Phosphate uptake and involvement of binding protein in Tween-80 supplemented culture of Aspergillus fumigatus
Language English Country United States Media print
Document type Journal Article
PubMed
18385
DOI
10.1007/bf02885603
Knihovny.cz E-resources
- MeSH
- Aspergillus fumigatus drug effects metabolism MeSH
- Dinitrophenols pharmacology MeSH
- Nitrates pharmacology MeSH
- Phosphates metabolism MeSH
- Fungal Proteins metabolism MeSH
- Hydrogen-Ion Concentration MeSH
- Cyanides pharmacology MeSH
- Osmosis MeSH
- Polyethylene Glycols pharmacology MeSH
- Polysorbates pharmacology MeSH
- Pronase pharmacology MeSH
- Carrier Proteins metabolism MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Dinitrophenols MeSH
- Nitrates MeSH
- Phosphates MeSH
- Fungal Proteins MeSH
- Cyanides MeSH
- Polyethylene Glycols MeSH
- Polysorbates MeSH
- Pronase MeSH
- Carrier Proteins MeSH
Tween-80 supplementation in submerged culture of Aspergillus fumigatus resulted in an increase of phosphate uptake. The uptake system was characterized as saturable, energy-dependent and operating against the concentration gradient. Control and Tween 80 cultures showed similar Km values for phosphate uptake (50 micrometer). Cold osmotic shock treatment of the cultures was found to cause considerable reduction in the ability to take up phosphorus with concomitant release of the binding protein into the shock fluid. Binding protein preparation from Tween-80 supplemented cells showed more activity than that from control cells.
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