• Something wrong with this record ?

Elevated PD-L1 and PECAM-1 as Diagnostic Biomarkers of Acute Rejection in Lung Transplantation

R. Novysedlak, J. Balko, J. Tavandzis, V. Tovazhnianska, A. Slavcev, K. Vychytilova, J. Smetanova, A. Bohyn, J. Vajter, M. Borcinova, BM. Vanaudenaerde, R. Lischke, J. Vachtenheim, LJ. Ceulemans, Z. Ozaniak Strizova

. 2024 ; 37 (-) : 13796. [pub] 20241121

Language English Country Switzerland

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc25003412

Acute cellular rejection (ACR) frequently occurs following lung transplantation (LuTx) and represents a risk factor for the development of chronic lung allograft dysfunction (CLAD) as well as long-term survival. The histopathological diagnosis of ACR carries a burden of interobserver variability. The widespread utilization and cost-effectiveness of immunohistochemistry (IHC) was proven beneficial in diagnosing rejection in human kidney transplantations and LuTx rat models. However, its potential for ACR detection in patients remains unexplored. We analyzed surface markers (CD3, CD4, CD8, CD20, CD68, CD47, PD-1, PD-L1, and CD31/PECAM-1) on lung tissue cryobiopsy samples collected within 6 months post-LuTx from 60 LuTx recipients, 48 of whom were diagnosed with ACR. Additionally, serum samples from 51 patients were analyzed using a multiplex bead-based Luminex assay. The cytokines and markers included PD-L1, IL2, TNFα, IFNγ, and Granzyme B. We observed a significant increase in PD-L1 tissue expression within the rejection group, suggesting a concerted effort to suppress immune responses, especially those mediated by T-cells. Furthermore, we noted significant differences in PECAM-1 levels between ACR/non-ACR. Additionally, peripheral blood C-reactive-protein levels tended to be higher in the ACR group, while Luminex serum analyses did not reveal any significant differences between groups. In conclusion, our findings suggest the potential value of PECAM-1 and PD-L1 markers in diagnosing ACR.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc25003412
003      
CZ-PrNML
005      
20250206104328.0
007      
ta
008      
250121e20241121sz f 000 0|eng||
009      
AR
024    7_
$a 10.3389/ti.2024.13796 $2 doi
035    __
$a (PubMed)39640249
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Novysedlak, Rene $u Prague Lung Transplant Program, 3rd Department of Surgery, First Faculty of Medicine, Charles University and Motol University Hospital, Prague, Czechia $u Laboratory of Respiratory Diseases and Thoracic Surgery (BREATHE), Department of Chronic Diseases and Metabolism, KU Leuven, Leuven, Belgium
245    10
$a Elevated PD-L1 and PECAM-1 as Diagnostic Biomarkers of Acute Rejection in Lung Transplantation / $c R. Novysedlak, J. Balko, J. Tavandzis, V. Tovazhnianska, A. Slavcev, K. Vychytilova, J. Smetanova, A. Bohyn, J. Vajter, M. Borcinova, BM. Vanaudenaerde, R. Lischke, J. Vachtenheim, LJ. Ceulemans, Z. Ozaniak Strizova
520    9_
$a Acute cellular rejection (ACR) frequently occurs following lung transplantation (LuTx) and represents a risk factor for the development of chronic lung allograft dysfunction (CLAD) as well as long-term survival. The histopathological diagnosis of ACR carries a burden of interobserver variability. The widespread utilization and cost-effectiveness of immunohistochemistry (IHC) was proven beneficial in diagnosing rejection in human kidney transplantations and LuTx rat models. However, its potential for ACR detection in patients remains unexplored. We analyzed surface markers (CD3, CD4, CD8, CD20, CD68, CD47, PD-1, PD-L1, and CD31/PECAM-1) on lung tissue cryobiopsy samples collected within 6 months post-LuTx from 60 LuTx recipients, 48 of whom were diagnosed with ACR. Additionally, serum samples from 51 patients were analyzed using a multiplex bead-based Luminex assay. The cytokines and markers included PD-L1, IL2, TNFα, IFNγ, and Granzyme B. We observed a significant increase in PD-L1 tissue expression within the rejection group, suggesting a concerted effort to suppress immune responses, especially those mediated by T-cells. Furthermore, we noted significant differences in PECAM-1 levels between ACR/non-ACR. Additionally, peripheral blood C-reactive-protein levels tended to be higher in the ACR group, while Luminex serum analyses did not reveal any significant differences between groups. In conclusion, our findings suggest the potential value of PECAM-1 and PD-L1 markers in diagnosing ACR.
650    _2
$a lidé $7 D006801
650    12
$a transplantace plic $x škodlivé účinky $7 D016040
650    12
$a antigeny CD274 $x metabolismus $x krev $7 D060890
650    12
$a rejekce štěpu $x diagnóza $x krev $7 D006084
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a lidé středního věku $7 D008875
650    12
$a biologické markery $x krev $x metabolismus $7 D015415
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a dospělí $7 D000328
650    12
$a antigeny CD31 $x metabolismus $7 D019408
650    _2
$a akutní nemoc $7 D000208
650    _2
$a senioři $7 D000368
650    _2
$a plíce $x patologie $7 D008168
650    _2
$a imunohistochemie $7 D007150
655    _2
$a časopisecké články $7 D016428
700    1_
$a Balko, Jan $u Department of Pathology and Molecular Medicine, Second Faculty of Medicine, Charles University and Motol University Hospital, Prague, Czechia
700    1_
$a Tavandzis, Janis $u Prague Lung Transplant Program, 3rd Department of Surgery, First Faculty of Medicine, Charles University and Motol University Hospital, Prague, Czechia
700    1_
$a Tovazhnianska, Vira $u Department of Pathology and Molecular Medicine, Second Faculty of Medicine, Charles University and Motol University Hospital, Prague, Czechia
700    1_
$a Slavcev, Antonij $u Department of Immunogenetics, Institute for Clinical and Experimental Medicine, Prague, Czechia
700    1_
$a Vychytilova, Katerina $u Department of Immunogenetics, Institute for Clinical and Experimental Medicine, Prague, Czechia
700    1_
$a Smetanova, Jitka $u Department of Immunology, Second Faculty of Medicine, Charles University and Motol University Hospital, Prague, Czechia
700    1_
$a Bohyn, Alexandre $u Department of Public Health and Primary Care, Leuven Biostatistics and Statistical Bioinformatics Center (L-BioStat), KU Leuven, Leuven, Belgium
700    1_
$a Vajter, Jaromir $u Department of Anesthesiology, Resuscitation and Intensive Care Medicine, Second Faculty of Medicine, Charles University and Motol University Hospital, Prague, Czechia
700    1_
$a Borcinova, Martina $u Department of Obstetrics and Gynecology, First Faculty of Medicine, Charles University, General University Hospital, Prague, Czechia
700    1_
$a Vanaudenaerde, Bart M $u Laboratory of Respiratory Diseases and Thoracic Surgery (BREATHE), Department of Chronic Diseases and Metabolism, KU Leuven, Leuven, Belgium
700    1_
$a Lischke, Robert $u Prague Lung Transplant Program, 3rd Department of Surgery, First Faculty of Medicine, Charles University and Motol University Hospital, Prague, Czechia
700    1_
$a Vachtenheim, Jiri $u Prague Lung Transplant Program, 3rd Department of Surgery, First Faculty of Medicine, Charles University and Motol University Hospital, Prague, Czechia
700    1_
$a Ceulemans, Laurens J $u Laboratory of Respiratory Diseases and Thoracic Surgery (BREATHE), Department of Chronic Diseases and Metabolism, KU Leuven, Leuven, Belgium $u Department of Thoracic Surgery, University Hospitals Leuven, Leuven, Belgium
700    1_
$a Ozaniak Strizova, Zuzana $u Department of Immunology, Second Faculty of Medicine, Charles University and Motol University Hospital, Prague, Czechia
773    0_
$w MED00004553 $t Transplant international $x 1432-2277 $g Roč. 37 (20241121), s. 13796
856    41
$u https://pubmed.ncbi.nlm.nih.gov/39640249 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20250121 $b ABA008
991    __
$a 20250206104324 $b ABA008
999    __
$a ok $b bmc $g 2263284 $s 1239419
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2024 $b 37 $c - $d 13796 $e 20241121 $i 1432-2277 $m Transplant international $n Transpl Int $x MED00004553
LZP    __
$a Pubmed-20250121

Find record

Citation metrics

Archiving options