-
Something wrong with this record ?
The kinetics of uracil-N-glycosylase distribution inside replication foci
A. Ligasová, I. Frydrych, B. Piskláková, D. Friedecký, K. Koberna
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
NU22-08-00148
Ministerstvo Zdravotnictví Ceské Republiky
TN01000013
Technologická Agentura České Republiky
EXCELES, grant number LX22NPO5102
Ministerstvo Školství, Mládeže a Tělovýchovy
NLK
Directory of Open Access Journals
from 2011
Free Medical Journals
from 2011
Nature Open Access
from 2011-12-01
PubMed Central
from 2011
Europe PubMed Central
from 2011
ProQuest Central
from 2011-01-01
Open Access Digital Library
from 2011-01-01
Open Access Digital Library
from 2011-01-01
Health & Medicine (ProQuest)
from 2011-01-01
ROAD: Directory of Open Access Scholarly Resources
from 2011
Springer Nature OA/Free Journals
from 2011-12-01
- MeSH
- Cell Nucleus metabolism MeSH
- Cell Cycle MeSH
- Kinetics MeSH
- Humans MeSH
- DNA Repair MeSH
- DNA Replication * MeSH
- S Phase MeSH
- Uracil-DNA Glycosidase * metabolism MeSH
- Uracil metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Mismatched nucleobase uracil is commonly repaired through the base excision repair initiated by DNA uracil glycosylases. The data presented in this study strongly indicate that the nuclear uracil-N-glycosylase activity and nuclear protein content in human cell lines is highest in the S phase of the cell cycle and that its distribution kinetics partially reflect the DNA replication activity in replication foci. In this respect, the data demonstrate structural changes of the replication focus related to the uracil-N-glycosylase distribution several dozens of minutes before end of its replication. The analysis also showed that very popular synchronisation protocols based on the double thymidine block can result in changes in the UNG2 content and uracil excision rate. In response, we propose a new method for the description of the changes of the content and the activity of different cell components during cell cycle without the necessity to use synchronisation protocols.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc25010197
- 003
- CZ-PrNML
- 005
- 20250429135320.0
- 007
- ta
- 008
- 250415s2025 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1038/s41598-024-84408-x $2 doi
- 035 __
- $a (PubMed)39849039
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Ligasová, Anna $u Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University Olomouc, Olomouc, Czech Republic. anna.ligasova@upol.cz
- 245 14
- $a The kinetics of uracil-N-glycosylase distribution inside replication foci / $c A. Ligasová, I. Frydrych, B. Piskláková, D. Friedecký, K. Koberna
- 520 9_
- $a Mismatched nucleobase uracil is commonly repaired through the base excision repair initiated by DNA uracil glycosylases. The data presented in this study strongly indicate that the nuclear uracil-N-glycosylase activity and nuclear protein content in human cell lines is highest in the S phase of the cell cycle and that its distribution kinetics partially reflect the DNA replication activity in replication foci. In this respect, the data demonstrate structural changes of the replication focus related to the uracil-N-glycosylase distribution several dozens of minutes before end of its replication. The analysis also showed that very popular synchronisation protocols based on the double thymidine block can result in changes in the UNG2 content and uracil excision rate. In response, we propose a new method for the description of the changes of the content and the activity of different cell components during cell cycle without the necessity to use synchronisation protocols.
- 650 _2
- $a lidé $7 D006801
- 650 12
- $a replikace DNA $7 D004261
- 650 _2
- $a kinetika $7 D007700
- 650 12
- $a uracil-DNA-glykosidasa $x metabolismus $7 D051981
- 650 _2
- $a buněčný cyklus $7 D002453
- 650 _2
- $a oprava DNA $7 D004260
- 650 _2
- $a uracil $x metabolismus $7 D014498
- 650 _2
- $a S fáze $7 D016196
- 650 _2
- $a buněčné jádro $x metabolismus $7 D002467
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Frydrych, Ivo $u Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University Olomouc, Olomouc, Czech Republic
- 700 1_
- $a Piskláková, Barbora $u Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University Olomouc, Olomouc, Czech Republic $u Laboratory of Inherited Metabolic Disorders, Department of Clinical Chemistry, Palacký University and University Hospital Olomouc, Olomouc, Czech Republic
- 700 1_
- $a Friedecký, David $u Laboratory of Inherited Metabolic Disorders, Department of Clinical Chemistry, Palacký University and University Hospital Olomouc, Olomouc, Czech Republic
- 700 1_
- $a Koberna, Karel $u Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University Olomouc, Olomouc, Czech Republic. karel.koberna@upol.cz
- 773 0_
- $w MED00182195 $t Scientific reports $x 2045-2322 $g Roč. 15, č. 1 (2025), s. 3026
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/39849039 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y - $z 0
- 990 __
- $a 20250415 $b ABA008
- 991 __
- $a 20250429135316 $b ABA008
- 999 __
- $a ok $b bmc $g 2311519 $s 1247278
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2025 $b 15 $c 1 $d 3026 $e 20250124 $i 2045-2322 $m Scientific reports $n Sci Rep $x MED00182195
- GRA __
- $a NU22-08-00148 $p Ministerstvo Zdravotnictví Ceské Republiky
- GRA __
- $a TN01000013 $p Technologická Agentura České Republiky
- GRA __
- $a EXCELES, grant number LX22NPO5102 $p Ministerstvo Školství, Mládeže a Tělovýchovy
- LZP __
- $a Pubmed-20250415
