• Je něco špatně v tomto záznamu ?

Tviblindi algorithm identifies branching developmental trajectories of human B-cell development and describes abnormalities in RAG-1 and WAS patients

M. Bakardjieva, O. Pelák, M. Wentink, H. Glier, D. Novák, J. Stančíková, D. Kužílková, E. Mejstříková, I. Janowska, M. Rizzi, M. van der Burg, J. Stuchlý, T. Kalina

. 2024 ; 54 (12) : e2451004. [pub] 20240905

Jazyk angličtina Země Německo

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc25003283

Grantová podpora
23-05561S The Czech Science Foundation
23-07-00170 Czech Health Research Council
LX22NPO5102 European Union - Next Generation EU (Czech Recovery Plan) - Project National Cancer Research Institute
1S40421N FWO (Fonds Wetenschappelijk Onderzoek

Detailed knowledge of human B-cell development is crucial for the proper interpretation of inborn errors of immunity and malignant diseases. It is of interest to understand the kinetics of protein expression changes during development, but also to properly interpret the major and possibly alternative developmental trajectories. We have investigated human samples from healthy individuals with the aim of describing all B-cell developmental trajectories. We validated a 30-parameter mass cytometry panel and demonstrated the utility of "vaevictis" visualization of B-cell developmental stages. We used the trajectory inference tool "tviblindi" to exhaustively describe all trajectories leading to all developmental ends discovered in the data. Focusing on Natural Effector B cells, we demonstrated the dynamics of expression of nuclear factors (PAX-5, TdT, Ki-67, Bcl-2), cytokine and chemokine receptors (CD127, CXCR4, CXCR5) in relation to the canonical B-cell developmental stage markers. We observed branching of the memory development, where follicular memory formation was marked by CD73 expression. Lastly, we performed an analysis of two example cases of abnormal B-cell development caused by mutations in RAG-1 and Wiskott-Aldrich syndrome gene in patients with primary immunodeficiency. In conclusion, we developed, validated, and presented a comprehensive set of tools for the investigation of B-cell development in the bone marrow compartment.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc25003283
003      
CZ-PrNML
005      
20250206104224.0
007      
ta
008      
250121s2024 gw f 000 0|eng||
009      
AR
024    7_
$a 10.1002/eji.202451004 $2 doi
035    __
$a (PubMed)39235410
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a gw
100    1_
$a Bakardjieva, Marina $u CLIP, Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University, Prague, Czech Republic $1 https://orcid.org/0000000259196306
245    10
$a Tviblindi algorithm identifies branching developmental trajectories of human B-cell development and describes abnormalities in RAG-1 and WAS patients / $c M. Bakardjieva, O. Pelák, M. Wentink, H. Glier, D. Novák, J. Stančíková, D. Kužílková, E. Mejstříková, I. Janowska, M. Rizzi, M. van der Burg, J. Stuchlý, T. Kalina
520    9_
$a Detailed knowledge of human B-cell development is crucial for the proper interpretation of inborn errors of immunity and malignant diseases. It is of interest to understand the kinetics of protein expression changes during development, but also to properly interpret the major and possibly alternative developmental trajectories. We have investigated human samples from healthy individuals with the aim of describing all B-cell developmental trajectories. We validated a 30-parameter mass cytometry panel and demonstrated the utility of "vaevictis" visualization of B-cell developmental stages. We used the trajectory inference tool "tviblindi" to exhaustively describe all trajectories leading to all developmental ends discovered in the data. Focusing on Natural Effector B cells, we demonstrated the dynamics of expression of nuclear factors (PAX-5, TdT, Ki-67, Bcl-2), cytokine and chemokine receptors (CD127, CXCR4, CXCR5) in relation to the canonical B-cell developmental stage markers. We observed branching of the memory development, where follicular memory formation was marked by CD73 expression. Lastly, we performed an analysis of two example cases of abnormal B-cell development caused by mutations in RAG-1 and Wiskott-Aldrich syndrome gene in patients with primary immunodeficiency. In conclusion, we developed, validated, and presented a comprehensive set of tools for the investigation of B-cell development in the bone marrow compartment.
650    _2
$a lidé $7 D006801
650    12
$a B-lymfocyty $x imunologie $7 D001402
650    12
$a homeodoménové proteiny $x genetika $x metabolismus $7 D018398
650    12
$a buněčná diferenciace $x imunologie $x genetika $7 D002454
650    12
$a algoritmy $7 D000465
650    _2
$a mutace $7 D009154
655    _2
$a časopisecké články $7 D016428
700    1_
$a Pelák, Ondřej $u CLIP, Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Wentink, Marjolein $u Department of Internal Medicine, Erasmus MC, University Medical Center Rotterdam, Rotterdam, the Netherlands
700    1_
$a Glier, Hana $u CLIP, Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Novák, David $u CLIP, Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University, Prague, Czech Republic $u Department of Applied Mathematics, Computer Science and Statistics, Ghent University, Ghent, Belgium $u Data Mining and Modeling for Biomedicine, Center for Inflammation Research, VIB-UGent, Ghent, Belgium
700    1_
$a Stančíková, Jitka $u CLIP, Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Kužílková, Daniela $u CLIP, Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University, Prague, Czech Republic $u Department of Paediatric Haematology and Oncology, University Hospital Motol, Prague, Czech Republic $1 https://orcid.org/0000000180869790
700    1_
$a Mejstříková, Ester $u CLIP, Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University, Prague, Czech Republic $u Department of Paediatric Haematology and Oncology, University Hospital Motol, Prague, Czech Republic
700    1_
$a Janowska, Iga $u Department of Rheumatology and Clinical Immunology, Freiburg University Medical Center, University of Freiburg, Freiburg, Germany $u Center for Chronic Immunodeficiency, University Medical Center Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany $1 https://orcid.org/0009000475262803
700    1_
$a Rizzi, Marta $u Department of Rheumatology and Clinical Immunology, Freiburg University Medical Center, University of Freiburg, Freiburg, Germany $u Center for Chronic Immunodeficiency, University Medical Center Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany $1 https://orcid.org/0000000251536089
700    1_
$a van der Burg, Mirjam $u Department of Pediatrics, Laboratory for Pediatric Immmunology, Leiden University Medical Center, Leiden, the Netherlands $1 https://orcid.org/0000000215103104
700    1_
$a Stuchlý, Jan $u CLIP, Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University, Prague, Czech Republic $u Department of Paediatric Haematology and Oncology, University Hospital Motol, Prague, Czech Republic $1 https://orcid.org/0000000318969533 $7 xx0143777
700    1_
$a Kalina, Tomáš $u CLIP, Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University, Prague, Czech Republic $u Department of Paediatric Haematology and Oncology, University Hospital Motol, Prague, Czech Republic $1 https://orcid.org/0000000344752872 $7 xx0060125
773    0_
$w MED00001625 $t European journal of immunology $x 1521-4141 $g Roč. 54, č. 12 (2024), s. e2451004
856    41
$u https://pubmed.ncbi.nlm.nih.gov/39235410 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20250121 $b ABA008
991    __
$a 20250206104220 $b ABA008
999    __
$a ok $b bmc $g 2263186 $s 1239290
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2024 $b 54 $c 12 $d e2451004 $e 20240905 $i 1521-4141 $m European journal of immunology $n Eur J Immunol $x MED00001625
GRA    __
$a 23-05561S $p The Czech Science Foundation
GRA    __
$a 23-07-00170 $p Czech Health Research Council
GRA    __
$a LX22NPO5102 $p European Union - Next Generation EU (Czech Recovery Plan) - Project National Cancer Research Institute
GRA    __
$a 1S40421N $p FWO (Fonds Wetenschappelijk Onderzoek
LZP    __
$a Pubmed-20250121

Najít záznam

Citační ukazatele

Možnosti archivace