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Guidelines for minimal reporting requirements, design and interpretation of experiments involving the use of eukaryotic dual gene expression reporters (MINDR)
G. Loughran, DE. Andreev, IM. Terenin, O. Namy, M. Mikl, MM. Yordanova, CJ. McManus, AE. Firth, JF. Atkins, CS. Fraser, Z. Ignatova, S. Iwasaki, J. Kufel, O. Larsson, SA. Leidel, AS. Mankin, M. Mariotti, ME. Tanenbaum, I. Topisirovic, N....
Language English Country United States
Document type Journal Article, Review
- MeSH
- Eukaryota genetics MeSH
- Humans MeSH
- RNA, Messenger genetics metabolism MeSH
- Protein Biosynthesis genetics MeSH
- Genes, Reporter * MeSH
- Guidelines as Topic MeSH
- Research Design standards MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Review MeSH
Dual reporters encoding two distinct proteins within the same mRNA have had a crucial role in identifying and characterizing unconventional mechanisms of eukaryotic translation. These mechanisms include initiation via internal ribosomal entry sites (IRESs), ribosomal frameshifting, stop codon readthrough and reinitiation. This design enables the expression of one reporter to be influenced by the specific mechanism under investigation, while the other reporter serves as an internal control. However, challenges arise when intervening test sequences are placed between these two reporters. Such sequences can inadvertently impact the expression or function of either reporter, independent of translation-related changes, potentially biasing the results. These effects may occur due to cryptic regulatory elements inducing or affecting transcription initiation, splicing, polyadenylation and antisense transcription as well as unpredictable effects of the translated test sequences on the stability and activity of the reporters. Unfortunately, these unintended effects may lead to misinterpretation of data and the publication of incorrect conclusions in the scientific literature. To address this issue and to assist the scientific community in accurately interpreting dual-reporter experiments, we have developed comprehensive guidelines. These guidelines cover experimental design, interpretation and the minimal requirements for reporting results. They are designed to aid researchers conducting these experiments as well as reviewers, editors and other investigators who seek to evaluate published data.
Belozersky Institute of Physico Chemical Biology Lomonosov Moscow State University Moscow Russia
Center for Biomolecular Sciences University of Illinois Chicago Chicago IL USA
Department of Biochemistry and Molecular Biophysics Columbia University New York NY USA
Department of Biochemistry McGill University Montréal Quebec Canada
Department of Biological Sciences Carnegie Mellon University Pittsburgh PA USA
Department of Biological Sciences University of Maryland Baltimore County Baltimore MD USA
Department of Biology Texas A and M University College Station TX USA
Department of Bionanoscience Delft University of Technology Delft the Netherlands
Department of Cell Biology and Molecular Genetics University of Maryland College Park MD USA
Department of Chemistry and Biochemistry University of Notre Dame Notre Dame IN USA
Department of Chemistry Biochemistry and Pharmaceutical Sciences University of Bern Bern Switzerland
Department of Human Biology University of Haifa Haifa Israel
Department of Medicine Harvard Medical School Boston MA USA
Department of Molecular Biophysics and Biochemistry Yale School of Medicine New Haven CT USA
Department of Oncology Pathology Science for Life Laboratory Karolinska Institute Stockholm Sweden
Division of Experimental Medicine McGill University Montréal Quebec Canada
Division of Rheumatology Inflammation and Immunity Brigham and Women's Hospital Boston MA USA
EIRNA Bio Bioinnovation Hub Cork Ireland
Faculty of Biology and Preclinical Medicine University of Regensburg Regensburg Germany
Gerald Bronfman Department of Oncology McGill University Montréal Quebec Canada
Institute for Bioscience and Biotechnology Research Rockville MD USA
Institute for Integrative Biology of the Cell CEA Université Paris Saclay CNRS Gif sur Yvette France
Institute of Biochemistry and Molecular Biology University of Hamburg Hamburg Germany
Institute of Biophysics National Research Council Unit Povo Italy
Institute of Genetics and Biotechnology Faculty of Biology University of Warsaw Warsaw Poland
Lady Davis Institute McGill University Montréal Quebec Canada
MRC Toxicology Unit University of Cambridge Cambridge UK
New York Structural Biology Center New York NY USA
RNA Bioscience Initiative University of Colorado Anschutz Medical Campus Aurora CO USA
RNA Systems Biochemistry Laboratory RIKEN Cluster for Pioneering Research Wako Japan
School of Biochemistry and Cell Biology University College Cork Cork Ireland
Shemyakin Ovchinnikov Institute of Bioorganic Chemistry RAS Moscow Russia
References provided by Crossref.org
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