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Bacterial extracellular vesicles as intranasal postbiotics: Detailed characterization and interaction with airway cells
A. Razim, A. Zabłocka, A. Schmid, M. Thaler, V. Černý, T. Weinmayer, B. Whitehead, A. Martens, M. Skalska, M. Morandi, K. Schmidt, ME. Wysmołek, A. Végvári, D. Srutkova, M. Schwarzer, L. Neuninger, P. Nejsum, J. Hrdý, J. Palmfeldt, M. Brucale, F....
Language English Country United States
Document type Journal Article
Grant support
PPN/BAT/2021/1/00004/U/00001
Narodowa Agencja Wymiany Akademickiej
CZ.02.01.01/00/22_010/0008115
OP JAK project MSCA fellowships CZ-UK2
23-04050L
Grantová Agentura České Republiky
Danube-Allergy Cluster (P17)
Amt der NÖ Landesregierung
CZ 04/2024
OeAD-GmbH
CZ 07/2023
OeAD-GmbH
CZ 15/2023
OeAD-GmbH
RS 08/2022
OeAD-GmbH
10.47379/LS20025
Vienna Science and Technology Fund
LM/29/SM
SciMat and qLife Priority Research Area under Strategic Programme Excellence Initiative "Laboratories for the Young"
P 34867
Austrian Science Fund
101066450
HORIZON EUROPE Marie Sklodowska-Curie Actions
CZ.02.01.01/00/22_008/0004597
Ministry of Education, Youth and Sports of the Czech Republic
NLK
Directory of Open Access Journals
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PubMed Central
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from 2012-01-01
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PubMed
39429019
DOI
10.1002/jev2.70004
Knihovny.cz E-resources
- MeSH
- Administration, Intranasal * MeSH
- Epithelial Cells metabolism MeSH
- Escherichia coli * metabolism MeSH
- Extracellular Vesicles * metabolism MeSH
- Humans MeSH
- Lymphoid Tissue metabolism MeSH
- Macrophages metabolism MeSH
- Mice MeSH
- NF-kappa B metabolism MeSH
- Oxidative Stress MeSH
- Lung microbiology metabolism MeSH
- Probiotics * administration & dosage MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Escherichia coli A0 34/86 (EcO83) is a probiotic strain used in newborns to prevent nosocomial infections and diarrhoea. This bacterium stimulates both pro- and anti-inflammatory cytokine production and its intranasal administration reduces allergic airway inflammation in mice. Despite its benefits, there are concerns about the use of live probiotic bacteria due to potential systemic infections and gene transfer. Extracellular vesicles (EVs) derived from EcO83 (EcO83-EVs) might offer a safer alternative to live bacteria. This study characterizes EcO83-EVs and investigates their interaction with host cells, highlighting their potential as postbiotic therapeutics. EcO83-EVs were isolated, purified, and characterised following the Minimal Information of Studies of Extracellular Vesicles (MISEV) guidelines. Ex vivo studies conducted in human nasal epithelial cells showed that EcO83-EVs increased the expression of proteins linked to oxidative stress and inflammation, indicating an effective interaction between EVs and the host cells. Further in vivo studies in mice demonstrated that EcO83-EVs interact with nasal-associated lymphoid tissue, are internalised by airway macrophages, and stimulate neutrophil recruitment in the lung. Mechanistically, EcO83-EVs activate the NF-κΒ signalling pathway, resulting in the nitric oxide production. EcO83-EVs demonstrate significant potential as a postbiotic alternative to live bacteria, offering a safer option for therapeutic applications. Further research is required to explore their clinical use, particularly in mucosal vaccination and targeted immunotherapy strategies.
Department of Clinical Medicine Aarhus University Aarhus Denmark
Department of Infectious Diseases Aarhus University Hospital Aarhus Denmark
Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences Wroclaw Poland
Institute of Nanostructured Materials CNR ISMN Bologna Italy
References provided by Crossref.org
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