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RNA G-quadruplex formation in biologically important transcribed regions: can two-tetrad intramolecular RNA quadruplexes be formed

P. Basu, I. Kejnovská, M. Gajarský, D. Šubert, T. Mikešová, D. Renčiuk, L. Trantírek, JL. Mergny, M. Vorlíčková

. 2024 ; 52 (21) : 13224-13242. [pub] 20241127

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc25003377

Grantová podpora
GF22-04242L Czech Science and German Research Foundation
LM2023042 MEYS CR
CZ.02.1.01/0.0/0.0/18_046/0015974 European Regional Development Fund
nr. 22-29738S Czech Science Foundation
Inserm, CNRS, Ecole Polytechnique

G-quadruplexes (G4s) formed within RNA are emerging as promising targets for therapeutic intervention in cancer, neurodegenerative disorders and infectious diseases. Sequences containing a succession of short GG blocks, or uneven G-tract lengths unable to form three-tetrad G4s (GG motifs), are overwhelmingly more frequent than canonical motifs involving multiple GGG blocks. We recently showed that DNA is not able to form stable two-tetrad intramolecular parallel G4s. Whether RNA GG motifs can form intramolecular G4s under physiological conditions and play regulatory roles remains a burning question. In this study, we performed a systematic analysis and experimental evaluation of a number of biologically important RNA regions involving RNA GG motifs. We show that most of these motifs do not form stable intramolecular G4s but need to dimerize to form stable G4 structures. The strong tendency of RNA GG motif G4s to associate may participate in RNA-based aggregation under conditions of cellular stress.

Citace poskytuje Crossref.org

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