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Flexible 3D nanofiber-based SERS biosensor for detection of miRNA-223-3p in early Laryngeal Cancer diagnosis

S. Martino, D. Yilmaz, C. Tammaro, G. Misso, A. Esposito, M. Falco, AM. Cossu, A. Lombardi, E. Amler, R. Divin, A. Giannetti, M. Scrima, P. Dardano, L. De Stefano, I. Rea, AC. De Luca, M. Caraglia

. 2025 ; 285 (-) : 127293. [pub] 20241129

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc25009388

MicroRNAs (miRNAs) are small non-coding RNAs (18-22 nucleotides) that regulate gene expression and are associated with various diseases, including Laryngeal Cancer (LCa), which has a high mortality rate due to late diagnosis. Traditional methods for miRNA detection present several drawbacks (time-consuming steps, high cost and high false positive rate). Early-stage diagnosis and selective detection of miRNAs remain challenging. This study proposes a 3D flexible biosensor that combines nanofibers (NFs), gold nanoparticles (AuNPs), and an inverse molecular sentinel (iMS) for enzyme-free, SERS-based detection of miRNA-223-3p, evaluated as a potential LCa biomarker. The electrospun flexible nanofibers decorated with AuNPs enhance Raman signal. Selective detection of miRNA-223-3p is achieved by immobilizing an iMS-DNA probe labeled with a Raman reporter (Cyanine 3) on the AuNPs. The iMS distinctive stem-and-loop structure undergoes a conformational change upon interaction with the miRNA-223-3p, producing an "on to off" SERS signal. The proposed sensor demonstrated a linear detection range from 10 to 250 fM, with a limit of detection (LOD) of 19.50 ± 0.05 fM. The sensor selectivity was confirmed by analyzing the SERS signal behaviour in the presence of both Non-complementary miRNA and miRNA with three mismatched base pairs. This easily fabricable sensor requires no amplification and offers key advantages, including sensitivity, flexibility, and cost-effectiveness.

Citace poskytuje Crossref.org

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$a MicroRNAs (miRNAs) are small non-coding RNAs (18-22 nucleotides) that regulate gene expression and are associated with various diseases, including Laryngeal Cancer (LCa), which has a high mortality rate due to late diagnosis. Traditional methods for miRNA detection present several drawbacks (time-consuming steps, high cost and high false positive rate). Early-stage diagnosis and selective detection of miRNAs remain challenging. This study proposes a 3D flexible biosensor that combines nanofibers (NFs), gold nanoparticles (AuNPs), and an inverse molecular sentinel (iMS) for enzyme-free, SERS-based detection of miRNA-223-3p, evaluated as a potential LCa biomarker. The electrospun flexible nanofibers decorated with AuNPs enhance Raman signal. Selective detection of miRNA-223-3p is achieved by immobilizing an iMS-DNA probe labeled with a Raman reporter (Cyanine 3) on the AuNPs. The iMS distinctive stem-and-loop structure undergoes a conformational change upon interaction with the miRNA-223-3p, producing an "on to off" SERS signal. The proposed sensor demonstrated a linear detection range from 10 to 250 fM, with a limit of detection (LOD) of 19.50 ± 0.05 fM. The sensor selectivity was confirmed by analyzing the SERS signal behaviour in the presence of both Non-complementary miRNA and miRNA with three mismatched base pairs. This easily fabricable sensor requires no amplification and offers key advantages, including sensitivity, flexibility, and cost-effectiveness.
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$a Cossu, Alessia Maria $u Department of Precision Medicine, University of Campania "Luigi Vanvitelli", 80138, Naples, Italy; Laboratory of Molecular and Precision Oncology, Biogem Scarl, Institute of Genetic Research, 83031, Ariano Irpino, Italy
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$a Lombardi, Angela $u U.P. Cytometric and Mutational Diagnostics, AOU Policlinico, University of Campania "Luigi Vanvitelli", Via Luciano Armanni 5, 83031, Naples, Italy
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$a Divin, Radek $u Institute of Biophysics, Second Faculty of Medicine, Charles University in Prague, V Úvalu 84, 150 06, Prague, Czech Republic; University Centre for Energy Efficient Buildings, Czech Technical University in Prague, Trinecka 1024, 27343, Bustehrad, Czech Republic
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$a Giannetti, Ambra $u Institute of Applied Physics "Nello Carrara", National Research Council, 50019, Sesto Fiorentino, FI, Italy
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