. J., Jr PubMed PMC

Stamm L. V., Bergen H. L., Walker R. L. PubMed DOI PMC

Stewart F. J., Cavanaugh C. M. PubMed DOI

Strouhal M., Šmajs D., Matějková P., Sodergren E., Amin A. G., Howell J. K., Norris S. J., Weinstock G. M. PubMed DOI PMC

Takahashi N. K., Yamamoto K., Kitamura Y., Luo S.-Q., Yoshikura H., Kobayashi I. PubMed DOI PMC

Tamura K., Dudley J., Nei M., Kumar S. PubMed DOI

Turner T. B., Hollander D. H. PubMed

Wendel G. D., Jr, Sánchez P. J., Peters M. T., Harstad T. W., Potter L. L., Norgard M. V. PubMed

Wormser G. P., Brisson D., Liveris D., Hanincová K., Sandigursky S., Nowakowski J., Nadelman R. B., Ludin S., Schwartz I. PubMed DOI PMC

Woznicová V., Šmajs D., Wechsler D., Matějková P., Flasarová M. PubMed DOI PMC

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